Differential antibody recognition of FC27-like Plasmodium falciparum merozoite surface protein MSP2 antigens which lack 12 amino acid repeats

Lisa C. Ranford-Cartwright, Rachel R. Taylor, Nima Asgari-Jirhandeh, Donald B. Smith, Paul E. Roberts, V. Jane Robinson, Hamza A. Babiker, Eleanor M. Riley, David Walliker, Jana S. Mcbride

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Three alleles of the FC27-type allelic family of the MSP2 gene of the malaria parasite Plasmodium falciparum have been sequenced from parasites from the field (The Gambia and Tanzania). These alleles lack the 12 amino acid repeat units which are usual in this family of MSP2 alleles. We have investigated the recognition by sera from an endemic area (The Gambia) of three recombinant MSP2 proteins that have 5, 1 and no copies of this repeat region. Antibody recognition of these recombinant proteins varied according to the number of repeats present. High titre antibody levels were seen with most sera using the recombinant protein with 5 x 12-mer repeats, whereas only low responses were measured using proteins containing 1 or no 12-mer repeats. Several sera entirely failed to recognise the protein which lacked 12-mer repeats. The data suggest that variation in the number of tandem repeat sequences could allow the parasite to avoid high avidity antibody binding and this may allow escape from immune recognition.

Original languageEnglish
Pages (from-to)411-420
Number of pages10
JournalParasite Immunology
Volume18
Issue number8
Publication statusPublished - 1996

Fingerprint

Merozoites
Plasmodium falciparum
Recombinant Proteins
Gambia
Membrane Proteins
Parasites
Alleles
Antigens
Amino Acids
Antibodies
Serum
Tandem Repeat Sequences
Antibody Affinity
Tanzania
Falciparum Malaria
Proteins
Genes

Keywords

  • Antibody reponse
  • Malaria
  • MSP2
  • Plasmodium falciparum
  • Repetitive epitope

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Ranford-Cartwright, L. C., Taylor, R. R., Asgari-Jirhandeh, N., Smith, D. B., Roberts, P. E., Robinson, V. J., ... Mcbride, J. S. (1996). Differential antibody recognition of FC27-like Plasmodium falciparum merozoite surface protein MSP2 antigens which lack 12 amino acid repeats. Parasite Immunology, 18(8), 411-420.

Differential antibody recognition of FC27-like Plasmodium falciparum merozoite surface protein MSP2 antigens which lack 12 amino acid repeats. / Ranford-Cartwright, Lisa C.; Taylor, Rachel R.; Asgari-Jirhandeh, Nima; Smith, Donald B.; Roberts, Paul E.; Robinson, V. Jane; Babiker, Hamza A.; Riley, Eleanor M.; Walliker, David; Mcbride, Jana S.

In: Parasite Immunology, Vol. 18, No. 8, 1996, p. 411-420.

Research output: Contribution to journalArticle

Ranford-Cartwright, LC, Taylor, RR, Asgari-Jirhandeh, N, Smith, DB, Roberts, PE, Robinson, VJ, Babiker, HA, Riley, EM, Walliker, D & Mcbride, JS 1996, 'Differential antibody recognition of FC27-like Plasmodium falciparum merozoite surface protein MSP2 antigens which lack 12 amino acid repeats', Parasite Immunology, vol. 18, no. 8, pp. 411-420.
Ranford-Cartwright LC, Taylor RR, Asgari-Jirhandeh N, Smith DB, Roberts PE, Robinson VJ et al. Differential antibody recognition of FC27-like Plasmodium falciparum merozoite surface protein MSP2 antigens which lack 12 amino acid repeats. Parasite Immunology. 1996;18(8):411-420.
Ranford-Cartwright, Lisa C. ; Taylor, Rachel R. ; Asgari-Jirhandeh, Nima ; Smith, Donald B. ; Roberts, Paul E. ; Robinson, V. Jane ; Babiker, Hamza A. ; Riley, Eleanor M. ; Walliker, David ; Mcbride, Jana S. / Differential antibody recognition of FC27-like Plasmodium falciparum merozoite surface protein MSP2 antigens which lack 12 amino acid repeats. In: Parasite Immunology. 1996 ; Vol. 18, No. 8. pp. 411-420.
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