Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library

Mette Serine Wesmajervi, Tekle Tafese, Jørgen Stenvik, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.

Original languageEnglish
Pages (from-to)138-140
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Gadus morhua
Genomic Library
genomic libraries
disequilibrium
heterozygosity
Microsatellite Repeats
clone
genomics
allele
microsatellite repeats
loci
Genetic Linkage
Linkage Disequilibrium
Homozygote
fish
Fish
Fishes
Clone Cells
Alleles
linkage disequilibrium

Keywords

  • Atlantic cod
  • Enriched genomic library
  • Microsatellites
  • Type II markers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

Cite this

Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library. / Wesmajervi, Mette Serine; Tafese, Tekle; Stenvik, Jørgen; Fjalestad, Kjersti Turid; Damsgård, Børge; Delghandi, Madjid.

In: Molecular Ecology Notes, Vol. 7, No. 1, 01.2007, p. 138-140.

Research output: Contribution to journalArticle

Wesmajervi, Mette Serine ; Tafese, Tekle ; Stenvik, Jørgen ; Fjalestad, Kjersti Turid ; Damsgård, Børge ; Delghandi, Madjid. / Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library. In: Molecular Ecology Notes. 2007 ; Vol. 7, No. 1. pp. 138-140.
@article{20ed49170ec24f6a9b932b84d6b0f496,
title = "Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library",
abstract = "One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.",
keywords = "Atlantic cod, Enriched genomic library, Microsatellites, Type II markers",
author = "Wesmajervi, {Mette Serine} and Tekle Tafese and J{\o}rgen Stenvik and Fjalestad, {Kjersti Turid} and B{\o}rge Damsg{\aa}rd and Madjid Delghandi",
year = "2007",
month = "1",
doi = "10.1111/j.1471-8286.2006.01555.x",
language = "English",
volume = "7",
pages = "138--140",
journal = "Molecular Ecology Resources",
issn = "1755-098X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library

AU - Wesmajervi, Mette Serine

AU - Tafese, Tekle

AU - Stenvik, Jørgen

AU - Fjalestad, Kjersti Turid

AU - Damsgård, Børge

AU - Delghandi, Madjid

PY - 2007/1

Y1 - 2007/1

N2 - One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.

AB - One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.

KW - Atlantic cod

KW - Enriched genomic library

KW - Microsatellites

KW - Type II markers

UR - http://www.scopus.com/inward/record.url?scp=33845991140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845991140&partnerID=8YFLogxK

U2 - 10.1111/j.1471-8286.2006.01555.x

DO - 10.1111/j.1471-8286.2006.01555.x

M3 - Article

AN - SCOPUS:33845991140

VL - 7

SP - 138

EP - 140

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

SN - 1755-098X

IS - 1

ER -