Graphene-amplified femtosensitive aptasensing of estradiol, an endocrine disruptor

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report the construction of a novel electrochemical femtomolar aptasensing APT-ERGO/GCE interface based on the covalent immobilization of 38-mer amine-functionalized (NH2-APT) 17β-estradiol (E2) DNA aptamers on a graphene amplifying platform. Graphene oxide (GO) was synthesized and characterized by using FTIR, UV-vis spectroscopy, XRD spectroscopy, and SEM technique. The strategy for the construction of the E2-aptasensing interface involves in a three-step modification process. (i) First, we carried out the electrochemical reduction of GO on the GCE electrode to form ERGO/GCE. (ii) Then, as an impact strategy, the E2-aptamers (NH2-APT) were further immobilized on the surface of the ERGO/GCE interface through electrochemical reduction of surface-functionalized diazonium salts. This step includes electrografting of ERGO/GCE by electrochemical reduction of the diazonium salt (ClN2 +-Ph-COOH) to obtain the ERGO/GCE-Ph-COOH-modified electrode. (iii) Finally, the free carboxyl groups on the ERGO/GCE-Ph-COOH surface were conjugated with NH2-APT through formation of carbodiimide to afford an aptasensing APT-ERGO/GCE interface. The presence of ERGO as an amplifying platform led to the successful immobilization of E2-aptamers with a surface coverage of 1.9 × 1013 molecule per cm2, which is higher than the values obtained in other reported methods. The constructed aptasensing APT-ERGO/GCE interface was appraised using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The synergetic effect of high affinity and specificity of E2-aptamers and graphene platform was beneficial for the novel femtosensitive label-free electrochemical aptasensing APT-ERGO/GCE interface for the detection of [E2]. The oxidation current peaks at the aptasensing APT-ERGO/GCE interface were proportional to [E2] over two different concentration linearity ranges 1.0 × 10-15 mol L-1-9.0 × 10-12 mol L-1-1.2 × 10-11 mol L-1 to 2.3 × 10-10 mol L-1 with a limit of detection (LOD) of 0.5 × 10-15 mol L-1. This aptasensing APT-ERGO/GCE interface was employed as a femtomolar tool for the determination of [E2] in the environmental and pharmaceutical samples such as wastewater (spiked) and pharmaceutical dosages.

Original languageEnglish
Pages (from-to)1835-1845
Number of pages11
JournalAnalyst
Volume143
Issue number8
DOIs
Publication statusPublished - Apr 21 2018

Fingerprint

Endocrine Disruptors
endocrine disruptor
Graphite
Graphene
Estradiol
Drug products
Salts
Electrodes
Oxides
spectroscopy
Ultraviolet spectroscopy
Electrochemical impedance spectroscopy
immobilization
Cyclic voltammetry
Labels
Amines
Immobilization
electrode
Wastewater
drug

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Graphene-amplified femtosensitive aptasensing of estradiol, an endocrine disruptor. / Rather, Jahangir Ahmad; Khudaish, Emad A.; Kannan, Palanisamy.

In: Analyst, Vol. 143, No. 8, 21.04.2018, p. 1835-1845.

Research output: Contribution to journalArticle

@article{9253dc83fd1e4865a26fe33980e94534,
title = "Graphene-amplified femtosensitive aptasensing of estradiol, an endocrine disruptor",
abstract = "We report the construction of a novel electrochemical femtomolar aptasensing APT-ERGO/GCE interface based on the covalent immobilization of 38-mer amine-functionalized (NH2-APT) 17β-estradiol (E2) DNA aptamers on a graphene amplifying platform. Graphene oxide (GO) was synthesized and characterized by using FTIR, UV-vis spectroscopy, XRD spectroscopy, and SEM technique. The strategy for the construction of the E2-aptasensing interface involves in a three-step modification process. (i) First, we carried out the electrochemical reduction of GO on the GCE electrode to form ERGO/GCE. (ii) Then, as an impact strategy, the E2-aptamers (NH2-APT) were further immobilized on the surface of the ERGO/GCE interface through electrochemical reduction of surface-functionalized diazonium salts. This step includes electrografting of ERGO/GCE by electrochemical reduction of the diazonium salt (ClN2 +-Ph-COOH) to obtain the ERGO/GCE-Ph-COOH-modified electrode. (iii) Finally, the free carboxyl groups on the ERGO/GCE-Ph-COOH surface were conjugated with NH2-APT through formation of carbodiimide to afford an aptasensing APT-ERGO/GCE interface. The presence of ERGO as an amplifying platform led to the successful immobilization of E2-aptamers with a surface coverage of 1.9 × 1013 molecule per cm2, which is higher than the values obtained in other reported methods. The constructed aptasensing APT-ERGO/GCE interface was appraised using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The synergetic effect of high affinity and specificity of E2-aptamers and graphene platform was beneficial for the novel femtosensitive label-free electrochemical aptasensing APT-ERGO/GCE interface for the detection of [E2]. The oxidation current peaks at the aptasensing APT-ERGO/GCE interface were proportional to [E2] over two different concentration linearity ranges 1.0 × 10-15 mol L-1-9.0 × 10-12 mol L-1-1.2 × 10-11 mol L-1 to 2.3 × 10-10 mol L-1 with a limit of detection (LOD) of 0.5 × 10-15 mol L-1. This aptasensing APT-ERGO/GCE interface was employed as a femtomolar tool for the determination of [E2] in the environmental and pharmaceutical samples such as wastewater (spiked) and pharmaceutical dosages.",
author = "Rather, {Jahangir Ahmad} and Khudaish, {Emad A.} and Palanisamy Kannan",
year = "2018",
month = "4",
day = "21",
doi = "10.1039/c7an02092a",
language = "English",
volume = "143",
pages = "1835--1845",
journal = "The Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "8",

}

TY - JOUR

T1 - Graphene-amplified femtosensitive aptasensing of estradiol, an endocrine disruptor

AU - Rather, Jahangir Ahmad

AU - Khudaish, Emad A.

