Incidence of seed-borne fungi and aflatoxins in sudanese lentil seeds

S. A F El-Nagerabi, A. E. Elshafie

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Thirteen seed samples of lentil (Lens esculenta) were incubated on agar plate and moist filter papers (Moist Chambers) at 28 ± 2 °C for determination of the incidence of seed-borne fungi. Aflatoxins content of the seeds was measured using the bright greenish- yellow fluorescence test (BGYF) and thin-layer chromatography (TLC). Sixty-nine species and seven varieties, which belong to 24 genera of fungi, were isolated from this crop. Of these fungi, 51 species and two varieties are considered new for this crop, whereas seven genera and 13 species are new to the mycoflora of the Sudan. The genus Aspergillus (13 species and 6 varieties) which comprising 44% of the total colony count was the most prevalent genus followed by Rhizopus (2 species, 19%), Penicillium (6 species) and Fusarium (8 species) (12%), Chaetomium (3 species) and Cladosporium (5 species) (6%), where the 18 genera (1-4 species) showed very low level of incidence (19%). Of the possible pathogens of lentil plants, F. oxysporum the main cause of vascular wilt was recovered from seeds of this crop. Thin layer chromatographic analysis of chloroform extracts of 13 seed samples showed that only one samples was naturally contaminated with aflatoxins B 1, B 2, G 1 and G 2 (14.3 μg/kg).

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalMycopathologia
Volume149
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Aflatoxins
  • Fungi
  • Lentil
  • Seed-borne
  • Sudan

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

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