Eight new microsatellite markers in Atlantic cod (Gadus morhua L.) derived from an enriched genomic library

Mette Serine Wesmajervi, Tekle Tafese, Jørgen Stenvik, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

One hundred and fifty random clones from an enriched genomic library of Atlantic cod were sequenced. Primer pairs were designed for 15 microsatellites containing perfect di-, tri-, tetra- and hexanucleotide repeats and characterized in 96 unrelated fish. Eight markers were successfully amplified, with the number of alleles ranging from two to nine per locus and observed heterozygosity ranging from 0.341 to 0.977. Loci Gmo-G13 and Gmo-G14 had a significant excess of homozygotes. All loci conformed to the Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed no significant departure from the null hypothesis between any of the loci.

Original languageEnglish
Pages (from-to)138-140
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Atlantic cod
  • Enriched genomic library
  • Microsatellites
  • Type II markers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

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