Molecular surveillance of Theileria parasites of livestock in Oman

Amira Al-Fahdi, Badar Alqamashoui, Salama Al-Hamidhi, Onur Kose, Mohammed H. Tageldin, Patrick Bobade, Eugene H. Johnson, Abdel Rahim Hussain, Tulin Karagenc, Andy Tait, Brian Shiels, Huseyin Bilgin Bilgic, Hamza Babiker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Theileriosis is one of the most prevalent infectious diseases of livestock in the Arabian Peninsula, and causes high rates of mortality and morbidity in sheep and cattle. However, there is a paucity of information on the distribution of Theileria spp. over the whole region and their impact on different hosts. The present study carried out a country-wide molecular survey for Theileria spp. of livestock in Oman across four governorates. The aim of the survey was to define the prevalence of Theileria spp. in cattle, sheep and goats, highlight risk factors for infection and identify the main tick species involved in parasite transmission. Material and methods: A total of 2020 animals were examined in the survey consisting of sheep [n = 592], goats [n = 981] and cattle [n = 447]. All three species were raised and co-grazed on the same farms. Theileria parasites were detected using PCR-RFLP and RLB of the 18S rRNA gene. Cloning and sequencing of the 18S rRNA was carried out on 11 T. lestoquardi isolates from Ash-Sharqiyah, and Ad-Dhahira governorates, and phylogenetic relationships were inferred using additional sequences of T. lestoquardi, T. annulata and T. ovis available in GenBank. Results: Theileria spp. prevalence was 72.3%, 36.7% and 2.7% among cattle, sheep and goats, respectively. Strong similarity in results was obtained using RLB and PCR-RFLP for detection of Theileria spp. however, RLB detected a higher rate of mixed infection than PCR-RFPL (P. <. 0.001). Theileria annulata was the only parasite detected in cattle, while sheep and goats carried T. ovis, T. lestoquardi and T. annulata as well as Theileria spp. OT1. Of the four Theileria spp. detected in small ruminants, overall T. ovis was most prevalent (sheep [33.4%], goats [2.0%]), whereas T. lestoquardi was less prevalent (sheep [22.0%], goats [0.5%]). A large proportion of infected sheep (19%) carried mixed infection of T. ovis and T. lestoquardi. However, single T. lestoquardi infections (3.0%) were less prevalent than T. ovis infections (14.5%). Risk of Theileria spp. infection was significantly higher for exotic breeds, relative to native breeds, of cattle (p = 0.00002) and sheep (p = 0.005). Phylogenetic analysis placed T. lestoquardi in Oman in the same clade as other T. lestoquardi strains isolated from the same regional area (Iraq and Iran). The main tick species, identified on the examined animals. , Hyalomma anatolicum, was widely distributed and was found in all of the surveyed governorates. Conclusion: Theileria spp. are widespread in Oman with variable prevalence detected in different regions. Two economically important hosts, cattle and sheep are at high risk from virulent T. annulata and T. lestoquardi, respectively. The survey indicates extensive exposure to ticks and transmission of infection that has a significant economic impact. The higher prevalence of T. lestoquardi as mixed rather than single infection requires further investigation.

Original languageEnglish
JournalTicks and Tick-borne Diseases
DOIs
Publication statusAccepted/In press - Oct 11 2016

Fingerprint

Theileria
Oman
Livestock
Sheep
Parasites
livestock
sheep
parasites
Ovis
monitoring
goats
Theileria annulata
Goats
cattle
ticks
infection
Ticks
mixed infection
Infection
restriction fragment length polymorphism

Keywords

  • Molecular epidemiology
  • Oman
  • Theileria annulata

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Insect Science
  • Infectious Diseases

Cite this

Al-Fahdi, A., Alqamashoui, B., Al-Hamidhi, S., Kose, O., Tageldin, M. H., Bobade, P., ... Babiker, H. (Accepted/In press). Molecular surveillance of Theileria parasites of livestock in Oman. Ticks and Tick-borne Diseases. https://doi.org/10.1016/j.ttbdis.2017.05.008

Molecular surveillance of Theileria parasites of livestock in Oman. / Al-Fahdi, Amira; Alqamashoui, Badar; Al-Hamidhi, Salama; Kose, Onur; Tageldin, Mohammed H.; Bobade, Patrick; Johnson, Eugene H.; Hussain, Abdel Rahim; Karagenc, Tulin; Tait, Andy; Shiels, Brian; Bilgic, Huseyin Bilgin; Babiker, Hamza.

In: Ticks and Tick-borne Diseases, 11.10.2016.

