High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates

Ágnes Sonnevend, Vincent O. Rotimi, Jolanta Kolodziejek, Asad Usmani, Norbert Nowotny, Tibor Pál

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The antibiotic sensitivity and the serotype and molecular type (MT) distribution of 41 Campylobacter jejuni strains isolated from individual patients in Tawam Hospital, Al Ain, United Arab Emirates, were investigated. While all strains were sensitive to erythromycin (MIC 0.5-4 mg l-1), 35 isolates (85.4%) exhibited resistance to ciprofloxacin (MIC 8-64 mg l -1). All resistant strains carried the Thr-86 to He mutation in the gyrase A (gyrA) gene, as shown by mismatch amplification mutation assay (MAMA) and confirmed by sequencing. Based on the partial sequences of gyrA, resistant isolates carried 10 distinct alleles, eight of them representing new variants. Strains were assigned to 30 MTs based on the combined results of PFGE and flaA PCR-RFLP typing. Eight of the 35 ciprofloxacin-resistant strains, isolated over a period of more than 1 year, represented the largest MT, also carrying the same allelic variant of the gyrA gene. These results show that the local incidence of fluoroquinolone resistance among C. jejuni is one of the highest reported worldwide. It was also demonstrated that stable MTs could persist for a relatively long time among the clonally unrelated antibiotic-resistant isolates of C. jejuni. The data also emphasize the need to replace fluoroquinolones as empirical therapy for diarrhoea of undiagnosed aetiology.

Original languageEnglish
Pages (from-to)1533-1538
Number of pages6
JournalJournal of Medical Microbiology
Volume55
Issue number11
DOIs
Publication statusPublished - Nov 2006

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United Arab Emirates
Campylobacter jejuni
Ciprofloxacin
Fluoroquinolones
Anti-Bacterial Agents
Mutation
Erythromycin
Restriction Fragment Length Polymorphisms
Genes
Diarrhea
Alleles
Polymerase Chain Reaction
Incidence
Therapeutics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates. / Sonnevend, Ágnes; Rotimi, Vincent O.; Kolodziejek, Jolanta; Usmani, Asad; Nowotny, Norbert; Pál, Tibor.

In: Journal of Medical Microbiology, Vol. 55, No. 11, 11.2006, p. 1533-1538.

Research output: Contribution to journalArticle

Sonnevend, Ágnes ; Rotimi, Vincent O. ; Kolodziejek, Jolanta ; Usmani, Asad ; Nowotny, Norbert ; Pál, Tibor. / High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates. In: Journal of Medical Microbiology. 2006 ; Vol. 55, No. 11. pp. 1533-1538.
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