Dietary folate protects against azoxymethane-induced aberrant crypt foci development and oxidative stress in rat colon

Khalid S. Al-Numair, Mostafa I. Waly, Amanat Ali, Mohamed M. Essa, Mohamed F. Farhat, Mohamed A. Alsaif

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Azoxymethane (AOM) induces cancer and oxidative stress in rat colon. This study tested the hypothesis that dietary folate supplementation protects against AOM-induced oxidative stress and reduces aberrant crypt foci (ACF) development in rat colon. Fifty-four weanling male albino rats, with an average body weight of 50+5 g, were randomly divided into three groups-A, B and C (18 rats per group)-and fed 2, 8 or 40 mg of folic acid per kg of supplemented diets, respectively, throughout the eight weeks' experimental period. The animals were supplied with diet and water ad libitum for four weeks and they reached an average body weight of 100 g. Thereafter each group was then further randomly subdivided into three subgroups (six rats per subgroup): control, vehicle and AOM-injected groups. The control group did not receive any treatment (neither AOM injection nor saline), the rats in the vehicle group were given 1 mL intraperitoneal injection of saline once a week for two weeks and the rats in the AOM-injected group were given two intraperitoneal injections of AOM dissolved in saline once a week for two weeks totaling 30 mg/kg body weight. After the last AOM injection, animals were continuously fed ad libitum their specified diet for two weeks of last AOM injection, all rats were sacrificed, and colon tissues were collected and used for ACF enumeration and measurements of glutathione (GSH) and total antioxidant capacity (TAC). The results revealed that AOM-injected rats showed lower levels of GSH and TAC as compared with control and vehicle groups. Folic acid-supplemented diets suppressed the AOM-induced ACF and GSH depletion in a dose-dependent manner and augmented the TAC. It was concluded that folic acid supplementation protects against the AOM-induced ACF formation by suppressing the AOM-induced GSH depletion in rat colon cells.

Original languageEnglish
Pages (from-to)1005-1011
Number of pages7
JournalExperimental Biology and Medicine
Volume236
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Aberrant Crypt Foci
Azoxymethane
Oxidative stress
Folic Acid
Rats
Colon
Oxidative Stress
Nutrition
Diet
Antioxidants
Body Weight
Intraperitoneal Injections
Injections
Animals
Control Groups
Dietary Supplements
Glutathione

Keywords

  • Azoxymethane
  • Colon cancer
  • Folate
  • Glutathione
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dietary folate protects against azoxymethane-induced aberrant crypt foci development and oxidative stress in rat colon. / Al-Numair, Khalid S.; Waly, Mostafa I.; Ali, Amanat; Essa, Mohamed M.; Farhat, Mohamed F.; Alsaif, Mohamed A.

In: Experimental Biology and Medicine, Vol. 236, No. 9, 09.2011, p. 1005-1011.

Research output: Contribution to journalArticle

@article{e131b85c477a4862a1f7cbb82b488035,
title = "Dietary folate protects against azoxymethane-induced aberrant crypt foci development and oxidative stress in rat colon",
abstract = "Azoxymethane (AOM) induces cancer and oxidative stress in rat colon. This study tested the hypothesis that dietary folate supplementation protects against AOM-induced oxidative stress and reduces aberrant crypt foci (ACF) development in rat colon. Fifty-four weanling male albino rats, with an average body weight of 50+5 g, were randomly divided into three groups-A, B and C (18 rats per group)-and fed 2, 8 or 40 mg of folic acid per kg of supplemented diets, respectively, throughout the eight weeks' experimental period. The animals were supplied with diet and water ad libitum for four weeks and they reached an average body weight of 100 g. Thereafter each group was then further randomly subdivided into three subgroups (six rats per subgroup): control, vehicle and AOM-injected groups. The control group did not receive any treatment (neither AOM injection nor saline), the rats in the vehicle group were given 1 mL intraperitoneal injection of saline once a week for two weeks and the rats in the AOM-injected group were given two intraperitoneal injections of AOM dissolved in saline once a week for two weeks totaling 30 mg/kg body weight. After the last AOM injection, animals were continuously fed ad libitum their specified diet for two weeks of last AOM injection, all rats were sacrificed, and colon tissues were collected and used for ACF enumeration and measurements of glutathione (GSH) and total antioxidant capacity (TAC). The results revealed that AOM-injected rats showed lower levels of GSH and TAC as compared with control and vehicle groups. Folic acid-supplemented diets suppressed the AOM-induced ACF and GSH depletion in a dose-dependent manner and augmented the TAC. It was concluded that folic acid supplementation protects against the AOM-induced ACF formation by suppressing the AOM-induced GSH depletion in rat colon cells.",
keywords = "Azoxymethane, Colon cancer, Folate, Glutathione, Oxidative stress",
author = "Al-Numair, {Khalid S.} and Waly, {Mostafa I.} and Amanat Ali and Essa, {Mohamed M.} and Farhat, {Mohamed F.} and Alsaif, {Mohamed A.}",
year = "2011",
month = "9",
doi = "10.1258/ebm.2011.011010",
language = "English",
volume = "236",
pages = "1005--1011",
journal = "Experimental Biology and Medicine",
issn = "1535-3702",
publisher = "SAGE Publications Ltd",
number = "9",