Research output: Contribution to journalArticle

Al-Fahdi, A, Alqamashoui, B, Al-Hamidhi, S, Kose, O, Tageldin, MH, Bobade, P, Johnson, EH, Hussain, AR, Karagenc, T, Tait, A, Shiels, B, Bilgic, HB & Babiker, H 2016, 'Molecular surveillance of Theileria parasites of livestock in Oman', Ticks and Tick-borne Diseases. https://doi.org/10.1016/j.ttbdis.2017.05.008
Al-Fahdi A, Alqamashoui B, Al-Hamidhi S, Kose O, Tageldin MH, Bobade P et al. Molecular surveillance of Theileria parasites of livestock in Oman. Ticks and Tick-borne Diseases. 2016 Oct 11. https://doi.org/10.1016/j.ttbdis.2017.05.008
Al-Fahdi, Amira ; Alqamashoui, Badar ; Al-Hamidhi, Salama ; Kose, Onur ; Tageldin, Mohammed H. ; Bobade, Patrick ; Johnson, Eugene H. ; Hussain, Abdel Rahim ; Karagenc, Tulin ; Tait, Andy ; Shiels, Brian ; Bilgic, Huseyin Bilgin ; Babiker, Hamza. / Molecular surveillance of Theileria parasites of livestock in Oman. In: Ticks and Tick-borne Diseases. 2016.
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title = "Molecular surveillance of Theileria parasites of livestock in Oman",
abstract = "Background: Theileriosis is one of the most prevalent infectious diseases of livestock in the Arabian Peninsula, and causes high rates of mortality and morbidity in sheep and cattle. However, there is a paucity of information on the distribution of Theileria spp. over the whole region and their impact on different hosts. The present study carried out a country-wide molecular survey for Theileria spp. of livestock in Oman across four governorates. The aim of the survey was to define the prevalence of Theileria spp. in cattle, sheep and goats, highlight risk factors for infection and identify the main tick species involved in parasite transmission. Material and methods: A total of 2020 animals were examined in the survey consisting of sheep [n = 592], goats [n = 981] and cattle [n = 447]. All three species were raised and co-grazed on the same farms. Theileria parasites were detected using PCR-RFLP and RLB of the 18S rRNA gene. Cloning and sequencing of the 18S rRNA was carried out on 11 T. lestoquardi isolates from Ash-Sharqiyah, and Ad-Dhahira governorates, and phylogenetic relationships were inferred using additional sequences of T. lestoquardi, T. annulata and T. ovis available in GenBank. Results: Theileria spp. prevalence was 72.3{\%}, 36.7{\%} and 2.7{\%} among cattle, sheep and goats, respectively. Strong similarity in results was obtained using RLB and PCR-RFLP for detection of Theileria spp. however, RLB detected a higher rate of mixed infection than PCR-RFPL (P. <. 0.001). Theileria annulata was the only parasite detected in cattle, while sheep and goats carried T. ovis, T. lestoquardi and T. annulata as well as Theileria spp. OT1. Of the four Theileria spp. detected in small ruminants, overall T. ovis was most prevalent (sheep [33.4{\%}], goats [2.0{\%}]), whereas T. lestoquardi was less prevalent (sheep [22.0{\%}], goats [0.5{\%}]). A large proportion of infected sheep (19{\%}) carried mixed infection of T. ovis and T. lestoquardi. However, single T. lestoquardi infections (3.0{\%}) were less prevalent than T. ovis infections (14.5{\%}). Risk of Theileria spp. infection was significantly higher for exotic breeds, relative to native breeds, of cattle (p = 0.00002) and sheep (p = 0.005). Phylogenetic analysis placed T. lestoquardi in Oman in the same clade as other T. lestoquardi strains isolated from the same regional area (Iraq and Iran). The main tick species, identified on the examined animals. , Hyalomma anatolicum, was widely distributed and was found in all of the surveyed governorates. Conclusion: Theileria spp. are widespread in Oman with variable prevalence detected in different regions. Two economically important hosts, cattle and sheep are at high risk from virulent T. annulata and T. lestoquardi, respectively. The survey indicates extensive exposure to ticks and transmission of infection that has a significant economic impact. The higher prevalence of T. lestoquardi as mixed rather than single infection requires further investigation.",
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author = "Amira Al-Fahdi and Badar Alqamashoui and Salama Al-Hamidhi and Onur Kose and Tageldin, {Mohammed H.} and Patrick Bobade and Johnson, {Eugene H.} and Hussain, {Abdel Rahim} and Tulin Karagenc and Andy Tait and Brian Shiels and Bilgic, {Huseyin Bilgin} and Hamza Babiker",
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T1 - Molecular surveillance of Theileria parasites of livestock in Oman

AU - Al-Fahdi, Amira

AU - Alqamashoui, Badar

AU - Al-Hamidhi, Salama

AU - Kose, Onur

AU - Tageldin, Mohammed H.

AU - Bobade, Patrick

AU - Johnson, Eugene H.

AU - Hussain, Abdel Rahim

AU - Karagenc, Tulin

AU - Tait, Andy

AU - Shiels, Brian

AU - Bilgic, Huseyin Bilgin

AU - Babiker, Hamza

PY - 2016/10/11

Y1 - 2016/10/11

N2 - Background: Theileriosis is one of the most prevalent infectious diseases of livestock in the Arabian Peninsula, and causes high rates of mortality and morbidity in sheep and cattle. However, there is a paucity of information on the distribution of Theileria spp. over the whole region and their impact on different hosts. The present study carried out a country-wide molecular survey for Theileria spp. of livestock in Oman across four governorates. The aim of the survey was to define the prevalence of Theileria spp. in cattle, sheep and goats, highlight risk factors for infection and identify the main tick species involved in parasite transmission. Material and methods: A total of 2020 animals were examined in the survey consisting of sheep [n = 592], goats [n = 981] and cattle [n = 447]. All three species were raised and co-grazed on the same farms. Theileria parasites were detected using PCR-RFLP and RLB of the 18S rRNA gene. Cloning and sequencing of the 18S rRNA was carried out on 11 T. lestoquardi isolates from Ash-Sharqiyah, and Ad-Dhahira governorates, and phylogenetic relationships were inferred using additional sequences of T. lestoquardi, T. annulata and T. ovis available in GenBank. Results: Theileria spp. prevalence was 72.3%, 36.7% and 2.7% among cattle, sheep and goats, respectively. Strong similarity in results was obtained using RLB and PCR-RFLP for detection of Theileria spp. however, RLB detected a higher rate of mixed infection than PCR-RFPL (P. <. 0.001). Theileria annulata was the only parasite detected in cattle, while sheep and goats carried T. ovis, T. lestoquardi and T. annulata as well as Theileria spp. OT1. Of the four Theileria spp. detected in small ruminants, overall T. ovis was most prevalent (sheep [33.4%], goats [2.0%]), whereas T. lestoquardi was less prevalent (sheep [22.0%], goats [0.5%]). A large proportion of infected sheep (19%) carried mixed infection of T. ovis and T. lestoquardi. However, single T. lestoquardi infections (3.0%) were less prevalent than T. ovis infections (14.5%). Risk of Theileria spp. infection was significantly higher for exotic breeds, relative to native breeds, of cattle (p = 0.00002) and sheep (p = 0.005). Phylogenetic analysis placed T. lestoquardi in Oman in the same clade as other T. lestoquardi strains isolated from the same regional area (Iraq and Iran). The main tick species, identified on the examined animals. , Hyalomma anatolicum, was widely distributed and was found in all of the surveyed governorates. Conclusion: Theileria spp. are widespread in Oman with variable prevalence detected in different regions. Two economically important hosts, cattle and sheep are at high risk from virulent T. annulata and T. lestoquardi, respectively. The survey indicates extensive exposure to ticks and transmission of infection that has a significant economic impact. The higher prevalence of T. lestoquardi as mixed rather than single infection requires further investigation.

AB - Background: Theileriosis is one of the most prevalent infectious diseases of livestock in the Arabian Peninsula, and causes high rates of mortality and morbidity in sheep and cattle. However, there is a paucity of information on the distribution of Theileria spp. over the whole region and their impact on different hosts. The present study carried out a country-wide molecular survey for Theileria spp. of livestock in Oman across four governorates. The aim of the survey was to define the prevalence of Theileria spp. in cattle, sheep and goats, highlight risk factors for infection and identify the main tick species involved in parasite transmission. Material and methods: A total of 2020 animals were examined in the survey consisting of sheep [n = 592], goats [n = 981] and cattle [n = 447]. All three species were raised and co-grazed on the same farms. Theileria parasites were detected using PCR-RFLP and RLB of the 18S rRNA gene. Cloning and sequencing of the 18S rRNA was carried out on 11 T. lestoquardi isolates from Ash-Sharqiyah, and Ad-Dhahira governorates, and phylogenetic relationships were inferred using additional sequences of T. lestoquardi, T. annulata and T. ovis available in GenBank. Results: Theileria spp. prevalence was 72.3%, 36.7% and 2.7% among cattle, sheep and goats, respectively. Strong similarity in results was obtained using RLB and PCR-RFLP for detection of Theileria spp. however, RLB detected a higher rate of mixed infection than PCR-RFPL (P. <. 0.001). Theileria annulata was the only parasite detected in cattle, while sheep and goats carried T. ovis, T. lestoquardi and T. annulata as well as Theileria spp. OT1. Of the four Theileria spp. detected in small ruminants, overall T. ovis was most prevalent (sheep [33.4%], goats [2.0%]), whereas T. lestoquardi was less prevalent (sheep [22.0%], goats [0.5%]). A large proportion of infected sheep (19%) carried mixed infection of T. ovis and T. lestoquardi. However, single T. lestoquardi infections (3.0%) were less prevalent than T. ovis infections (14.5%). Risk of Theileria spp. infection was significantly higher for exotic breeds, relative to native breeds, of cattle (p = 0.00002) and sheep (p = 0.005). Phylogenetic analysis placed T. lestoquardi in Oman in the same clade as other T. lestoquardi strains isolated from the same regional area (Iraq and Iran). The main tick species, identified on the examined animals. , Hyalomma anatolicum, was widely distributed and was found in all of the surveyed governorates. Conclusion: Theileria spp. are widespread in Oman with variable prevalence detected in different regions. Two economically important hosts, cattle and sheep are at high risk from virulent T. annulata and T. lestoquardi, respectively. The survey indicates extensive exposure to ticks and transmission of infection that has a significant economic impact. The higher prevalence of T. lestoquardi as mixed rather than single infection requires further investigation.

KW - Molecular epidemiology

KW - Oman

KW - Theileria annulata

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