Frequency of TLR4 (1063A/G and 1363C/T) polymorphisms in healthy and HIV-infected Omani individuals and their relationship to viral load and T cell count

E. A. Said, F. Al-Yafei, F. Zadjali, M. S. Al-Balushi, S. S. Hasson, S. H. Al-Mahroqi, C. Y. Koh, K. Al-Naamani, J. Z. Al-Busaidi, M. A. Idris, A. Balkhair, A. A. Al-Jabri

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Abstract

Toll-like receptors (TLRs) are essential elements of the innate immune response to different infections including the infection with human immunodeficiency virus (HIV). Single nucleotide polymorphisms (SNPs) in TLRs such as TLR4 1063A/G and 1363C/T have been found to be associated with changes in CD4 count, viral load (VL), and disease progression during HIV infection. However, the association of these SNPs with the pathogenesis during HIV infection is controversial. We investigated the frequency of TLR4 1063A/G and 1363C/T SNPs in 168 Omani donors [68 HIV-infected patients (>3% of Omani HIV-infected patients) and 100 healthy controls] and the association of these SNPs with the VL, CD8 and CD4 counts, and the immune recovery after cART as observed by CD4 T cell increase. SNPs were analyzed after the amplification of the regions that contain them by polymerase chain reaction (PCR) and sequencing of the PCR products. The TLR4 1063GG genotype was detected in the HIV-infected group only. No association was found between the studied SNPs and the average VL during 1 year of infection, the average CD4 and CD8 count during 1 year of viremia, the nadir CD4 count, the CD4 count when the patient reached VL <50 copies/mL due to cART, and the ratio of the CD4 count 3 and 6 months after reaching VL <50 copies/mL after cART to the last CD4 count before reaching VL <50 copies/mL. Our study suggests that TLR4 (1063A/G and 1363C/T) SNPs have no association with the VL or the CD4 and CD8 counts during HIV infection.

Original languageEnglish
Article number15027671
JournalGenetics and Molecular Research
Volume15
Issue number2
DOIs
Publication statusPublished - Apr 4 2016

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CD4 Lymphocyte Count
Viral Load
Single Nucleotide Polymorphism
Cell Count
HIV
T-Lymphocytes
Virus Diseases
Toll-Like Receptors
Infection
Polymerase Chain Reaction
Viremia
Innate Immunity
Disease Progression
Genotype
Tissue Donors

Keywords

  • CD4 T cell
  • CD8 T cell
  • HIV
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

@article{3f1b7c78f7df439f833a517368a07e5f,
title = "Frequency of TLR4 (1063A/G and 1363C/T) polymorphisms in healthy and HIV-infected Omani individuals and their relationship to viral load and T cell count",
abstract = "Toll-like receptors (TLRs) are essential elements of the innate immune response to different infections including the infection with human immunodeficiency virus (HIV). Single nucleotide polymorphisms (SNPs) in TLRs such as TLR4 1063A/G and 1363C/T have been found to be associated with changes in CD4 count, viral load (VL), and disease progression during HIV infection. However, the association of these SNPs with the pathogenesis during HIV infection is controversial. We investigated the frequency of TLR4 1063A/G and 1363C/T SNPs in 168 Omani donors [68 HIV-infected patients (>3{\%} of Omani HIV-infected patients) and 100 healthy controls] and the association of these SNPs with the VL, CD8 and CD4 counts, and the immune recovery after cART as observed by CD4 T cell increase. SNPs were analyzed after the amplification of the regions that contain them by polymerase chain reaction (PCR) and sequencing of the PCR products. The TLR4 1063GG genotype was detected in the HIV-infected group only. No association was found between the studied SNPs and the average VL during 1 year of infection, the average CD4 and CD8 count during 1 year of viremia, the nadir CD4 count, the CD4 count when the patient reached VL <50 copies/mL due to cART, and the ratio of the CD4 count 3 and 6 months after reaching VL <50 copies/mL after cART to the last CD4 count before reaching VL <50 copies/mL. Our study suggests that TLR4 (1063A/G and 1363C/T) SNPs have no association with the VL or the CD4 and CD8 counts during HIV infection.",
keywords = "CD4 T cell, CD8 T cell, HIV, Single nucleotide polymorphism",
author = "Said, {E. A.} and F. Al-Yafei and F. Zadjali and Al-Balushi, {M. S.} and Hasson, {S. S.} and Al-Mahroqi, {S. H.} and Koh, {C. Y.} and K. Al-Naamani and Al-Busaidi, {J. Z.} and Idris, {M. A.} and A. Balkhair and Al-Jabri, {A. A.}",
year = "2016",
month = "4",
day = "4",
doi = "10.4238/gmr.15027671",
language = "English",
volume = "15",
journal = "Genetics and Molecular Research",
issn = "1676-5680",
publisher = "Fundacao de Pesquisas Cientificas de Ribeirao Preto",
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TY - JOUR

