Gene expression profiling of host response in models of acute HIV infection

Steven E. Bosinger, Karoline A. Hosiawa, Mark J. Cameron, Desmond Persad, Longsi Ran, Luoling Xu, Mohamed R. Boulassel, Monique Parenteau, Jocelyn Fournier, Erling W. Rud, David J. Kelvin

Research output: Contribution to journalArticle

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Abstract

HIV infection is characterized by a host response composed of adaptive and innate immunity that partially limits viral replication; however, it ultimately fails in eradicating the virus. To model host gene expression during acute HIV infection, we infected cynomolgus macaques with the SIV/HIV-1 chimeric virus, SHIV89.6P, and profiled gene expression in peripheral blood over a 5-wk period using a high density cDNA microarray. We demonstrate that viral challenge induced a widespread suppression of genes regulating innate immunity, including the LPS receptors, CD14 and TLR4. An overexpression of 16 IFN-stimulated genes was also observed in response to infection; however, it did not correlate with control over viral titers. A statistical analysis of the dataset identified 10 genes regulating apoptosis with differential expression during the first 2 wk of infection (p < 0.004). Quantitative real-time PCR verified transcriptional increases in IFN-α-inducible genes and decreases in genes regulating innate immunity. Therefore, the persistence of high viral loads despite an extensive IFN response suggests that HIV can resist in vivo IFN treatment despite published reports of in vitro efficacy. The transcriptional suppression of genes regulating innate immunity may allow HIV to evade acute host responses and establish a chronic infection and may reduce innate host defense against opportunistic infections.

Original languageEnglish
Pages (from-to)6858-6863
Number of pages6
JournalJournal of Immunology
Volume173
Issue number11
Publication statusPublished - Dec 1 2004

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Gene Expression Profiling
HIV Infections
Innate Immunity
Genes
Infection
HIV
CD14 Antigens
Viruses
Gene Expression
Opportunistic Infections
Macaca
Adaptive Immunity
Oligonucleotide Array Sequence Analysis
Viral Load
HIV-1
Real-Time Polymerase Chain Reaction
Apoptosis

ASJC Scopus subject areas

  • Immunology

Cite this

Bosinger, S. E., Hosiawa, K. A., Cameron, M. J., Persad, D., Ran, L., Xu, L., ... Kelvin, D. J. (2004). Gene expression profiling of host response in models of acute HIV infection. Journal of Immunology, 173(11), 6858-6863.

Gene expression profiling of host response in models of acute HIV infection. / Bosinger, Steven E.; Hosiawa, Karoline A.; Cameron, Mark J.; Persad, Desmond; Ran, Longsi; Xu, Luoling; Boulassel, Mohamed R.; Parenteau, Monique; Fournier, Jocelyn; Rud, Erling W.; Kelvin, David J.

In: Journal of Immunology, Vol. 173, No. 11, 01.12.2004, p. 6858-6863.

Research output: Contribution to journalArticle

Bosinger, SE, Hosiawa, KA, Cameron, MJ, Persad, D, Ran, L, Xu, L, Boulassel, MR, Parenteau, M, Fournier, J, Rud, EW & Kelvin, DJ 2004, 'Gene expression profiling of host response in models of acute HIV infection', Journal of Immunology, vol. 173, no. 11, pp. 6858-6863.
Bosinger SE, Hosiawa KA, Cameron MJ, Persad D, Ran L, Xu L et al. Gene expression profiling of host response in models of acute HIV infection. Journal of Immunology. 2004 Dec 1;173(11):6858-6863.
Bosinger, Steven E. ; Hosiawa, Karoline A. ; Cameron, Mark J. ; Persad, Desmond ; Ran, Longsi ; Xu, Luoling ; Boulassel, Mohamed R. ; Parenteau, Monique ; Fournier, Jocelyn ; Rud, Erling W. ; Kelvin, David J. / Gene expression profiling of host response in models of acute HIV infection. In: Journal of Immunology. 2004 ; Vol. 173, No. 11. pp. 6858-6863.
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