Identification of citrus polyploids using chromosome counts, morphological and SSR markers

Bilquees Fatima; Muhammad Usman; Muhammad Sarwar Khan; Iqrar Ahmad Khan; Muhammad Mumtaz Khan

Identification of citrus polyploids using chromosome counts, morphological and SSR markers

Bilquees Fatima, Muhammad Usman, Muhammad Sarwar Khan, Iqrar Ahmad Khan, Muhammad Mumtaz Khan

Crop Sciences

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

Polyploids play a key role in the improvement of citrus cultivars. Citrus breeding programs necessitate morphological, cytological and genetic characterization of cultivars. Here we analyze the polyploids using these three approaches. Cytological studies signify occurrence of spontaneous triploids (9.95%) and tetraploids (6.05%) in Kinnow mandarin and Succari sweet orange, respectively. Leaf characteristics were found directly proportional to the ploidy level; they change with an increase in the ploidy level. Genetic variability amongst morphologically distinct polyploids was confirmed using Simple Sequence Repeat (SSR) molecular markers. Accessions K ₁, K ₄, K ₅ and K ₇ of Kinnow, F ₇ , F₈ and F ₁₀ of Feutrell’s Early, M ₂ and M ₄ of Mosambi, and S ₁ and S ₁₁ of Succari were found polymorphic, and are considered useful in current breeding programs in Pakistan. SSR loci genotyping, morphological selection and cytological screening of polyploids appeared to be an improved strategy for efficient screening and characterization of polyploids at seedling stage.

Original language	English
Pages (from-to)	107-114
Number of pages	8
Journal	Pakistan Journal of Agricultural Sciences
Volume	52
Issue number	1
Publication status	Published - 2015

Keywords

Aborted seeds
Genetics
Kinnow
Phenotype
Ploidy

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science
Soil Science
Food Science

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Fatima, B., Usman, M., Khan, M. S., Khan, I. A., & Khan, M. M. (2015). Identification of citrus polyploids using chromosome counts, morphological and SSR markers. Pakistan Journal of Agricultural Sciences, 52(1), 107-114.

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abstract = "Polyploids play a key role in the improvement of citrus cultivars. Citrus breeding programs necessitate morphological, cytological and genetic characterization of cultivars. Here we analyze the polyploids using these three approaches. Cytological studies signify occurrence of spontaneous triploids (9.95%) and tetraploids (6.05%) in Kinnow mandarin and Succari sweet orange, respectively. Leaf characteristics were found directly proportional to the ploidy level; they change with an increase in the ploidy level. Genetic variability amongst morphologically distinct polyploids was confirmed using Simple Sequence Repeat (SSR) molecular markers. Accessions K 1, K 4, K 5 and K 7 of Kinnow, F 7 , F8 and F 10 of Feutrell{\textquoteright}s Early, M 2 and M 4 of Mosambi, and S 1 and S 11 of Succari were found polymorphic, and are considered useful in current breeding programs in Pakistan. SSR loci genotyping, morphological selection and cytological screening of polyploids appeared to be an improved strategy for efficient screening and characterization of polyploids at seedling stage.",

keywords = "Aborted seeds, Genetics, Kinnow, Phenotype, Ploidy",

author = "Bilquees Fatima and Muhammad Usman and Khan, {Muhammad Sarwar} and Khan, {Iqrar Ahmad} and Khan, {Muhammad Mumtaz}",

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AU - Fatima, Bilquees

AU - Usman, Muhammad

AU - Khan, Muhammad Sarwar

AU - Khan, Iqrar Ahmad

AU - Khan, Muhammad Mumtaz

PY - 2015

Y1 - 2015

N2 - Polyploids play a key role in the improvement of citrus cultivars. Citrus breeding programs necessitate morphological, cytological and genetic characterization of cultivars. Here we analyze the polyploids using these three approaches. Cytological studies signify occurrence of spontaneous triploids (9.95%) and tetraploids (6.05%) in Kinnow mandarin and Succari sweet orange, respectively. Leaf characteristics were found directly proportional to the ploidy level; they change with an increase in the ploidy level. Genetic variability amongst morphologically distinct polyploids was confirmed using Simple Sequence Repeat (SSR) molecular markers. Accessions K 1, K 4, K 5 and K 7 of Kinnow, F 7 , F8 and F 10 of Feutrell’s Early, M 2 and M 4 of Mosambi, and S 1 and S 11 of Succari were found polymorphic, and are considered useful in current breeding programs in Pakistan. SSR loci genotyping, morphological selection and cytological screening of polyploids appeared to be an improved strategy for efficient screening and characterization of polyploids at seedling stage.

AB - Polyploids play a key role in the improvement of citrus cultivars. Citrus breeding programs necessitate morphological, cytological and genetic characterization of cultivars. Here we analyze the polyploids using these three approaches. Cytological studies signify occurrence of spontaneous triploids (9.95%) and tetraploids (6.05%) in Kinnow mandarin and Succari sweet orange, respectively. Leaf characteristics were found directly proportional to the ploidy level; they change with an increase in the ploidy level. Genetic variability amongst morphologically distinct polyploids was confirmed using Simple Sequence Repeat (SSR) molecular markers. Accessions K 1, K 4, K 5 and K 7 of Kinnow, F 7 , F8 and F 10 of Feutrell’s Early, M 2 and M 4 of Mosambi, and S 1 and S 11 of Succari were found polymorphic, and are considered useful in current breeding programs in Pakistan. SSR loci genotyping, morphological selection and cytological screening of polyploids appeared to be an improved strategy for efficient screening and characterization of polyploids at seedling stage.

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