Human gastric mucosal hydrophobicity does not decrease with Helicobacter pylori infection or chronological age

Mohammed S. Al-Marhoon, Sheila Nunn, Roger W. Soames

Research output: Contribution to journalArticle

Abstract

Background and aims: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases. Previous studies in humans have reported a decreased gastric hydrophobicity with H pylori infection. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus hydrophobicity. Methods: One hundred patients without peptic ulcers and not on medication were randomly recruited from endoscopy clinics; each patient had six biopsies. Contact angle measurements were performed using a goniometer assisted by computer software. H pylori status was assessed by histology, Campylobacter-like organism test and culture, and cagA+ status was determined by polymerase chain reaction. Results: In age- and sex-matched patients, there was no significant difference (P=0.27) in contact angle between H pylori-positive (61±2.8°) and H pylori-negative patients (65.5±3.0°). There was also no significant difference (P=0.36) in contact angle among H pylori-negative, cagA- and cagA+ patients (65.5±3.0°, 58.6±3.6° and 63.4±4.9°, respectively). However, a trend of increased mean contact angles in cagA+ compared with cagA- and H pylori-negative patients was observed in patients 50 years and younger (68.3±8.3°, 61.1±6.1° and 63.6±2.2°, respectively; P=0.70) and in patients without atrophy (71.1±8°, 59.6±4° and 66±2°, respectively; P=0.30). In addition, there was no significant correlation between contact angles and patient age (r=0.104, P=0.306). Conclusions: The present study shows that H pylori infection and the chronological age have no effect on the gastric mucus hydrophobicity, but it highlights a trend of increased mucus hydrophobicity with cagA+ infection that needs to be supported by future studies.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalCanadian Journal of Gastroenterology
Volume19
Issue number1
Publication statusPublished - Jan 2005

Fingerprint

Cytotoxins
Helicobacter Infections
Hydrophobic and Hydrophilic Interactions
Helicobacter pylori
Pylorus
Stomach
Genes
Mucus
Infection
Stomach Diseases
Campylobacter
Peptic Ulcer
Endoscopy
Atrophy
Histology
Software
Biopsy
Polymerase Chain Reaction

Keywords

  • Age
  • CagA+
  • Gastric
  • Helicobacter pylori
  • Hydrophobicity

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Human gastric mucosal hydrophobicity does not decrease with Helicobacter pylori infection or chronological age. / Al-Marhoon, Mohammed S.; Nunn, Sheila; Soames, Roger W.

In: Canadian Journal of Gastroenterology, Vol. 19, No. 1, 01.2005, p. 37-41.

Research output: Contribution to journalArticle

@article{b70156ed25f24c7c867dded5d5fb517c,
title = "Human gastric mucosal hydrophobicity does not decrease with Helicobacter pylori infection or chronological age",
abstract = "Background and aims: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases. Previous studies in humans have reported a decreased gastric hydrophobicity with H pylori infection. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus hydrophobicity. Methods: One hundred patients without peptic ulcers and not on medication were randomly recruited from endoscopy clinics; each patient had six biopsies. Contact angle measurements were performed using a goniometer assisted by computer software. H pylori status was assessed by histology, Campylobacter-like organism test and culture, and cagA+ status was determined by polymerase chain reaction. Results: In age- and sex-matched patients, there was no significant difference (P=0.27) in contact angle between H pylori-positive (61±2.8°) and H pylori-negative patients (65.5±3.0°). There was also no significant difference (P=0.36) in contact angle among H pylori-negative, cagA- and cagA+ patients (65.5±3.0°, 58.6±3.6° and 63.4±4.9°, respectively). However, a trend of increased mean contact angles in cagA+ compared with cagA- and H pylori-negative patients was observed in patients 50 years and younger (68.3±8.3°, 61.1±6.1° and 63.6±2.2°, respectively; P=0.70) and in patients without atrophy (71.1±8°, 59.6±4° and 66±2°, respectively; P=0.30). In addition, there was no significant correlation between contact angles and patient age (r=0.104, P=0.306). Conclusions: The present study shows that H pylori infection and the chronological age have no effect on the gastric mucus hydrophobicity, but it highlights a trend of increased mucus hydrophobicity with cagA+ infection that needs to be supported by future studies.",
keywords = "Age, CagA+, Gastric, Helicobacter pylori, Hydrophobicity",
author = "Al-Marhoon, {Mohammed S.} and Sheila Nunn and Soames, {Roger W.}",
year = "2005",
month = "1",
language = "English",
volume = "19",
pages = "37--41",
journal = "Canadian Journal of Gastroenterology and Hepatology",
issn = "2291-2789",
publisher = "Pulsus Group Inc.",
number = "1",

