TY - JOUR
T1 - Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)
AU - Westgaard, Jon Ivar
AU - Tafese, Tekle
AU - Wesmajervi, Mette Serine
AU - Nilsen, Frank
AU - Fjalestad, Kjersti Turid
AU - Damsgård, Børge
AU - Delghandi, Madjid
N1 - Funding Information:
Acknowledgments The present study was supported by the National Programme for Research in Functional Genomics in Norway (FUGE) within the Research Council of Norway (no. 162486/S10), by the Research Council of Norway (no. 164981/S40) and by the EU project SEAFOODplus (no. 506359). We thank Dr. Marie Cooper for reading of the manuscript and making helpful suggestions.
PY - 2007/12
Y1 - 2007/12
N2 - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.
AB - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.
KW - Atlantic cod
KW - Expressed sequence tag
KW - Microsatellite
KW - Type I marker
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U2 - 10.1007/s10592-007-9283-5
DO - 10.1007/s10592-007-9283-5
M3 - Article
AN - SCOPUS:36148938335
VL - 8
SP - 1503
EP - 1506
JO - Conservation Genetics
JF - Conservation Genetics
SN - 1566-0621
IS - 6
ER -