TY - JOUR
T1 - Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA
AU - Nummelin, Heli
AU - El Tahir, Yasmin
AU - Ollikka, Pauli
AU - Skurnik, Mikael
AU - Goldman, Adrian
PY - 2002
Y1 - 2002
N2 - A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype 0:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 Å, and diffract to 1.55 Å resolution on a synchrotron-radiation source.
AB - A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype 0:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 Å, and diffract to 1.55 Å resolution on a synchrotron-radiation source.
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U2 - 10.1107/S0907444902005231
DO - 10.1107/S0907444902005231
M3 - Article
C2 - 12037311
AN - SCOPUS:0036618133
VL - 58
SP - 1042
EP - 1044
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
SN - 0907-4449
IS - 6 II
ER -