Differential induction of peroxidase and polyphenol oxidase isozymes in suspension-cultured cells of blast resistant and susceptible genotypes of rice in response to treatment with Magnaporthe grisea elicitor and toxin

S. Malathi, S. Madhavan, R. Rabindran, V. Paranidharan, R. Velazhahan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of elicitor from mycelial walls of Magnaporthe grisea, the rice blast fungus and α-picolinic acid, one of the toxins produced by M. grisea on induction of peroxidase (PO), polyphenol oxidase (PPO) in suspension-cultured rice (Oryza sativa L.) cells was studied. Cultured cells of blast resistant (Usen) and susceptible (CO39) rice genotypes were treated with elicitor (50 μg of glucose equivalents per ml) or α-picolinic acid (400 ppm). The cells were harvested at different time intervals and analysed for the induction of PO and PPO. PO isozyme analysis indicated that the elicitor strongly induced the activities of PO-2 and PO-3 in cultured cells of Usen 3 days after treatment. In Usen, toxin also induced the activities of PO-3 and PO-4. However, similar levels of activities corresponding to these isozymes were recorded 7 days after treatment. In CO39, the activities of PO-1 and PO-2 were induced 3 days after elicitor treatment. In contrast, the toxin suppressed the activity of PO-2. The elicitor induced the activities of PPO-1, PPO-2 and PPO-3 in both Usen and CO39. In Usen, steady increase of PPO-3 was observed and higher level of activity was recorded 5 days after treatment. In CO39, higher level of PPO-3 was observed 1 day after treatment and declined thereafter. However, the activities of PPO-1 and PPO-2 increased 3 days after treatment in CO39. In the toxin-treated cells of Usen, higher level of activity of PPO-3 was observed 3 days after treatment.

Original languageEnglish
Pages (from-to)2524-2532
Number of pages9
JournalArchives of Phytopathology and Plant Protection
Volume47
Issue number20
DOIs
Publication statusPublished - 2014

Fingerprint

Magnaporthe grisea
catechol oxidase
cultured cells
isozymes
peroxidase
toxins
rice
genotype
picolinic acid
elicitors
blast disease
cells
Oryza sativa

Keywords

  • elicitor
  • Oryza sativa
  • peroxidase
  • polyphenol oxidase
  • toxin

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Differential induction of peroxidase and polyphenol oxidase isozymes in suspension-cultured cells of blast resistant and susceptible genotypes of rice in response to treatment with Magnaporthe grisea elicitor and toxin",
abstract = "The effect of elicitor from mycelial walls of Magnaporthe grisea, the rice blast fungus and α-picolinic acid, one of the toxins produced by M. grisea on induction of peroxidase (PO), polyphenol oxidase (PPO) in suspension-cultured rice (Oryza sativa L.) cells was studied. Cultured cells of blast resistant (Usen) and susceptible (CO39) rice genotypes were treated with elicitor (50 μg of glucose equivalents per ml) or α-picolinic acid (400 ppm). The cells were harvested at different time intervals and analysed for the induction of PO and PPO. PO isozyme analysis indicated that the elicitor strongly induced the activities of PO-2 and PO-3 in cultured cells of Usen 3 days after treatment. In Usen, toxin also induced the activities of PO-3 and PO-4. However, similar levels of activities corresponding to these isozymes were recorded 7 days after treatment. In CO39, the activities of PO-1 and PO-2 were induced 3 days after elicitor treatment. In contrast, the toxin suppressed the activity of PO-2. The elicitor induced the activities of PPO-1, PPO-2 and PPO-3 in both Usen and CO39. In Usen, steady increase of PPO-3 was observed and higher level of activity was recorded 5 days after treatment. In CO39, higher level of PPO-3 was observed 1 day after treatment and declined thereafter. However, the activities of PPO-1 and PPO-2 increased 3 days after treatment in CO39. In the toxin-treated cells of Usen, higher level of activity of PPO-3 was observed 3 days after treatment.",
keywords = "elicitor, Oryza sativa, peroxidase, polyphenol oxidase, toxin",
author = "S. Malathi and S. Madhavan and R. Rabindran and V. Paranidharan and R. Velazhahan",
year = "2014",
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TY - JOUR

T1 - Differential induction of peroxidase and polyphenol oxidase isozymes in suspension-cultured cells of blast resistant and susceptible genotypes of rice in response to treatment with Magnaporthe grisea elicitor and toxin

AU - Malathi, S.

AU - Madhavan, S.

AU - Rabindran, R.

AU - Paranidharan, V.

AU - Velazhahan, R.

PY - 2014

Y1 - 2014

N2 - The effect of elicitor from mycelial walls of Magnaporthe grisea, the rice blast fungus and α-picolinic acid, one of the toxins produced by M. grisea on induction of peroxidase (PO), polyphenol oxidase (PPO) in suspension-cultured rice (Oryza sativa L.) cells was studied. Cultured cells of blast resistant (Usen) and susceptible (CO39) rice genotypes were treated with elicitor (50 μg of glucose equivalents per ml) or α-picolinic acid (400 ppm). The cells were harvested at different time intervals and analysed for the induction of PO and PPO. PO isozyme analysis indicated that the elicitor strongly induced the activities of PO-2 and PO-3 in cultured cells of Usen 3 days after treatment. In Usen, toxin also induced the activities of PO-3 and PO-4. However, similar levels of activities corresponding to these isozymes were recorded 7 days after treatment. In CO39, the activities of PO-1 and PO-2 were induced 3 days after elicitor treatment. In contrast, the toxin suppressed the activity of PO-2. The elicitor induced the activities of PPO-1, PPO-2 and PPO-3 in both Usen and CO39. In Usen, steady increase of PPO-3 was observed and higher level of activity was recorded 5 days after treatment. In CO39, higher level of PPO-3 was observed 1 day after treatment and declined thereafter. However, the activities of PPO-1 and PPO-2 increased 3 days after treatment in CO39. In the toxin-treated cells of Usen, higher level of activity of PPO-3 was observed 3 days after treatment.

AB - The effect of elicitor from mycelial walls of Magnaporthe grisea, the rice blast fungus and α-picolinic acid, one of the toxins produced by M. grisea on induction of peroxidase (PO), polyphenol oxidase (PPO) in suspension-cultured rice (Oryza sativa L.) cells was studied. Cultured cells of blast resistant (Usen) and susceptible (CO39) rice genotypes were treated with elicitor (50 μg of glucose equivalents per ml) or α-picolinic acid (400 ppm). The cells were harvested at different time intervals and analysed for the induction of PO and PPO. PO isozyme analysis indicated that the elicitor strongly induced the activities of PO-2 and PO-3 in cultured cells of Usen 3 days after treatment. In Usen, toxin also induced the activities of PO-3 and PO-4. However, similar levels of activities corresponding to these isozymes were recorded 7 days after treatment. In CO39, the activities of PO-1 and PO-2 were induced 3 days after elicitor treatment. In contrast, the toxin suppressed the activity of PO-2. The elicitor induced the activities of PPO-1, PPO-2 and PPO-3 in both Usen and CO39. In Usen, steady increase of PPO-3 was observed and higher level of activity was recorded 5 days after treatment. In CO39, higher level of PPO-3 was observed 1 day after treatment and declined thereafter. However, the activities of PPO-1 and PPO-2 increased 3 days after treatment in CO39. In the toxin-treated cells of Usen, higher level of activity of PPO-3 was observed 3 days after treatment.

KW - elicitor

KW - Oryza sativa

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KW - polyphenol oxidase

KW - toxin

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