Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA

Heli Nummelin, Yasmin El Tahir, Pauli Ollikka, Mikael Skurnik, Adrian Goldman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype 0:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 Å, and diffract to 1.55 Å resolution on a synchrotron-radiation source.

Original languageEnglish
Pages (from-to)1042-1044
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number6 II
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Condensed Matter Physics

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