TY - JOUR
T1 - Nabag (Zizyphus spina-christi) extract prevents aberrant crypt foci development in colons of azoxymethane-treated rats by abrogating oxidative stress and inducing apoptosis
AU - Guizani, Nejib
AU - Waly, Mostafa Ibrahim
AU - Singh, Vandita
AU - Rahman, Mohammad Shafiur
PY - 2013
Y1 - 2013
N2 - Zizyphus spina-christi (ZSC) fruit is a rich source of bioactive compounds but any medicinal properties in chemoprevention of colon cancer have hitherto not been studied. The aim of the present study was to examine in vivo protective effects of ZSC water extract on colon carcinogenesis in azoxymethane (AOM)-treated rats. Our results showed that ZSC significantly reduced AOM-induced colonic aberrant crypt foci development and AOM-induced oxidative stress as indicated by restoration of endogenous glutathione depletion and abrogating the impairment of total antioxidant capacity. Caspase-3 cleavage, which has been considered as an apoptotic index, was almost undetectable in AOM-treated rats and ZSC exhibited pro-apoptotic effects evidenced by increased levels of cleaved caspase-3. In the studied model, our findings provide the first in vivo evidence that ZSC extract could inhibit the early stage of colon carcinogenesis by preventing oxidative stress and inducing apoptosis.
AB - Zizyphus spina-christi (ZSC) fruit is a rich source of bioactive compounds but any medicinal properties in chemoprevention of colon cancer have hitherto not been studied. The aim of the present study was to examine in vivo protective effects of ZSC water extract on colon carcinogenesis in azoxymethane (AOM)-treated rats. Our results showed that ZSC significantly reduced AOM-induced colonic aberrant crypt foci development and AOM-induced oxidative stress as indicated by restoration of endogenous glutathione depletion and abrogating the impairment of total antioxidant capacity. Caspase-3 cleavage, which has been considered as an apoptotic index, was almost undetectable in AOM-treated rats and ZSC exhibited pro-apoptotic effects evidenced by increased levels of cleaved caspase-3. In the studied model, our findings provide the first in vivo evidence that ZSC extract could inhibit the early stage of colon carcinogenesis by preventing oxidative stress and inducing apoptosis.
KW - Azoxymethane
KW - Colon cancer
KW - Colonic cells apoptosis
KW - Glutathione
KW - Zizyphus spina-christi
UR - http://www.scopus.com/inward/record.url?scp=84887574746&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887574746&partnerID=8YFLogxK
U2 - 10.7314/APJCP.2013.14.9.5031
DO - 10.7314/APJCP.2013.14.9.5031
M3 - Article
C2 - 24175771
AN - SCOPUS:84887574746
SN - 1513-7368
VL - 14
SP - 5031
EP - 5035
JO - Asian Pacific Journal of Cancer Prevention
JF - Asian Pacific Journal of Cancer Prevention
IS - 9
ER -