}

TY - JOUR

T1 - Dietary folate protects against azoxymethane-induced aberrant crypt foci development and oxidative stress in rat colon

AU - Al-Numair, Khalid S.

AU - Waly, Mostafa I.

AU - Ali, Amanat

AU - Essa, Mohamed M.

AU - Farhat, Mohamed F.

AU - Alsaif, Mohamed A.

PY - 2011/9

Y1 - 2011/9

N2 - Azoxymethane (AOM) induces cancer and oxidative stress in rat colon. This study tested the hypothesis that dietary folate supplementation protects against AOM-induced oxidative stress and reduces aberrant crypt foci (ACF) development in rat colon. Fifty-four weanling male albino rats, with an average body weight of 50+5 g, were randomly divided into three groups-A, B and C (18 rats per group)-and fed 2, 8 or 40 mg of folic acid per kg of supplemented diets, respectively, throughout the eight weeks' experimental period. The animals were supplied with diet and water ad libitum for four weeks and they reached an average body weight of 100 g. Thereafter each group was then further randomly subdivided into three subgroups (six rats per subgroup): control, vehicle and AOM-injected groups. The control group did not receive any treatment (neither AOM injection nor saline), the rats in the vehicle group were given 1 mL intraperitoneal injection of saline once a week for two weeks and the rats in the AOM-injected group were given two intraperitoneal injections of AOM dissolved in saline once a week for two weeks totaling 30 mg/kg body weight. After the last AOM injection, animals were continuously fed ad libitum their specified diet for two weeks of last AOM injection, all rats were sacrificed, and colon tissues were collected and used for ACF enumeration and measurements of glutathione (GSH) and total antioxidant capacity (TAC). The results revealed that AOM-injected rats showed lower levels of GSH and TAC as compared with control and vehicle groups. Folic acid-supplemented diets suppressed the AOM-induced ACF and GSH depletion in a dose-dependent manner and augmented the TAC. It was concluded that folic acid supplementation protects against the AOM-induced ACF formation by suppressing the AOM-induced GSH depletion in rat colon cells.

AB - Azoxymethane (AOM) induces cancer and oxidative stress in rat colon. This study tested the hypothesis that dietary folate supplementation protects against AOM-induced oxidative stress and reduces aberrant crypt foci (ACF) development in rat colon. Fifty-four weanling male albino rats, with an average body weight of 50+5 g, were randomly divided into three groups-A, B and C (18 rats per group)-and fed 2, 8 or 40 mg of folic acid per kg of supplemented diets, respectively, throughout the eight weeks' experimental period. The animals were supplied with diet and water ad libitum for four weeks and they reached an average body weight of 100 g. Thereafter each group was then further randomly subdivided into three subgroups (six rats per subgroup): control, vehicle and AOM-injected groups. The control group did not receive any treatment (neither AOM injection nor saline), the rats in the vehicle group were given 1 mL intraperitoneal injection of saline once a week for two weeks and the rats in the AOM-injected group were given two intraperitoneal injections of AOM dissolved in saline once a week for two weeks totaling 30 mg/kg body weight. After the last AOM injection, animals were continuously fed ad libitum their specified diet for two weeks of last AOM injection, all rats were sacrificed, and colon tissues were collected and used for ACF enumeration and measurements of glutathione (GSH) and total antioxidant capacity (TAC). The results revealed that AOM-injected rats showed lower levels of GSH and TAC as compared with control and vehicle groups. Folic acid-supplemented diets suppressed the AOM-induced ACF and GSH depletion in a dose-dependent manner and augmented the TAC. It was concluded that folic acid supplementation protects against the AOM-induced ACF formation by suppressing the AOM-induced GSH depletion in rat colon cells.

KW - Azoxymethane

KW - Colon cancer

KW - Folate

KW - Glutathione

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=80052465077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052465077&partnerID=8YFLogxK

U2 - 10.1258/ebm.2011.011010

DO - 10.1258/ebm.2011.011010

M3 - Article

C2 - 21768165

AN - SCOPUS:80052465077

VL - 236

SP - 1005

EP - 1011

JO - Experimental Biology and Medicine

JF - Experimental Biology and Medicine

SN - 1535-3702

IS - 9

ER -