Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008

H. Foo, C. C. Blyth, S. van Hal, K. McPhie, M. Ratnamohan, M. Fennell, F. Ba Alawi, W. Rawlinson, S. Adamson, P. Armstrong, D. E. Dwyer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: The performance of influenza laboratory diagnostics in young adults and in the setting of outbreaks during mass gatherings has not been well studied. Objectives: We compare the performance of point-of-care tests (POCTs) and immunofluorescence assays (IFAs) with nucleic acid tests (NATs) and viral culture in pilgrims attending influenza clinics established during a large influenza outbreak (World Youth Day, Sydney, Australia, 2008) to assess their performance under the real-life pressures of a mass influenza outbreak. Study design: Patients with an influenza-like illness (ILI) underwent respiratory specimen sampling. Combined deep nares and throat swabs were collected for POCT by trained or untrained clinic staff; type-specific IFA; NAT and viral culture. Laboratory-confirmed influenza occurred if viral culture and/or NAT were positive; the performance of laboratory tests was calculated against this 'gold standard'. Results: A total of 230 samples were collected from 227 patients (median age, 20 years; interquartile range, 18-28 years), with 95 samples (41.3%) having laboratory-confirmed influenza infection (influenza A, 57; influenza B, 38). IFA and POCT sensitivities were 74.5% and 55%, respectively. Four of 51 (8%) culture-positive specimens were negative by NAT, and several errors in influenza virus typing occurred with IFA, POCT and NAT. A non-significant trend towards better POCT performance with increased operator training was demonstrated. Conclusion: Different environments, patient populations, operator experience, laboratory access and practicalities associated with performing tests during mass influenza outbreaks may affect performance of influenza-specific laboratory tests.

Original languageEnglish
Pages (from-to)384-386
Number of pages3
JournalJournal of Clinical Virology
Volume46
Issue number4
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Human Influenza
Disease Outbreaks
Young Adult
Point-of-Care Systems
Nucleic Acids
Fluorescent Antibody Technique
Pharynx
Orthomyxoviridae
Pressure
Infection

Keywords

  • Diagnosis
  • Immunofluorescence
  • Influenza
  • Nucleic acid test
  • Outbreak
  • Point-of-care

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Foo, H., Blyth, C. C., van Hal, S., McPhie, K., Ratnamohan, M., Fennell, M., ... Dwyer, D. E. (2009). Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008. Journal of Clinical Virology, 46(4), 384-386. https://doi.org/10.1016/j.jcv.2009.09.019

Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008. / Foo, H.; Blyth, C. C.; van Hal, S.; McPhie, K.; Ratnamohan, M.; Fennell, M.; Ba Alawi, F.; Rawlinson, W.; Adamson, S.; Armstrong, P.; Dwyer, D. E.

In: Journal of Clinical Virology, Vol. 46, No. 4, 12.2009, p. 384-386.

Research output: Contribution to journalArticle

Foo, H, Blyth, CC, van Hal, S, McPhie, K, Ratnamohan, M, Fennell, M, Ba Alawi, F, Rawlinson, W, Adamson, S, Armstrong, P & Dwyer, DE 2009, 'Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008', Journal of Clinical Virology, vol. 46, no. 4, pp. 384-386. https://doi.org/10.1016/j.jcv.2009.09.019
Foo, H. ; Blyth, C. C. ; van Hal, S. ; McPhie, K. ; Ratnamohan, M. ; Fennell, M. ; Ba Alawi, F. ; Rawlinson, W. ; Adamson, S. ; Armstrong, P. ; Dwyer, D. E. / Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008. In: Journal of Clinical Virology. 2009 ; Vol. 46, No. 4. pp. 384-386.
@article{71688e18dfd8427e97972945ba51d71b,
title = "Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008",
abstract = "Background: The performance of influenza laboratory diagnostics in young adults and in the setting of outbreaks during mass gatherings has not been well studied. Objectives: We compare the performance of point-of-care tests (POCTs) and immunofluorescence assays (IFAs) with nucleic acid tests (NATs) and viral culture in pilgrims attending influenza clinics established during a large influenza outbreak (World Youth Day, Sydney, Australia, 2008) to assess their performance under the real-life pressures of a mass influenza outbreak. Study design: Patients with an influenza-like illness (ILI) underwent respiratory specimen sampling. Combined deep nares and throat swabs were collected for POCT by trained or untrained clinic staff; type-specific IFA; NAT and viral culture. Laboratory-confirmed influenza occurred if viral culture and/or NAT were positive; the performance of laboratory tests was calculated against this 'gold standard'. Results: A total of 230 samples were collected from 227 patients (median age, 20 years; interquartile range, 18-28 years), with 95 samples (41.3{\%}) having laboratory-confirmed influenza infection (influenza A, 57; influenza B, 38). IFA and POCT sensitivities were 74.5{\%} and 55{\%}, respectively. Four of 51 (8{\%}) culture-positive specimens were negative by NAT, and several errors in influenza virus typing occurred with IFA, POCT and NAT. A non-significant trend towards better POCT performance with increased operator training was demonstrated. Conclusion: Different environments, patient populations, operator experience, laboratory access and practicalities associated with performing tests during mass influenza outbreaks may affect performance of influenza-specific laboratory tests.",
keywords = "Diagnosis, Immunofluorescence, Influenza, Nucleic acid test, Outbreak, Point-of-care",
author = "H. Foo and Blyth, {C. C.} and {van Hal}, S. and K. McPhie and M. Ratnamohan and M. Fennell and {Ba Alawi}, F. and W. Rawlinson and S. Adamson and P. Armstrong and Dwyer, {D. E.}",
year = "2009",
month = "12",
doi = "10.1016/j.jcv.2009.09.019",
language = "English",
volume = "46",
pages = "384--386",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Laboratory test performance in young adults during influenza outbreaks at World Youth Day 2008

