Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman

Adel A. Al Shihi, Abdullah M. Al Sadi, Mike Deadman, Rob W. Briddon, Muhammad S. Shahid

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tomato (Solanum lycopersicum) plants exhibiting yellowing, curling and stunting symptoms were identified in fields of the Tawoos Agricultural Systems, in Al-Batinah in Oman. Cloning and sequencing of restriction endonuclease digested rolling circle amplified viral DNA identified a cotton begomovirus (family Geminiviridae) associated with the symptomatic tomato plants. Detailed analysis of complete sequences showed the virus to be a previously unknown strain of Cotton leaf curl Gezira virus (CLCuGeV) in association with the betasatellite Tomato leaf curl betasatellite (ToLCB). The new CLCuGeV strain, for which the name “Al Batinah” strain is suggested, has the greatest levels of sequence identity (91.9%) to an isolate of CLCuGeV recently reported from the neighbouring United Arab Emirates. Additionally, CLCuGeV-Al Batinah was shown to have a recombinant origin with sequences donated by an African cassava mosaic virus-like parent. This is the first identification of this Malvaceae-adapted begomovirus in tomato. Although ToLCB is common in Oman, being one of only two betasatellites identified there so far, this is the first identification of this betasatellite with CLCuGeV. The significance of these findings is discussed.

Original languageEnglish
Pages (from-to)199-205
Number of pages7
JournalJournal of Phytopathology
Volume166
Issue number3
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Oman
Lycopersicon esculentum
tomatoes
Viruses
Begomovirus
leaf curling
African cassava mosaic virus
Malvaceae
Geminiviridae
United Arab Emirates
Growth Disorders
restriction endonucleases
Solanum lycopersicum
Mosaic Viruses
Manihot
DNA Restriction Enzymes
growth retardation
Viral DNA
signs and symptoms (plants)
molecular cloning

Keywords

  • begomovirus
  • betasatellite
  • Cotton leaf curl Gezira virus
  • Solanum lycopersicum

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Identification of a distinct strain of Cotton leaf curl Gezira virus infecting tomato in Oman. / Al Shihi, Adel A.; Al Sadi, Abdullah M.; Deadman, Mike; Briddon, Rob W.; Shahid, Muhammad S.

In: Journal of Phytopathology, Vol. 166, No. 3, 01.03.2018, p. 199-205.

Research output: Contribution to journalArticle

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