Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)

Jon Ivar Westgaard; Tekle Tafese; Mette Serine Wesmajervi; Frank Nilsen; Kjersti Turid Fjalestad; Børge Damsgård; Madjid Delghandi

doi:10.1007/s10592-007-9283-5

Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)

Jon Ivar Westgaard, Tekle Tafese, Mette Serine Wesmajervi, Frank Nilsen, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi^*

^*Corresponding author for this work

Excellence in Marine Biotechnology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

Original language	English
Pages (from-to)	1503-1506
Number of pages	4
Journal	Conservation Genetics
Volume	8
Issue number	6
DOIs	https://doi.org/10.1007/s10592-007-9283-5
Publication status	Published - Dec 2007

Keywords

Atlantic cod
Expressed sequence tag
Microsatellite
Type I marker

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Genetics
Genetics(clinical)
Nature and Landscape Conservation

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title = "Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)",

abstract = "Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.",

keywords = "Atlantic cod, Expressed sequence tag, Microsatellite, Type I marker",

author = "Westgaard, {Jon Ivar} and Tekle Tafese and Wesmajervi, {Mette Serine} and Frank Nilsen and Fjalestad, {Kjersti Turid} and B{\o}rge Damsg{\aa}rd and Madjid Delghandi",

year = "2007",

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AU - Westgaard, Jon Ivar

AU - Tafese, Tekle

AU - Wesmajervi, Mette Serine

AU - Nilsen, Frank

AU - Fjalestad, Kjersti Turid

AU - Damsgård, Børge

AU - Delghandi, Madjid

PY - 2007/12

Y1 - 2007/12

N2 - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

AB - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

KW - Atlantic cod

KW - Expressed sequence tag

KW - Microsatellite

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