Influence of different cultivars of Phoenix dactylifera L-date fruits on blood clotting and wound healing

Sidgi Syed Anwar Hasson, M. S. Al-Shaqsi, J. Z. Albusaidi, Mohammad Al-Balushi, F. L. Hakkim, G. M. Aleemallah, A. A. Al-Jabri

Research output: Contribution to journalArticle

Abstract

Objective: To investigate different types of dates and medical properties of influencing blood clotting and wound healing in an animal model. Methods: Three different cultivars of dates (Ajwa, Khalas, and Fardh) were examined in-vivo, for blood clotting and wound healing using CD1 mice of both sexes. Study of toxicity to animals was performed accordingly prior to further investigations. The ethanolic extracts were given orally to animals as a constituent in their daily water. Blood samples were obtained from the mice inferior vena cava to carry out the prothrombin time (PT) assay using the manual method and confirmed using a semi-automated machine. The bleeding time (BT) assay was performed using the cutting technique. In the wound healing analysis, a small cut (5-10 mm) in the skin overlying the thigh was conducted in all mice under anesthesia. The diameter of the cut and healing status were measured on a daily basis throughout the time of the experiment using a roller. Results: Ajwa was able to elevate both PT and BT (P<0.05), significantly in a time-dependent manner followed by Khalas date (P<0.05). The results of PT and BT of Fardh date were found to be very close to those of the control group (P<0.05). Despite its activity as an anticoagulant, Khalas date showed a potential property to enhance wound healing in contrast to other dates and the control groups in this study. Conclusions: Omani Khalas date fruit has both antithrombotic as well as wound healing properties. The results open a new gate with these fruits for exploring the potential component(s) that may play an important role in antithrombotic as well as wound healing process.

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalAsian Pacific Journal of Tropical Biomedicine
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 1 2018

Fingerprint

Prothrombin
Blood Coagulation
Fruits
Wound Healing
Animals
Blood
Bleeding Time
Assays
Prothrombin Time
Anticoagulants
Toxicity
Skin
Control Groups
Water
Inferior Vena Cava
Thigh
Phoeniceae
Fruit
Anesthesia
Animal Models

Keywords

  • Bleeding Time
  • Phoenix dactylifera L
  • Prothrombin Time
  • Wound Healing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Influence of different cultivars of Phoenix dactylifera L-date fruits on blood clotting and wound healing. / Syed Anwar Hasson, Sidgi; Al-Shaqsi, M. S.; Albusaidi, J. Z.; Al-Balushi, Mohammad; Hakkim, F. L.; Aleemallah, G. M.; Al-Jabri, A. A.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 8, No. 7, 01.07.2018, p. 371-376.

Research output: Contribution to journalArticle

Syed Anwar Hasson, Sidgi ; Al-Shaqsi, M. S. ; Albusaidi, J. Z. ; Al-Balushi, Mohammad ; Hakkim, F. L. ; Aleemallah, G. M. ; Al-Jabri, A. A. / Influence of different cultivars of Phoenix dactylifera L-date fruits on blood clotting and wound healing. In: Asian Pacific Journal of Tropical Biomedicine. 2018 ; Vol. 8, No. 7. pp. 371-376.
@article{971b6d3b31bd46f08671854cab447515,
title = "Influence of different cultivars of Phoenix dactylifera L-date fruits on blood clotting and wound healing",
abstract = "Objective: To investigate different types of dates and medical properties of influencing blood clotting and wound healing in an animal model. Methods: Three different cultivars of dates (Ajwa, Khalas, and Fardh) were examined in-vivo, for blood clotting and wound healing using CD1 mice of both sexes. Study of toxicity to animals was performed accordingly prior to further investigations. The ethanolic extracts were given orally to animals as a constituent in their daily water. Blood samples were obtained from the mice inferior vena cava to carry out the prothrombin time (PT) assay using the manual method and confirmed using a semi-automated machine. The bleeding time (BT) assay was performed using the cutting technique. In the wound healing analysis, a small cut (5-10 mm) in the skin overlying the thigh was conducted in all mice under anesthesia. The diameter of the cut and healing status were measured on a daily basis throughout the time of the experiment using a roller. Results: Ajwa was able to elevate both PT and BT (P<0.05), significantly in a time-dependent manner followed by Khalas date (P<0.05). The results of PT and BT of Fardh date were found to be very close to those of the control group (P<0.05). Despite its activity as an anticoagulant, Khalas date showed a potential property to enhance wound healing in contrast to other dates and the control groups in this study. Conclusions: Omani Khalas date fruit has both antithrombotic as well as wound healing properties. The results open a new gate with these fruits for exploring the potential component(s) that may play an important role in antithrombotic as well as wound healing process.",
keywords = "Bleeding Time, Phoenix dactylifera L, Prothrombin Time, Wound Healing",
author = "{Syed Anwar Hasson}, Sidgi and Al-Shaqsi, {M. S.} and Albusaidi, {J. Z.} and Mohammad Al-Balushi and Hakkim, {F. L.} and Aleemallah, {G. M.} and Al-Jabri, {A. A.}",
year = "2018",
month = "7",
day = "1",
doi = "10.4103/2221-1691.237081",
language = "English",
volume = "8",
pages = "371--376",
journal = "Asian Pacific Journal of Tropical Biomedicine",
issn = "2221-1691",
publisher = "Elsevier BV",
number = "7",

