Impact of genetic complexity on longevity and gametocytogenesis of Plasmodium falciparum during the dry and transmission-free season of eastern Sudan

Elkhansaa Nassir, Abdel Muhsin A Abdel-Muhsin, Suad Suliaman, Fiona Kenyon, Amani Kheir, Haider Geha, Heather M. Ferguson, David Walliker, Hamza A. Babiker

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Malaria in eastern Sudan is characterised by limited seasonal transmission, with the majority of the year remaining transmission-free. Some inhabitants who contract malaria during the transmission season retain long-lasting sub-patent infections, which probably initiate transmission the following year. Here we have monitored Plasmodium falciparum infection prevalence and gametocyte production during the dry season, and examined the impact of parasite genetic multiplicity on infection longevity. A cohort of 38 individuals who were infected with P. falciparum in November 2001 was monitored monthly by microscopy and PCR until December 2002. Reverse transcriptase polymerase chain reaction of the pfg377 gene was used to detect sub-patent gametocytes. In addition, all isolates were examined for msp-2 alleles and the mean number of parasite clones per infection was estimated. We found that a large proportion (40%) of the cohort retained gametocytes throughout the dry season. The majority of patients retained asexual infection for at least 7 months. Genetic multiplicity of P. falciparum significantly influenced longevity of asexual infection and its gametocyte production. Gametocytes from mixed genotype P. falciparum infections persisted three times longer than those from single genotype infections, suggesting that genetic diversity promotes persistence. These findings are discussed in the context of the parasite biology and malaria epidemiology in the study area.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalInternational Journal for Parasitology
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Sudan
Plasmodium falciparum
Malaria
Infection
Parasites
Genotype
Contracts
Reverse Transcriptase Polymerase Chain Reaction
Microscopy
Epidemiology
Clone Cells
Alleles
Polymerase Chain Reaction
Genes

Keywords

  • Gametocytes
  • Plasmodium falciparum
  • RT-PCR
  • Seasonal malaria

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Impact of genetic complexity on longevity and gametocytogenesis of Plasmodium falciparum during the dry and transmission-free season of eastern Sudan. / Nassir, Elkhansaa; Abdel-Muhsin, Abdel Muhsin A; Suliaman, Suad; Kenyon, Fiona; Kheir, Amani; Geha, Haider; Ferguson, Heather M.; Walliker, David; Babiker, Hamza A.

In: International Journal for Parasitology, Vol. 35, No. 1, 01.2005, p. 49-55.

Research output: Contribution to journalArticle

Nassir, Elkhansaa ; Abdel-Muhsin, Abdel Muhsin A ; Suliaman, Suad ; Kenyon, Fiona ; Kheir, Amani ; Geha, Haider ; Ferguson, Heather M. ; Walliker, David ; Babiker, Hamza A. / Impact of genetic complexity on longevity and gametocytogenesis of Plasmodium falciparum during the dry and transmission-free season of eastern Sudan. In: International Journal for Parasitology. 2005 ; Vol. 35, No. 1. pp. 49-55.
@article{b34997254e2d4b51a7bfb2ce0378592e,
title = "Impact of genetic complexity on longevity and gametocytogenesis of Plasmodium falciparum during the dry and transmission-free season of eastern Sudan",
abstract = "Malaria in eastern Sudan is characterised by limited seasonal transmission, with the majority of the year remaining transmission-free. Some inhabitants who contract malaria during the transmission season retain long-lasting sub-patent infections, which probably initiate transmission the following year. Here we have monitored Plasmodium falciparum infection prevalence and gametocyte production during the dry season, and examined the impact of parasite genetic multiplicity on infection longevity. A cohort of 38 individuals who were infected with P. falciparum in November 2001 was monitored monthly by microscopy and PCR until December 2002. Reverse transcriptase polymerase chain reaction of the pfg377 gene was used to detect sub-patent gametocytes. In addition, all isolates were examined for msp-2 alleles and the mean number of parasite clones per infection was estimated. We found that a large proportion (40{\%}) of the cohort retained gametocytes throughout the dry season. The majority of patients retained asexual infection for at least 7 months. Genetic multiplicity of P. falciparum significantly influenced longevity of asexual infection and its gametocyte production. Gametocytes from mixed genotype P. falciparum infections persisted three times longer than those from single genotype infections, suggesting that genetic diversity promotes persistence. These findings are discussed in the context of the parasite biology and malaria epidemiology in the study area.",
keywords = "Gametocytes, Plasmodium falciparum, RT-PCR, Seasonal malaria",
author = "Elkhansaa Nassir and Abdel-Muhsin, {Abdel Muhsin A} and Suad Suliaman and Fiona Kenyon and Amani Kheir and Haider Geha and Ferguson, {Heather M.} and David Walliker and Babiker, {Hamza A.}",
year = "2005",
month = "1",
doi = "10.1016/j.ijpara.2004.10.014",
language = "English",
volume = "35",
pages = "49--55",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Impact of genetic complexity on longevity and gametocytogenesis of Plasmodium falciparum during the dry and transmission-free season of eastern Sudan

