Evaluation of Tomato Hybrids Carrying Ty-1 and Ty-2 Loci to Japanese Monopartite Begomovirus Species

Muhammad Shafiq Shahid, Takuya Ito, Junji Kimbara, Ayumu Onozato, Keiko T. Natsuaki, Masato Ikegami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tobacco leaf curl Japan virus, Honeysuckle yellow vein mosaic virus and Tomato yellow leaf curl virus are three begomoviruses that infect tomato crops in Japan. Tomato infection by begomoviruses has increased in Japan after the development of a high level of resistance to certain insecticides in some populations of the vector B. tabaci biotypes 'B and Q'. Ty-1 and Ty-2 homozygous tomato hybrids were evaluated for reaction to monopartite begomovirus species in Japan by Agrobacterium-mediated inoculation. Test plants were evaluated by a disease assessment scale (DAS), varying from 1 = no symptoms to 4 = severe symptoms, and systemic infection was evaluated by polymerase chain reaction (PCR), using specific begomovirus primers for each virus. Ty-1 hybrids showed tolerance to HYVMV and with a large number of plants being neither virus-free nor symptom-free. The response of Ty-1 hybrids was also resistant to moderately resistant against TbLCJV. The response of Ty-2 hybrids was resistant to highly resistant against the three monopartite begomoviruses, when compared with susceptible plants.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalJournal of Phytopathology
Volume161
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Begomovirus
Lycopersicon esculentum
Honeysuckle yellow vein mosaic virus
tomatoes
Tobacco leaf curl Japan virus
Japan
loci
signs and symptoms (plants)
Viruses
Lonicera
Tomato yellow leaf curl virus
Mosaic Viruses
viruses
Agrobacterium
Insecticides
Infection
biotypes
infection
Tobacco
Veins

Keywords

  • Begomovirus
  • Hybrids
  • Japan
  • Monopartite
  • Solanum lycopersicum

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Evaluation of Tomato Hybrids Carrying Ty-1 and Ty-2 Loci to Japanese Monopartite Begomovirus Species. / Shahid, Muhammad Shafiq; Ito, Takuya; Kimbara, Junji; Onozato, Ayumu; Natsuaki, Keiko T.; Ikegami, Masato.

In: Journal of Phytopathology, Vol. 161, No. 3, 03.2013, p. 205-209.

Research output: Contribution to journalArticle

Shahid, Muhammad Shafiq ; Ito, Takuya ; Kimbara, Junji ; Onozato, Ayumu ; Natsuaki, Keiko T. ; Ikegami, Masato. / Evaluation of Tomato Hybrids Carrying Ty-1 and Ty-2 Loci to Japanese Monopartite Begomovirus Species. In: Journal of Phytopathology. 2013 ; Vol. 161, No. 3. pp. 205-209.
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