TY - JOUR
T1 - Molecular characterization of the African orthobunyavirus Ilesha virus
AU - Pachler, Karin
AU - Růžek, Daniel
AU - Nowotny, Norbert
N1 - Funding Information:
We are grateful to Dr. Jolanta Kolodziejek for her excellent support in preparing the phylogenetic trees and to Dr. Vlasta Danielová for her help in obtaining the virus strain. This study was partially funded by the EU grant FP7-261504 EDENext and is catalogued by the EDENext Steering Committee as EDENext168 ( http://www.edenext.eu ). The contents of this publication are the sole responsibility of the authors and don’t necessarily reflect the views of the European Commission.
Funding Information:
DR was supported by the Czech Science Foundation project no. P502/11/2116 , and AdmireVet project no. CZ.1.05./2.1.00/01.006 (ED006/01/01) . The founders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
PY - 2013/12
Y1 - 2013/12
N2 - Ilesha virus is an arthropod-borne virus belonging to the genus Orthobunyavirus of the Bunyaviridae family. Ilesha virus has been isolated from humans in several African countries, mostly in relation with febrile illness and erythema, though there are reported cases of fatal meningoencephalitis and hemorrhagic fever.In the present study, we report the complete genomic sequence of all three Ilesha virus segments (S, M, L) and characterize the open reading frames. The nucleoprotein encoded by segment S contains 59 conserved orthobunyavirus amino acids putatively critical for protein function. For the polyprotein encoded by segment M, potential proteolytic cleavage sites and N-glycosylation sites as well as conserved cysteines are described in reference to other orthobunyaviruses. Within the C terminal glycoprotein Gc a putative fusion peptide could be localized. In the RNA-dependent RNA polymerase encoded by segment L, all strictly conserved amino acids within the four conserved regions known to be catalytically active are present.Phylogenetic analyses conducted for each Ilesha virus genomic segment confirm the classification of Ilesha virus within the Bunyamwera serogroup of orthobunyaviruses. Ilesha virus segments S and L exhibit highest genetic conservation with Bunyamwera virus and Ngari virus, with maximum sequence identities of 88% for segment S and 82% for segment L. However, the M segment was found to be more diverse with a maximum nucleotide identity of 72% to Bunyamwera serogroup viruses.
AB - Ilesha virus is an arthropod-borne virus belonging to the genus Orthobunyavirus of the Bunyaviridae family. Ilesha virus has been isolated from humans in several African countries, mostly in relation with febrile illness and erythema, though there are reported cases of fatal meningoencephalitis and hemorrhagic fever.In the present study, we report the complete genomic sequence of all three Ilesha virus segments (S, M, L) and characterize the open reading frames. The nucleoprotein encoded by segment S contains 59 conserved orthobunyavirus amino acids putatively critical for protein function. For the polyprotein encoded by segment M, potential proteolytic cleavage sites and N-glycosylation sites as well as conserved cysteines are described in reference to other orthobunyaviruses. Within the C terminal glycoprotein Gc a putative fusion peptide could be localized. In the RNA-dependent RNA polymerase encoded by segment L, all strictly conserved amino acids within the four conserved regions known to be catalytically active are present.Phylogenetic analyses conducted for each Ilesha virus genomic segment confirm the classification of Ilesha virus within the Bunyamwera serogroup of orthobunyaviruses. Ilesha virus segments S and L exhibit highest genetic conservation with Bunyamwera virus and Ngari virus, with maximum sequence identities of 88% for segment S and 82% for segment L. However, the M segment was found to be more diverse with a maximum nucleotide identity of 72% to Bunyamwera serogroup viruses.
KW - Genome characterization
KW - Ilesha virus
KW - Orthobunyavirus
KW - Phylogenetic analysis
UR - http://www.scopus.com/inward/record.url?scp=84884191595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884191595&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2013.08.015
DO - 10.1016/j.meegid.2013.08.015
M3 - Article
C2 - 23988729
AN - SCOPUS:84884191595
SN - 1567-1348
VL - 20
SP - 124
EP - 130
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -