In situ hybridization: Detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples

Research output: Contribution to journalReview article

8 Citations (Scopus)

Abstract

In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.

Original languageEnglish
Pages (from-to)653-661
Number of pages9
JournalExpert Review of Molecular Diagnostics
Volume4
Issue number5
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Paraffin
Nucleic Acids
Formaldehyde
In Situ Hybridization
Nucleic Acid Probes
Human Herpesvirus 4
Gene Expression

Keywords

  • Formalin-fixed
  • In situ hybridization
  • Nucleic acid
  • Paraffin-embedded tissues
  • Virus

ASJC Scopus subject areas

  • Genetics

Cite this

@article{ae650498ad04423ca7251560db22fa78,
title = "In situ hybridization: Detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples",
abstract = "In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.",
keywords = "Formalin-fixed, In situ hybridization, Nucleic acid, Paraffin-embedded tissues, Virus",
author = "Mabruk, {M. J E M F}",
year = "2004",
month = "9",
doi = "10.1586/14737159.4.5.653",
language = "English",
volume = "4",
pages = "653--661",
journal = "Expert Review of Molecular Diagnostics",
issn = "1473-7159",
publisher = "Expert Reviews Ltd.",
number = "5",

}

TY - JOUR

T1 - In situ hybridization

T2 - Detecting viral nucleic acid in formalin-fixed, paraffin-embedded tissue samples

AU - Mabruk, M. J E M F

PY - 2004/9

Y1 - 2004/9

N2 - In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.

AB - In situ hybridization is a method for detecting specific nucleic acid sequences within individual cells. This technique permits visualization of viral nucleic acid or gene expression in individual cells within their histologic context. In situ hybridization is based on the complementary binding of a labeled nucleic acid probe to complementary sequences in cells or tissue sections, followed by visualization of target sequences within the cells. It has been used widely for the detection of viral nucleic acid sequences within individual cells. This review will define the technical approaches of in situ hybridization and its current application to detect viral nucleic acids within formalin-fixed, paraffin-embedded tissue samples, with special reference to the Epstein-Barr virus.

KW - Formalin-fixed

KW - In situ hybridization

KW - Nucleic acid

KW - Paraffin-embedded tissues

KW - Virus

UR - http://www.scopus.com/inward/record.url?scp=4544260455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4544260455&partnerID=8YFLogxK

U2 - 10.1586/14737159.4.5.653

DO - 10.1586/14737159.4.5.653

M3 - Review article

C2 - 15347259

AN - SCOPUS:4544260455

VL - 4

SP - 653

EP - 661

JO - Expert Review of Molecular Diagnostics

JF - Expert Review of Molecular Diagnostics

SN - 1473-7159

IS - 5

ER -