Oxalic acid-induced resistance to Rhizoctonia solani in rice is associated with induction of phenolics, peroxidase and pathogenesis-related proteins

Jayaraman Jayaraj, Ranganathan Bhuvaneswari, Ramalingam Rabindran, Subbaratnam Muthukrishnan, Rethinasamy Velazhahan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Oxalic acid (1 mM) when applied as a foliar spray to rice plants induced resistance to challenge infection with Rhizoctonia solani, the rice sheath blight pathogen. Maximum reduction in sheath blight incidence was observed when the plants were sprayed with oxalic acid three days before inoculation with the fungus. The biochemical alterations in rice plants treated with oxalic acid was also investigated. When rice plants were treated with oxalic acid, a two-fold increase in phenolic content in leaf sheaths was recorded three days after treatment. Phenylalanine ammonialyase and peroxidase activities increased significantly starting from two days after treatment. Peroxidase (PO) isozyme analysis indicated that PO-3 and PO-4 were induced two days after treatment with oxalic acid. Western blot analysis revealed that two chitinases (28 and 35 kDa) and two β-1,3-glucanases (30 and 32 kDa) were strongly induced in rice sheaths four to six days after treatment with oxalic acid. Immunoblot analysis of protein extracts from oxalic acid-treated plants demonstrated the induction of a 23 kDa thaumatin-like protein (TLP) cross-reacting with bean TLP antibody. These results suggest that the enhanced activities of defense enzymes and defense-related compounds in oxalic acid-treated rice plants may contribute to resistance against R. solani.

Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalJournal of Plant Interactions
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 2010

Keywords

  • Defence enzymes
  • Induced resistance
  • Oryza sativa
  • Oxalic acid
  • Pathogenesis-related proteins
  • Sheath blight

ASJC Scopus subject areas

  • Plant Science
  • Ecology, Evolution, Behavior and Systematics

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