AU - Kannan, Palanisamy

PY - 2018/4/21

Y1 - 2018/4/21

N2 - We report the construction of a novel electrochemical femtomolar aptasensing APT-ERGO/GCE interface based on the covalent immobilization of 38-mer amine-functionalized (NH2-APT) 17β-estradiol (E2) DNA aptamers on a graphene amplifying platform. Graphene oxide (GO) was synthesized and characterized by using FTIR, UV-vis spectroscopy, XRD spectroscopy, and SEM technique. The strategy for the construction of the E2-aptasensing interface involves in a three-step modification process. (i) First, we carried out the electrochemical reduction of GO on the GCE electrode to form ERGO/GCE. (ii) Then, as an impact strategy, the E2-aptamers (NH2-APT) were further immobilized on the surface of the ERGO/GCE interface through electrochemical reduction of surface-functionalized diazonium salts. This step includes electrografting of ERGO/GCE by electrochemical reduction of the diazonium salt (ClN2 +-Ph-COOH) to obtain the ERGO/GCE-Ph-COOH-modified electrode. (iii) Finally, the free carboxyl groups on the ERGO/GCE-Ph-COOH surface were conjugated with NH2-APT through formation of carbodiimide to afford an aptasensing APT-ERGO/GCE interface. The presence of ERGO as an amplifying platform led to the successful immobilization of E2-aptamers with a surface coverage of 1.9 × 1013 molecule per cm2, which is higher than the values obtained in other reported methods. The constructed aptasensing APT-ERGO/GCE interface was appraised using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The synergetic effect of high affinity and specificity of E2-aptamers and graphene platform was beneficial for the novel femtosensitive label-free electrochemical aptasensing APT-ERGO/GCE interface for the detection of [E2]. The oxidation current peaks at the aptasensing APT-ERGO/GCE interface were proportional to [E2] over two different concentration linearity ranges 1.0 × 10-15 mol L-1-9.0 × 10-12 mol L-1-1.2 × 10-11 mol L-1 to 2.3 × 10-10 mol L-1 with a limit of detection (LOD) of 0.5 × 10-15 mol L-1. This aptasensing APT-ERGO/GCE interface was employed as a femtomolar tool for the determination of [E2] in the environmental and pharmaceutical samples such as wastewater (spiked) and pharmaceutical dosages.

AB - We report the construction of a novel electrochemical femtomolar aptasensing APT-ERGO/GCE interface based on the covalent immobilization of 38-mer amine-functionalized (NH2-APT) 17β-estradiol (E2) DNA aptamers on a graphene amplifying platform. Graphene oxide (GO) was synthesized and characterized by using FTIR, UV-vis spectroscopy, XRD spectroscopy, and SEM technique. The strategy for the construction of the E2-aptasensing interface involves in a three-step modification process. (i) First, we carried out the electrochemical reduction of GO on the GCE electrode to form ERGO/GCE. (ii) Then, as an impact strategy, the E2-aptamers (NH2-APT) were further immobilized on the surface of the ERGO/GCE interface through electrochemical reduction of surface-functionalized diazonium salts. This step includes electrografting of ERGO/GCE by electrochemical reduction of the diazonium salt (ClN2 +-Ph-COOH) to obtain the ERGO/GCE-Ph-COOH-modified electrode. (iii) Finally, the free carboxyl groups on the ERGO/GCE-Ph-COOH surface were conjugated with NH2-APT through formation of carbodiimide to afford an aptasensing APT-ERGO/GCE interface. The presence of ERGO as an amplifying platform led to the successful immobilization of E2-aptamers with a surface coverage of 1.9 × 1013 molecule per cm2, which is higher than the values obtained in other reported methods. The constructed aptasensing APT-ERGO/GCE interface was appraised using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The synergetic effect of high affinity and specificity of E2-aptamers and graphene platform was beneficial for the novel femtosensitive label-free electrochemical aptasensing APT-ERGO/GCE interface for the detection of [E2]. The oxidation current peaks at the aptasensing APT-ERGO/GCE interface were proportional to [E2] over two different concentration linearity ranges 1.0 × 10-15 mol L-1-9.0 × 10-12 mol L-1-1.2 × 10-11 mol L-1 to 2.3 × 10-10 mol L-1 with a limit of detection (LOD) of 0.5 × 10-15 mol L-1. This aptasensing APT-ERGO/GCE interface was employed as a femtomolar tool for the determination of [E2] in the environmental and pharmaceutical samples such as wastewater (spiked) and pharmaceutical dosages.

UR - http://www.scopus.com/inward/record.url?scp=85045531543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045531543&partnerID=8YFLogxK

U2 - 10.1039/c7an02092a

DO - 10.1039/c7an02092a

M3 - Article

AN - SCOPUS:85045531543

VL - 143

SP - 1835

EP - 1845

JO - The Analyst

JF - The Analyst

SN - 0003-2654

IS - 8

ER -