TY - JOUR
T1 - Major histocompatibility complex class II antigen expression in rejecting cardiac allografts
T2 - Detection using in vivo imaging with radiolabeled monoclonal antibody
AU - McGhie, A. Iain
AU - Radovancevic, Branislav
AU - Capek, Pavel
AU - Moore, Warren H.
AU - Kasi, Leela
AU - Lamki, Lamk
AU - Clubb, Fred J.
AU - Frazier, O. Howard
AU - Willerson, James T.
PY - 1997/9/2
Y1 - 1997/9/2
N2 - Background: Increased expression of major histocompatibility complex class II (MHC-II) antigen occurs during cardiac allograft rejection. We tested the hypotheses that (1) radiolabeled antibody to MHC-II antigen allows detection of cardiac allograft rejection using nuclear imaging techniques and (2) uptake of radiolabeled antibody to MHC-II antigen correlates with severity of rejection. Methods and Results: Thirteen beagles with cervical cardiac allografts were studied for 64±23 days by use of myocardial biopsy and in vivo imaging. Uptake of radiolabeled (131I[n=2], 123I[n=1], or 111In[n=10]) antibody to MHC-II increased over baseline in 7 animals that developed histological evidence of progressively worsening allograft rejection (group A), from 72.2±46.1 to 176.8±102.0 counts/pixel/mCi (P<.009). In 4 beagles without progressively worsening allograft rejection (group B), uptake was unchanged during follow-up (74.4±43.8 and 60.2±37.4 counts/pixel/mCi; P=NS). In animals studied with 111In-labeled antibody, uptake increased from 102.9±23.1 at baseline to 233.2±82.7 counts/pixel/mCi at follow-up in group A animals (P=.036), with no significant change in group B (91.1±34.9 and 75.9±24.9 counts/pixel/mCi; P=NS). Uptake of 111In- labeled antibody was 107.5±35.7, 135.9±70.8, and 307.8±90.1 counts/pixel/mCi in biopsy samples showing evidence of mild, moderate, and severe rejection, respectively (P=.001). Biopsy samples showing mild, moderate, and intense MHC-II expression antibody uptake had uptakes of 92.6±36.3, 158.5±54.7, and 307.8±90.1 counts/pixel/mCi, respectively (P=.00004). Conclusions: Radiolabeled monoclonal antibodies to MHC-II antigen can detect cardiac allograft rejection in this large mammal model of cardiac allograft transplantation, and this technique may have a potential role in the detection of rejection in patients after cardiac transplantation.
AB - Background: Increased expression of major histocompatibility complex class II (MHC-II) antigen occurs during cardiac allograft rejection. We tested the hypotheses that (1) radiolabeled antibody to MHC-II antigen allows detection of cardiac allograft rejection using nuclear imaging techniques and (2) uptake of radiolabeled antibody to MHC-II antigen correlates with severity of rejection. Methods and Results: Thirteen beagles with cervical cardiac allografts were studied for 64±23 days by use of myocardial biopsy and in vivo imaging. Uptake of radiolabeled (131I[n=2], 123I[n=1], or 111In[n=10]) antibody to MHC-II increased over baseline in 7 animals that developed histological evidence of progressively worsening allograft rejection (group A), from 72.2±46.1 to 176.8±102.0 counts/pixel/mCi (P<.009). In 4 beagles without progressively worsening allograft rejection (group B), uptake was unchanged during follow-up (74.4±43.8 and 60.2±37.4 counts/pixel/mCi; P=NS). In animals studied with 111In-labeled antibody, uptake increased from 102.9±23.1 at baseline to 233.2±82.7 counts/pixel/mCi at follow-up in group A animals (P=.036), with no significant change in group B (91.1±34.9 and 75.9±24.9 counts/pixel/mCi; P=NS). Uptake of 111In- labeled antibody was 107.5±35.7, 135.9±70.8, and 307.8±90.1 counts/pixel/mCi in biopsy samples showing evidence of mild, moderate, and severe rejection, respectively (P=.001). Biopsy samples showing mild, moderate, and intense MHC-II expression antibody uptake had uptakes of 92.6±36.3, 158.5±54.7, and 307.8±90.1 counts/pixel/mCi, respectively (P=.00004). Conclusions: Radiolabeled monoclonal antibodies to MHC-II antigen can detect cardiac allograft rejection in this large mammal model of cardiac allograft transplantation, and this technique may have a potential role in the detection of rejection in patients after cardiac transplantation.
KW - Imaging
KW - Immunology
KW - Radioisotopes
KW - Rejection
KW - Transplantation
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U2 - 10.1161/01.CIR.96.5.1605
DO - 10.1161/01.CIR.96.5.1605
M3 - Article
C2 - 9315554
AN - SCOPUS:0030776738
SN - 0009-7322
VL - 96
SP - 1605
EP - 1611
JO - Circulation
JF - Circulation
IS - 5
ER -