Monitoring of West Nile Virus Infections in Germany

U. Ziegler, D. Seidowski, J. Angenvoort, M. Eiden, K. Müller, N. Nowotny, M. H. Groschup

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

West Nile virus (WNV) is a flavivirus that is maintained in an enzootic cycle between ornithophilic mosquitoes, mainly of the Culex genus, and certain wild bird species. Other bird species like ravens, jays and raptors are highly susceptible to the infection and may develop deadly encephalitis, while further species of birds are only going through subclinical infection. The objective of this study was to continue in years 2009-2011 the serological and molecular surveillance in wild birds in Germany (see Vector Borne Zoonotic Dis. 10, 639) and to expand these investigations for the first time also to sera from domestic poultry and horses collected between 2005 and 2009. All three cohorts function as indicators for the endemic circulation of WNV. The presence of WNV-specific antibodies was detected in all samples by virus neutralization test (VNT), indirect immunofluorescence test (IFT) and/or enzyme-linked immunosorbent assay (ELISA). The presence of WNV genomes was monitored in relevant sera using two qRT-PCRs that amplify lineage 1 and 2 strains. A total of 364 migratory and resident wild bird serum samples (with emphasis on Passeriformes and Falconiformes) as well as 1119 serum samples from domestic poultry and 1282 sera from horses were analysed. With the exception of one hooded crow, antibody carriers were exclusively found in migratory birds, but not in resident birds/domestic poultry or in local horses. Crows are facultative, short-distance winter migrants in Germany. WNV-specific nucleic acids could not be demonstrated in any of the samples. According to these data, there is no convincing evidence for indigenous WNV infections in equines and in wild/domestic birds in Germany. However, since a few years, WNV infections are endemic in other European countries such as Austria, Hungary, Greece and Italy, a state-of-the-art surveillance system for the detection of incursions of WNV into Germany deems mandatory.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalZoonoses and Public Health
Volume59
Issue numberSUPPL.2
DOIs
Publication statusPublished - Sep 2012

Fingerprint

West Nile virus
Virus Diseases
Birds
Germany
monitoring
Crows
infection
wild birds
Poultry
Horses
Serum
poultry
crows
birds
horses
Falconiformes
Austria-Hungary
Passeriformes
Raptors
Flavivirus

Keywords

  • Arbovirus(es)
  • Birds
  • Germany
  • Horses
  • Monitoring
  • West Nile virus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Epidemiology
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Ziegler, U., Seidowski, D., Angenvoort, J., Eiden, M., Müller, K., Nowotny, N., & Groschup, M. H. (2012). Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health, 59(SUPPL.2), 95-101. https://doi.org/10.1111/zph.12015

Monitoring of West Nile Virus Infections in Germany. / Ziegler, U.; Seidowski, D.; Angenvoort, J.; Eiden, M.; Müller, K.; Nowotny, N.; Groschup, M. H.

In: Zoonoses and Public Health, Vol. 59, No. SUPPL.2, 09.2012, p. 95-101.

Research output: Contribution to journalArticle

Ziegler, U, Seidowski, D, Angenvoort, J, Eiden, M, Müller, K, Nowotny, N & Groschup, MH 2012, 'Monitoring of West Nile Virus Infections in Germany', Zoonoses and Public Health, vol. 59, no. SUPPL.2, pp. 95-101. https://doi.org/10.1111/zph.12015
Ziegler U, Seidowski D, Angenvoort J, Eiden M, Müller K, Nowotny N et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health. 2012 Sep;59(SUPPL.2):95-101. https://doi.org/10.1111/zph.12015
Ziegler, U. ; Seidowski, D. ; Angenvoort, J. ; Eiden, M. ; Müller, K. ; Nowotny, N. ; Groschup, M. H. / Monitoring of West Nile Virus Infections in Germany. In: Zoonoses and Public Health. 2012 ; Vol. 59, No. SUPPL.2. pp. 95-101.
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