AU - Foo, H.

AU - Blyth, C. C.

AU - van Hal, S.

AU - McPhie, K.

AU - Ratnamohan, M.

AU - Fennell, M.

AU - Ba Alawi, F.

AU - Rawlinson, W.

AU - Adamson, S.

AU - Armstrong, P.

AU - Dwyer, D. E.

PY - 2009/12

Y1 - 2009/12

N2 - Background: The performance of influenza laboratory diagnostics in young adults and in the setting of outbreaks during mass gatherings has not been well studied. Objectives: We compare the performance of point-of-care tests (POCTs) and immunofluorescence assays (IFAs) with nucleic acid tests (NATs) and viral culture in pilgrims attending influenza clinics established during a large influenza outbreak (World Youth Day, Sydney, Australia, 2008) to assess their performance under the real-life pressures of a mass influenza outbreak. Study design: Patients with an influenza-like illness (ILI) underwent respiratory specimen sampling. Combined deep nares and throat swabs were collected for POCT by trained or untrained clinic staff; type-specific IFA; NAT and viral culture. Laboratory-confirmed influenza occurred if viral culture and/or NAT were positive; the performance of laboratory tests was calculated against this 'gold standard'. Results: A total of 230 samples were collected from 227 patients (median age, 20 years; interquartile range, 18-28 years), with 95 samples (41.3%) having laboratory-confirmed influenza infection (influenza A, 57; influenza B, 38). IFA and POCT sensitivities were 74.5% and 55%, respectively. Four of 51 (8%) culture-positive specimens were negative by NAT, and several errors in influenza virus typing occurred with IFA, POCT and NAT. A non-significant trend towards better POCT performance with increased operator training was demonstrated. Conclusion: Different environments, patient populations, operator experience, laboratory access and practicalities associated with performing tests during mass influenza outbreaks may affect performance of influenza-specific laboratory tests.

AB - Background: The performance of influenza laboratory diagnostics in young adults and in the setting of outbreaks during mass gatherings has not been well studied. Objectives: We compare the performance of point-of-care tests (POCTs) and immunofluorescence assays (IFAs) with nucleic acid tests (NATs) and viral culture in pilgrims attending influenza clinics established during a large influenza outbreak (World Youth Day, Sydney, Australia, 2008) to assess their performance under the real-life pressures of a mass influenza outbreak. Study design: Patients with an influenza-like illness (ILI) underwent respiratory specimen sampling. Combined deep nares and throat swabs were collected for POCT by trained or untrained clinic staff; type-specific IFA; NAT and viral culture. Laboratory-confirmed influenza occurred if viral culture and/or NAT were positive; the performance of laboratory tests was calculated against this 'gold standard'. Results: A total of 230 samples were collected from 227 patients (median age, 20 years; interquartile range, 18-28 years), with 95 samples (41.3%) having laboratory-confirmed influenza infection (influenza A, 57; influenza B, 38). IFA and POCT sensitivities were 74.5% and 55%, respectively. Four of 51 (8%) culture-positive specimens were negative by NAT, and several errors in influenza virus typing occurred with IFA, POCT and NAT. A non-significant trend towards better POCT performance with increased operator training was demonstrated. Conclusion: Different environments, patient populations, operator experience, laboratory access and practicalities associated with performing tests during mass influenza outbreaks may affect performance of influenza-specific laboratory tests.

KW - Diagnosis

KW - Immunofluorescence

KW - Influenza

KW - Nucleic acid test

KW - Outbreak

KW - Point-of-care

UR - http://www.scopus.com/inward/record.url?scp=71549141311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71549141311&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2009.09.019

DO - 10.1016/j.jcv.2009.09.019

M3 - Article

VL - 46

SP - 384

EP - 386

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

IS - 4

ER -