}

TY - JOUR

T1 - Human gastric mucosal hydrophobicity does not decrease with Helicobacter pylori infection or chronological age

AU - Al-Marhoon, Mohammed S.

AU - Nunn, Sheila

AU - Soames, Roger W.

PY - 2005/1

Y1 - 2005/1

N2 - Background and aims: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases. Previous studies in humans have reported a decreased gastric hydrophobicity with H pylori infection. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus hydrophobicity. Methods: One hundred patients without peptic ulcers and not on medication were randomly recruited from endoscopy clinics; each patient had six biopsies. Contact angle measurements were performed using a goniometer assisted by computer software. H pylori status was assessed by histology, Campylobacter-like organism test and culture, and cagA+ status was determined by polymerase chain reaction. Results: In age- and sex-matched patients, there was no significant difference (P=0.27) in contact angle between H pylori-positive (61±2.8°) and H pylori-negative patients (65.5±3.0°). There was also no significant difference (P=0.36) in contact angle among H pylori-negative, cagA- and cagA+ patients (65.5±3.0°, 58.6±3.6° and 63.4±4.9°, respectively). However, a trend of increased mean contact angles in cagA+ compared with cagA- and H pylori-negative patients was observed in patients 50 years and younger (68.3±8.3°, 61.1±6.1° and 63.6±2.2°, respectively; P=0.70) and in patients without atrophy (71.1±8°, 59.6±4° and 66±2°, respectively; P=0.30). In addition, there was no significant correlation between contact angles and patient age (r=0.104, P=0.306). Conclusions: The present study shows that H pylori infection and the chronological age have no effect on the gastric mucus hydrophobicity, but it highlights a trend of increased mucus hydrophobicity with cagA+ infection that needs to be supported by future studies.

AB - Background and aims: Infection with cytotoxin-associated gene A (cagA) Helicobacter pylori is associated with severe gastric diseases. Previous studies in humans have reported a decreased gastric hydrophobicity with H pylori infection. The aim of the present study was to differentiate between the effect of cagA+ and cagA- strains on gastric mucus hydrophobicity. Methods: One hundred patients without peptic ulcers and not on medication were randomly recruited from endoscopy clinics; each patient had six biopsies. Contact angle measurements were performed using a goniometer assisted by computer software. H pylori status was assessed by histology, Campylobacter-like organism test and culture, and cagA+ status was determined by polymerase chain reaction. Results: In age- and sex-matched patients, there was no significant difference (P=0.27) in contact angle between H pylori-positive (61±2.8°) and H pylori-negative patients (65.5±3.0°). There was also no significant difference (P=0.36) in contact angle among H pylori-negative, cagA- and cagA+ patients (65.5±3.0°, 58.6±3.6° and 63.4±4.9°, respectively). However, a trend of increased mean contact angles in cagA+ compared with cagA- and H pylori-negative patients was observed in patients 50 years and younger (68.3±8.3°, 61.1±6.1° and 63.6±2.2°, respectively; P=0.70) and in patients without atrophy (71.1±8°, 59.6±4° and 66±2°, respectively; P=0.30). In addition, there was no significant correlation between contact angles and patient age (r=0.104, P=0.306). Conclusions: The present study shows that H pylori infection and the chronological age have no effect on the gastric mucus hydrophobicity, but it highlights a trend of increased mucus hydrophobicity with cagA+ infection that needs to be supported by future studies.

KW - Age

KW - CagA+

KW - Gastric

KW - Helicobacter pylori

KW - Hydrophobicity

UR - http://www.scopus.com/inward/record.url?scp=14544281602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14544281602&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 37

EP - 41

JO - Canadian Journal of Gastroenterology and Hepatology

JF - Canadian Journal of Gastroenterology and Hepatology

SN - 2291-2789

IS - 1

ER -