AU - Nassir, Elkhansaa

AU - Abdel-Muhsin, Abdel Muhsin A

AU - Suliaman, Suad

AU - Kenyon, Fiona

AU - Kheir, Amani

AU - Geha, Haider

AU - Ferguson, Heather M.

AU - Walliker, David

AU - Babiker, Hamza A.

PY - 2005/1

Y1 - 2005/1

N2 - Malaria in eastern Sudan is characterised by limited seasonal transmission, with the majority of the year remaining transmission-free. Some inhabitants who contract malaria during the transmission season retain long-lasting sub-patent infections, which probably initiate transmission the following year. Here we have monitored Plasmodium falciparum infection prevalence and gametocyte production during the dry season, and examined the impact of parasite genetic multiplicity on infection longevity. A cohort of 38 individuals who were infected with P. falciparum in November 2001 was monitored monthly by microscopy and PCR until December 2002. Reverse transcriptase polymerase chain reaction of the pfg377 gene was used to detect sub-patent gametocytes. In addition, all isolates were examined for msp-2 alleles and the mean number of parasite clones per infection was estimated. We found that a large proportion (40%) of the cohort retained gametocytes throughout the dry season. The majority of patients retained asexual infection for at least 7 months. Genetic multiplicity of P. falciparum significantly influenced longevity of asexual infection and its gametocyte production. Gametocytes from mixed genotype P. falciparum infections persisted three times longer than those from single genotype infections, suggesting that genetic diversity promotes persistence. These findings are discussed in the context of the parasite biology and malaria epidemiology in the study area.

AB - Malaria in eastern Sudan is characterised by limited seasonal transmission, with the majority of the year remaining transmission-free. Some inhabitants who contract malaria during the transmission season retain long-lasting sub-patent infections, which probably initiate transmission the following year. Here we have monitored Plasmodium falciparum infection prevalence and gametocyte production during the dry season, and examined the impact of parasite genetic multiplicity on infection longevity. A cohort of 38 individuals who were infected with P. falciparum in November 2001 was monitored monthly by microscopy and PCR until December 2002. Reverse transcriptase polymerase chain reaction of the pfg377 gene was used to detect sub-patent gametocytes. In addition, all isolates were examined for msp-2 alleles and the mean number of parasite clones per infection was estimated. We found that a large proportion (40%) of the cohort retained gametocytes throughout the dry season. The majority of patients retained asexual infection for at least 7 months. Genetic multiplicity of P. falciparum significantly influenced longevity of asexual infection and its gametocyte production. Gametocytes from mixed genotype P. falciparum infections persisted three times longer than those from single genotype infections, suggesting that genetic diversity promotes persistence. These findings are discussed in the context of the parasite biology and malaria epidemiology in the study area.

KW - Gametocytes

KW - Plasmodium falciparum

KW - RT-PCR

KW - Seasonal malaria

UR - http://www.scopus.com/inward/record.url?scp=19344377284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19344377284&partnerID=8YFLogxK

U2 - 10.1016/j.ijpara.2004.10.014

DO - 10.1016/j.ijpara.2004.10.014

M3 - Article

C2 - 15619515

AN - SCOPUS:19344377284

VL - 35

SP - 49

EP - 55

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 1

ER -