}

TY - JOUR

T1 - Influence of different cultivars of Phoenix dactylifera L-date fruits on blood clotting and wound healing

AU - Syed Anwar Hasson, Sidgi

AU - Al-Shaqsi, M. S.

AU - Albusaidi, J. Z.

AU - Al-Balushi, Mohammad

AU - Hakkim, F. L.

AU - Aleemallah, G. M.

AU - Al-Jabri, A. A.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Objective: To investigate different types of dates and medical properties of influencing blood clotting and wound healing in an animal model. Methods: Three different cultivars of dates (Ajwa, Khalas, and Fardh) were examined in-vivo, for blood clotting and wound healing using CD1 mice of both sexes. Study of toxicity to animals was performed accordingly prior to further investigations. The ethanolic extracts were given orally to animals as a constituent in their daily water. Blood samples were obtained from the mice inferior vena cava to carry out the prothrombin time (PT) assay using the manual method and confirmed using a semi-automated machine. The bleeding time (BT) assay was performed using the cutting technique. In the wound healing analysis, a small cut (5-10 mm) in the skin overlying the thigh was conducted in all mice under anesthesia. The diameter of the cut and healing status were measured on a daily basis throughout the time of the experiment using a roller. Results: Ajwa was able to elevate both PT and BT (P<0.05), significantly in a time-dependent manner followed by Khalas date (P<0.05). The results of PT and BT of Fardh date were found to be very close to those of the control group (P<0.05). Despite its activity as an anticoagulant, Khalas date showed a potential property to enhance wound healing in contrast to other dates and the control groups in this study. Conclusions: Omani Khalas date fruit has both antithrombotic as well as wound healing properties. The results open a new gate with these fruits for exploring the potential component(s) that may play an important role in antithrombotic as well as wound healing process.

AB - Objective: To investigate different types of dates and medical properties of influencing blood clotting and wound healing in an animal model. Methods: Three different cultivars of dates (Ajwa, Khalas, and Fardh) were examined in-vivo, for blood clotting and wound healing using CD1 mice of both sexes. Study of toxicity to animals was performed accordingly prior to further investigations. The ethanolic extracts were given orally to animals as a constituent in their daily water. Blood samples were obtained from the mice inferior vena cava to carry out the prothrombin time (PT) assay using the manual method and confirmed using a semi-automated machine. The bleeding time (BT) assay was performed using the cutting technique. In the wound healing analysis, a small cut (5-10 mm) in the skin overlying the thigh was conducted in all mice under anesthesia. The diameter of the cut and healing status were measured on a daily basis throughout the time of the experiment using a roller. Results: Ajwa was able to elevate both PT and BT (P<0.05), significantly in a time-dependent manner followed by Khalas date (P<0.05). The results of PT and BT of Fardh date were found to be very close to those of the control group (P<0.05). Despite its activity as an anticoagulant, Khalas date showed a potential property to enhance wound healing in contrast to other dates and the control groups in this study. Conclusions: Omani Khalas date fruit has both antithrombotic as well as wound healing properties. The results open a new gate with these fruits for exploring the potential component(s) that may play an important role in antithrombotic as well as wound healing process.

KW - Bleeding Time

KW - Phoenix dactylifera L

KW - Prothrombin Time

KW - Wound Healing

UR - http://www.scopus.com/inward/record.url?scp=85055037147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055037147&partnerID=8YFLogxK

U2 - 10.4103/2221-1691.237081

DO - 10.4103/2221-1691.237081

M3 - Article

VL - 8

SP - 371

EP - 376

JO - Asian Pacific Journal of Tropical Biomedicine

JF - Asian Pacific Journal of Tropical Biomedicine

SN - 2221-1691

IS - 7

ER -