Host-defense and trefoil factor family peptides in skin secretions of the Mawa clawed frog Xenopus boumbaensis (Pipidae)

J. Michael Conlon, Milena Mechkarska, Jolanta Kolodziejek, Jérôme Leprince, Laurent Coquet, Thierry Jouenne, Hubert Vaudry, Norbert Nowotny, Jay D. King

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Peptidomic analysis of norepinephrine-stimulated skin secretions from the octoploid Mawa clawed frog Xenopus boumbaensis Loumont, 1983 led to the identification and characterization of 15 host-defense peptides belonging to the magainin (two peptides), peptide glycine-leucine-amide (PGLa; three peptides), xenopsin precursor fragment (XPF; three peptides), caerulein precursor fragment (CPF; two peptides), and caerulein precursor fragment-related peptide (CPF-RP; five peptides) families. In addition, caerulein and three peptides with structural similarity to the trefoil factor family (TFF) peptides, xP2 and xP4 from Xenopus laevis were also present in the secretions. Consistent with data from comparisons of the nucleotides sequence of mitochondrial and nuclear genes, the primary structures of the peptides suggest a close phylogenetic relationship between X. boumbaensis and the octoploid frogs Xenopus amieti and Xenopus andrei. As the three species occupy disjunct ranges within Cameroon, it is suggested that they diverged from a common ancestor by allopatric speciation.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalPeptides
Volume72
DOIs
Publication statusPublished - Feb 28 2015

Keywords

  • Frog skin
  • Host-defense
  • Pipidae
  • Polyploidy
  • Trefoil factor family
  • Xenopus

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Conlon, J. M., Mechkarska, M., Kolodziejek, J., Leprince, J., Coquet, L., Jouenne, T., Vaudry, H., Nowotny, N., & King, J. D. (2015). Host-defense and trefoil factor family peptides in skin secretions of the Mawa clawed frog Xenopus boumbaensis (Pipidae). Peptides, 72, 44-49. https://doi.org/10.1016/j.peptides.2015.03.025