TY - JOUR
T1 - Evaluation of a novel pentaplex microsatellite marker system for paternity studies in Atlantic cod (Gadus morhua L.)
AU - Wesmajervi, Mette Serine
AU - Westgaard, Jon Ivar
AU - Delghandi, Madjid
PY - 2006/9
Y1 - 2006/9
N2 - Five Atlantic cod (Gadus morhua L.) microsatellites (Gmo8, Gmo19, Gmo35, Gmo37 and Tch11) were selected for parentage assignment. All markers were co-amplified using dye-labelled primers and sized by automated sample processing in a 3130xl Genetic Analyser. The genotyping of samples was reproducible and alleles from all loci could be genotyped confidently (precision and accuracy above 99.7 and 99.1% respectively). The number of alleles, observed allelic and genotypic frequencies were calculated. A population database was established for fish originating from randomly selected wild-caught Norwegian coastal- and North-East Arctic cod. Combined paternity exclusion and the probability of match were 99.97 and 1.5 × 10-9 respectively. The multiplex assay system was used successfully for parental assignment of 2336 juveniles from different mass spawning breeding tanks. Of all offspring, 91.2% were assigned to a single parental pair. In total, 523 different matings were recorded, and 69 of 70 dams and all of the 30 sires contributed to the offspring.
AB - Five Atlantic cod (Gadus morhua L.) microsatellites (Gmo8, Gmo19, Gmo35, Gmo37 and Tch11) were selected for parentage assignment. All markers were co-amplified using dye-labelled primers and sized by automated sample processing in a 3130xl Genetic Analyser. The genotyping of samples was reproducible and alleles from all loci could be genotyped confidently (precision and accuracy above 99.7 and 99.1% respectively). The number of alleles, observed allelic and genotypic frequencies were calculated. A population database was established for fish originating from randomly selected wild-caught Norwegian coastal- and North-East Arctic cod. Combined paternity exclusion and the probability of match were 99.97 and 1.5 × 10-9 respectively. The multiplex assay system was used successfully for parental assignment of 2336 juveniles from different mass spawning breeding tanks. Of all offspring, 91.2% were assigned to a single parental pair. In total, 523 different matings were recorded, and 69 of 70 dams and all of the 30 sires contributed to the offspring.
KW - Atlantic cod
KW - Genotyping
KW - Microsatellite markers
KW - Multiplexing
KW - Parental assignment
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U2 - 10.1111/j.1365-2109.2006.01549.x
DO - 10.1111/j.1365-2109.2006.01549.x
M3 - Article
AN - SCOPUS:33747610953
SN - 1355-557X
VL - 37
SP - 1195
EP - 1201
JO - Aquaculture Research
JF - Aquaculture Research
IS - 12
ER -