T1 - Frequency of TLR4 (1063A/G and 1363C/T) polymorphisms in healthy and HIV-infected Omani individuals and their relationship to viral load and T cell count

AU - Said, E. A.

AU - Al-Yafei, F.

AU - Zadjali, F.

AU - Al-Balushi, M. S.

AU - Hasson, S. S.

AU - Al-Mahroqi, S. H.

AU - Koh, C. Y.

AU - Al-Naamani, K.

AU - Al-Busaidi, J. Z.

AU - Idris, M. A.

AU - Balkhair, A.

AU - Al-Jabri, A. A.

PY - 2016/4/4

Y1 - 2016/4/4

N2 - Toll-like receptors (TLRs) are essential elements of the innate immune response to different infections including the infection with human immunodeficiency virus (HIV). Single nucleotide polymorphisms (SNPs) in TLRs such as TLR4 1063A/G and 1363C/T have been found to be associated with changes in CD4 count, viral load (VL), and disease progression during HIV infection. However, the association of these SNPs with the pathogenesis during HIV infection is controversial. We investigated the frequency of TLR4 1063A/G and 1363C/T SNPs in 168 Omani donors [68 HIV-infected patients (>3% of Omani HIV-infected patients) and 100 healthy controls] and the association of these SNPs with the VL, CD8 and CD4 counts, and the immune recovery after cART as observed by CD4 T cell increase. SNPs were analyzed after the amplification of the regions that contain them by polymerase chain reaction (PCR) and sequencing of the PCR products. The TLR4 1063GG genotype was detected in the HIV-infected group only. No association was found between the studied SNPs and the average VL during 1 year of infection, the average CD4 and CD8 count during 1 year of viremia, the nadir CD4 count, the CD4 count when the patient reached VL <50 copies/mL due to cART, and the ratio of the CD4 count 3 and 6 months after reaching VL <50 copies/mL after cART to the last CD4 count before reaching VL <50 copies/mL. Our study suggests that TLR4 (1063A/G and 1363C/T) SNPs have no association with the VL or the CD4 and CD8 counts during HIV infection.

AB - Toll-like receptors (TLRs) are essential elements of the innate immune response to different infections including the infection with human immunodeficiency virus (HIV). Single nucleotide polymorphisms (SNPs) in TLRs such as TLR4 1063A/G and 1363C/T have been found to be associated with changes in CD4 count, viral load (VL), and disease progression during HIV infection. However, the association of these SNPs with the pathogenesis during HIV infection is controversial. We investigated the frequency of TLR4 1063A/G and 1363C/T SNPs in 168 Omani donors [68 HIV-infected patients (>3% of Omani HIV-infected patients) and 100 healthy controls] and the association of these SNPs with the VL, CD8 and CD4 counts, and the immune recovery after cART as observed by CD4 T cell increase. SNPs were analyzed after the amplification of the regions that contain them by polymerase chain reaction (PCR) and sequencing of the PCR products. The TLR4 1063GG genotype was detected in the HIV-infected group only. No association was found between the studied SNPs and the average VL during 1 year of infection, the average CD4 and CD8 count during 1 year of viremia, the nadir CD4 count, the CD4 count when the patient reached VL <50 copies/mL due to cART, and the ratio of the CD4 count 3 and 6 months after reaching VL <50 copies/mL after cART to the last CD4 count before reaching VL <50 copies/mL. Our study suggests that TLR4 (1063A/G and 1363C/T) SNPs have no association with the VL or the CD4 and CD8 counts during HIV infection.

KW - CD4 T cell

KW - CD8 T cell

KW - HIV

KW - Single nucleotide polymorphism

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