HRMAS-NMR spectroscopy and multivariate analysis meat characterisation

Mena Ritota, Lorena Casciani, Sebastiana Failla, Massimiliano Valentini

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

1H-high resolution magic angle spinning-nuclear magnetic resonance spectroscopy was employed to gain the metabolic profile of longissimus dorsi and semitendinosus muscles of four different breeds: Chianina, Holstein Friesian, Maremmana and Buffalo.Principal component analysis, partial least squares projection to latent structure - discriminant analysis and orthogonal partial least squares projection to latent structure - discriminant analysis were used to build models capable of discriminating the muscle type according to the breed. Data analysis led to an excellent classification for Buffalo and Chianina, while for Holstein Friesian the separation was lower. In the case of Maremmana the use of intelligent bucketing was necessary due to some resonances shifting allowed improvement of the discrimination ability. Finally, by using the Variable Importance in Projection values the metabolites relevant for the classification were identified.

Original languageEnglish
Pages (from-to)754-761
Number of pages8
JournalMeat Science
Volume92
Issue number4
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Chianina
Buffaloes
Discriminant Analysis
Least-Squares Analysis
discriminant analysis
Meat
multivariate analysis
buffaloes
least squares
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Holstein
Multivariate Analysis
meat
breeds
semitendinosus muscle
Muscles
Metabolome
spinning
Principal Component Analysis

Keywords

  • Intelligent bucketing
  • Longissimus dorsi
  • NMR assignment
  • PLS-DA
  • Semitendinosus

ASJC Scopus subject areas

  • Food Science

Cite this

HRMAS-NMR spectroscopy and multivariate analysis meat characterisation. / Ritota, Mena; Casciani, Lorena; Failla, Sebastiana; Valentini, Massimiliano.

In: Meat Science, Vol. 92, No. 4, 12.2012, p. 754-761.

Research output: Contribution to journalArticle

Ritota, Mena ; Casciani, Lorena ; Failla, Sebastiana ; Valentini, Massimiliano. / HRMAS-NMR spectroscopy and multivariate analysis meat characterisation. In: Meat Science. 2012 ; Vol. 92, No. 4. pp. 754-761.
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