Induction of active oxygen species (AOS), lipoxygenase and lipid peroxidation in suspension-cultured sugarcane cells by a glycoprotein elicitor isolated from Colletotrichum falcatum

A. Ramesh Sundar, R. Velazhahan, R. Viswanathan, P. Vidhyasekaran

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect of an elicitor isolated from the mycelial walls of Colletotrichum falcatum, the red rot pathogen, in suspension-cultured cells of resistant ('BO 91') and susceptible ('CoC 671') sugarcane cultivars was studied. The elicitor rapidly induced the generation of H2O2 and O2 - within 10 min after treatment in suspension-cultured cells of both resistant and susceptible cultivars. However, the generation of active oxygen species (AOS) was more rapid in suspension-cultured cells of resistant cultivar compared to that of the susceptible one. The level of lipid peroxidation in cell suspension of the resistant cultivar significantly increased 6 h after of elicitor treatment, whereas it increased only 12 h after treatment in the cells of the susceptible cultivar. Lipoxygenase (LOX) activity also significantly increased by 4 h after elicitor treatment in cultured cells of both resistant and susceptible cultivars. However, the increase in LOX activity in suspension cells of the resistant cultivar was greater compared to that of the susceptible one. Suspension-cultured cells of the susceptible cultivar constitutively expressed higher levels of superoxide dismutase (SOD) activity compared to that of the resistant cultivar. Elicitor treatment caused an increase in SOD activity in the susceptible cultivar, whereas in the resistant cultivar, it caused a decrease in SOD activity.

Original languageEnglish
Pages (from-to)441-451
Number of pages11
JournalZeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Volume109
Issue number5
Publication statusPublished - Sep 2002

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Glomerella tucumanensis
lipoxygenase
sugarcane
glycoproteins
lipid peroxidation
cultivars
cultured cells
cells
superoxide dismutase
cell suspension culture
elicitors
active oxygen species

Keywords

  • Active oxygen species
  • Colletotrichum falcatum
  • Sugarcane
  • Suspension-culture

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Induction of active oxygen species (AOS), lipoxygenase and lipid peroxidation in suspension-cultured sugarcane cells by a glycoprotein elicitor isolated from Colletotrichum falcatum",
abstract = "The effect of an elicitor isolated from the mycelial walls of Colletotrichum falcatum, the red rot pathogen, in suspension-cultured cells of resistant ('BO 91') and susceptible ('CoC 671') sugarcane cultivars was studied. The elicitor rapidly induced the generation of H2O2 and O2 - within 10 min after treatment in suspension-cultured cells of both resistant and susceptible cultivars. However, the generation of active oxygen species (AOS) was more rapid in suspension-cultured cells of resistant cultivar compared to that of the susceptible one. The level of lipid peroxidation in cell suspension of the resistant cultivar significantly increased 6 h after of elicitor treatment, whereas it increased only 12 h after treatment in the cells of the susceptible cultivar. Lipoxygenase (LOX) activity also significantly increased by 4 h after elicitor treatment in cultured cells of both resistant and susceptible cultivars. However, the increase in LOX activity in suspension cells of the resistant cultivar was greater compared to that of the susceptible one. Suspension-cultured cells of the susceptible cultivar constitutively expressed higher levels of superoxide dismutase (SOD) activity compared to that of the resistant cultivar. Elicitor treatment caused an increase in SOD activity in the susceptible cultivar, whereas in the resistant cultivar, it caused a decrease in SOD activity.",
keywords = "Active oxygen species, Colletotrichum falcatum, Sugarcane, Suspension-culture",
author = "{Ramesh Sundar}, A. and R. Velazhahan and R. Viswanathan and P. Vidhyasekaran",
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T1 - Induction of active oxygen species (AOS), lipoxygenase and lipid peroxidation in suspension-cultured sugarcane cells by a glycoprotein elicitor isolated from Colletotrichum falcatum

AU - Ramesh Sundar, A.

AU - Velazhahan, R.

AU - Viswanathan, R.

AU - Vidhyasekaran, P.

PY - 2002/9

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N2 - The effect of an elicitor isolated from the mycelial walls of Colletotrichum falcatum, the red rot pathogen, in suspension-cultured cells of resistant ('BO 91') and susceptible ('CoC 671') sugarcane cultivars was studied. The elicitor rapidly induced the generation of H2O2 and O2 - within 10 min after treatment in suspension-cultured cells of both resistant and susceptible cultivars. However, the generation of active oxygen species (AOS) was more rapid in suspension-cultured cells of resistant cultivar compared to that of the susceptible one. The level of lipid peroxidation in cell suspension of the resistant cultivar significantly increased 6 h after of elicitor treatment, whereas it increased only 12 h after treatment in the cells of the susceptible cultivar. Lipoxygenase (LOX) activity also significantly increased by 4 h after elicitor treatment in cultured cells of both resistant and susceptible cultivars. However, the increase in LOX activity in suspension cells of the resistant cultivar was greater compared to that of the susceptible one. Suspension-cultured cells of the susceptible cultivar constitutively expressed higher levels of superoxide dismutase (SOD) activity compared to that of the resistant cultivar. Elicitor treatment caused an increase in SOD activity in the susceptible cultivar, whereas in the resistant cultivar, it caused a decrease in SOD activity.

AB - The effect of an elicitor isolated from the mycelial walls of Colletotrichum falcatum, the red rot pathogen, in suspension-cultured cells of resistant ('BO 91') and susceptible ('CoC 671') sugarcane cultivars was studied. The elicitor rapidly induced the generation of H2O2 and O2 - within 10 min after treatment in suspension-cultured cells of both resistant and susceptible cultivars. However, the generation of active oxygen species (AOS) was more rapid in suspension-cultured cells of resistant cultivar compared to that of the susceptible one. The level of lipid peroxidation in cell suspension of the resistant cultivar significantly increased 6 h after of elicitor treatment, whereas it increased only 12 h after treatment in the cells of the susceptible cultivar. Lipoxygenase (LOX) activity also significantly increased by 4 h after elicitor treatment in cultured cells of both resistant and susceptible cultivars. However, the increase in LOX activity in suspension cells of the resistant cultivar was greater compared to that of the susceptible one. Suspension-cultured cells of the susceptible cultivar constitutively expressed higher levels of superoxide dismutase (SOD) activity compared to that of the resistant cultivar. Elicitor treatment caused an increase in SOD activity in the susceptible cultivar, whereas in the resistant cultivar, it caused a decrease in SOD activity.

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