Identification of aerobic heterotrophic bacteria from the photic zone of a hypersaline microbial mat

Henk M. Jonkers, R. M M Abed

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Aerobic heterotrophic bacteria inhabiting microbial mats play an important role in carbon-cycling, yet information about their identity is scarce. We used the most probable number technique combined with denaturing gradient gel electrophoresis (DGGE) to identify apparent numerically important populations of aerobic heterotrophic bacteria in a hypersaline microbial mat. At high dilutions, populations related to the genera Rhodobacter and Roseobacter and to the genera Marinobacter and Halomonas dominated glycolate and yeast extract-amended enrichment cultures respectively. Three selected isolates of aerobic heterotrophic bacteria were obtained from glycolate-amended enrichments and found to relate phylogenetically to species of the genus Roseobacter. These strains were found to grow, in addition to glycolate, on several other photosynthetic compounds known to be produced by cyanobacteria suggesting that they specialize in mineralizing photosynthetic excretion products. Our study demonstrates that aerobic heterotrophs, which are phylogenetically affiliated to genera such as Rhodobacter, Roseobacter, Marinobacter and Halomonas, are important in hypersaline microbial mats, and that their specific in situ role in the carbon cycle needs to be further investigated.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalAquatic Microbial Ecology
Volume30
Issue number2
Publication statusPublished - Jan 7 2003

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glycolic acid
Roseobacter
Aerobic Bacteria
photic zone
microbial mat
Marinobacter
Rhodobacter
euphotic zone
Halomonas
bacterium
bacteria
Carbon Cycle
Denaturing Gradient Gel Electrophoresis
enrichment culture
heterotrophs
Cyanobacteria
denaturing gradient gel electrophoresis
yeast extract
carbon cycle
excretion

Keywords

  • DGGE
  • Microbial mat
  • Photosynthate-using bacteria
  • Phylogeny

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Identification of aerobic heterotrophic bacteria from the photic zone of a hypersaline microbial mat. / Jonkers, Henk M.; Abed, R. M M.

In: Aquatic Microbial Ecology, Vol. 30, No. 2, 07.01.2003, p. 127-133.

Research output: Contribution to journalArticle

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