Growth restricted in vitro culture conditions alter the imprinted gene expression patterns of mouse embryonic stem cells

Senan Baqir, Lawrence C. Smith

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Embryonic stem (ES) cell-derived clones and chimeras are often associated with growth abnormalities during fetal development, leading to the production of over/under-weight offspring that show elevated neonatal mortality and morbidity. Due to the role played by imprinted genes in controlling fetal growth, much of the blame is pointed at improper epigenetic reprogramming of cells used in the procedures. We have analyzed the expression pattern of two growth regulatory imprinted genes, namely insulin like growth factor II (Igf2) and H19, in mouse ES cells cultured under growth restricted conditions and after in vitro aging. Culture of cells with serum-depleted media (starvation) and at high cell density (confluence) increased the expression of both imprinted genes and led to aberrant methylation profiles of differentially methylated regions in key regulatory sites of Igf2 and H19. These findings confirm that growth constrained cultures of ES cells are associated with alterations to methylation of the regulatory domains and the expression patterns of imprinted genes, suggesting a possible role of epigenetic factors in the loss of developmental potential.

Original languageEnglish
Pages (from-to)199-212
Number of pages14
JournalCloning and Stem Cells
Volume5
Issue number3
Publication statusPublished - 2003

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Gene Expression
Embryonic Stem Cells
Growth
Fetal Development
Epigenomics
Methylation
Genes
Insulin-Like Growth Factor II
Infant Mortality
Regulator Genes
Starvation
Clone Cells
Cell Culture Techniques
Cell Count
Morbidity
Weights and Measures
In Vitro Techniques
Mouse Embryonic Stem Cells
Serum
Cellular Reprogramming

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Applied Microbiology and Biotechnology

Cite this

Growth restricted in vitro culture conditions alter the imprinted gene expression patterns of mouse embryonic stem cells. / Baqir, Senan; Smith, Lawrence C.

In: Cloning and Stem Cells, Vol. 5, No. 3, 2003, p. 199-212.

Research output: Contribution to journalArticle

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