TY - JOUR
T1 - Morphological and chromosomal abnormalities in gamma radiation-induced mutagenized faba bean genotypes
AU - Nurmansyah, Mahdi
AU - Alghamdi, Salem S.
AU - Migdadi, Hussein M.
AU - Farooq, Muhammad
N1 - Funding Information:
The authors extend their appreciation to the International Scientific Partnership Program (ISPP) at King Saud University for funding this research work through ISPP# 0085.
Publisher Copyright:
© 2018 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Purpose: This study was conducted to evaluate and compare the influence of gamma radiations on morphological and chromosomal abnormalities in twenty mutagenized faba bean populations, representing first and second generations (M1 and M2) of five faba bean genotypes. Materials and methods: Five faba bean genotypes were exposed at two doses of gamma radiations (25 and 50 Gy). For determining the types of chromosomal aberrations caused by the gamma radiation, mitotic and meiotic cells were isolated from root tips and pollen mother cells, respectively. Results: The M1 generations of the five genotypes varied for sensitivity to gamma radiations, for seedling emergence. The genotype Skah 2 was more sensitive than other genotypes, the order of sensitivity of other genotypes was Misr 3 > ILB 4347 > Hassawi 2 > Hassawi 3. However, seedling emergence of the M2 generations was not as much reduced as that of the M1 generations. Ten different chlorophyll-deficient mutants were identified among the M2 generations. Gamma radiations also caused the development of abnormal leaflets, flowers and pollen grains. The most common types of chromosome aberrations in the mitotic cells were stickiness, laggard and chromosome breaks, whereas the most common types in the meiotic cells were stickiness and disturbed polarity. Conclusion: The gamma radiation decreased the seedling emergence and induced a wide range of morphological and chromosomal abnormalities in faba bean.
AB - Purpose: This study was conducted to evaluate and compare the influence of gamma radiations on morphological and chromosomal abnormalities in twenty mutagenized faba bean populations, representing first and second generations (M1 and M2) of five faba bean genotypes. Materials and methods: Five faba bean genotypes were exposed at two doses of gamma radiations (25 and 50 Gy). For determining the types of chromosomal aberrations caused by the gamma radiation, mitotic and meiotic cells were isolated from root tips and pollen mother cells, respectively. Results: The M1 generations of the five genotypes varied for sensitivity to gamma radiations, for seedling emergence. The genotype Skah 2 was more sensitive than other genotypes, the order of sensitivity of other genotypes was Misr 3 > ILB 4347 > Hassawi 2 > Hassawi 3. However, seedling emergence of the M2 generations was not as much reduced as that of the M1 generations. Ten different chlorophyll-deficient mutants were identified among the M2 generations. Gamma radiations also caused the development of abnormal leaflets, flowers and pollen grains. The most common types of chromosome aberrations in the mitotic cells were stickiness, laggard and chromosome breaks, whereas the most common types in the meiotic cells were stickiness and disturbed polarity. Conclusion: The gamma radiation decreased the seedling emergence and induced a wide range of morphological and chromosomal abnormalities in faba bean.
KW - chromosome aberration
KW - Faba bean
KW - gamma radiation
KW - morphological abnormality
KW - mutagenized population
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U2 - 10.1080/09553002.2018.1409913
DO - 10.1080/09553002.2018.1409913
M3 - Article
C2 - 29185843
AN - SCOPUS:85037740192
SN - 0955-3002
VL - 94
SP - 174
EP - 185
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 2
ER -