Gentamicin nephrotoxicity in rat: Some biochemical correlates

B. H. Ali, A. A. Abdel Gayoum, A. A. Bashir

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The present work examines the effect of treatment of rats with graded doses of the aminoglycoside antibiotic gentamicin on the concentration of reduced glutathione (GSH) and diamine oxidase (DAO) activity in the kidney, and DAO activity, creatinine and magnesium (Mg) in the plasma. The animals were given the antibiotic intramuscularly in doses of 20, 40, and 80 mg/kg/day for 6 days, and were killed 24 h after the last injection. In another experiment rats were injected intramuscularly with gentamicin at a dose of 80 mg/kg/day for 6 days and were killed 1, 7 or 14 days after the last injection, and the above parameters were measured. Gentamicin reduced the body weights of rats in a dose-dependent manner. The weight reductions were most marked on days 4, 5 and 6 of the treatment. The body weights gradually recovered on withdrawing of the drug, and by day 14, they were not significantly different from those of the controls. Gentamicin produced significant and dose-dependent decreases in the renal concentration of GSH. Seven and 14 days after withdrawing the drug, the GSH concentrations were still significantly below that of the controls. Plasma Mg concentrations were significantly decreased, and plasma creatinine concentrations significantly increased by gentamicin. These effects persisted 7 and 14 days after cessation of treatment. Plasma DAO activity was not detectable in the control or gentamicin-treated rats. In the renal cortex, the activity of the enzyme, measured 1, 7 and 14 days after the treatment, was not significantly different from that of the control. Histopathologically, the drug produced dose-dependent proximal renal tubular necrosis. Seven days after withdrawal of gentamicin, the degree of necrosis was less marked, and 14 days after drug withdrawal, renal histology was apparently normal.

Original languageEnglish
Pages (from-to)419-423
Number of pages5
JournalPharmacology and Toxicology
Volume70
Issue number6 I
Publication statusPublished - 1992

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Gentamicins
Rats
Amine Oxidase (Copper-Containing)
Kidney
Plasmas
Pharmaceutical Preparations
Magnesium
Creatinine
Necrosis
Body Weight
Anti-Bacterial Agents
Injections
Withholding Treatment
Histology
Aminoglycosides
Glutathione
Weight Loss
Animals
Therapeutics
Enzymes

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pharmacology
  • Toxicology

Cite this

Ali, B. H., Abdel Gayoum, A. A., & Bashir, A. A. (1992). Gentamicin nephrotoxicity in rat: Some biochemical correlates. Pharmacology and Toxicology, 70(6 I), 419-423.

Gentamicin nephrotoxicity in rat : Some biochemical correlates. / Ali, B. H.; Abdel Gayoum, A. A.; Bashir, A. A.

In: Pharmacology and Toxicology, Vol. 70, No. 6 I, 1992, p. 419-423.

Research output: Contribution to journalArticle

Ali, BH, Abdel Gayoum, AA & Bashir, AA 1992, 'Gentamicin nephrotoxicity in rat: Some biochemical correlates', Pharmacology and Toxicology, vol. 70, no. 6 I, pp. 419-423.
Ali, B. H. ; Abdel Gayoum, A. A. ; Bashir, A. A. / Gentamicin nephrotoxicity in rat : Some biochemical correlates. In: Pharmacology and Toxicology. 1992 ; Vol. 70, No. 6 I. pp. 419-423.
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