Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)

Jon Ivar Westgaard, Tekle Tafese, Mette Serine Wesmajervi, Frank Nilsen, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi

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7 Citations (Scopus)

Abstract

Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

Original languageEnglish
Pages (from-to)1503-1506
Number of pages4
JournalConservation Genetics
Volume8
Issue number6
DOIs
Publication statusPublished - Dec 2007

Keywords

  • Atlantic cod
  • Expressed sequence tag
  • Microsatellite
  • Type I marker

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)
  • Nature and Landscape Conservation

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    Westgaard, J. I., Tafese, T., Wesmajervi, M. S., Nilsen, F., Fjalestad, K. T., Damsgård, B., & Delghandi, M. (2007). Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.). Conservation Genetics, 8(6), 1503-1506. https://doi.org/10.1007/s10592-007-9283-5