Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)

Jon Ivar Westgaard, Tekle Tafese, Mette Serine Wesmajervi, Frank Nilsen, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

Original languageEnglish
Pages (from-to)1503-1506
Number of pages4
JournalConservation Genetics
Volume8
Issue number6
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Gadus morhua
Expressed Sequence Tags
expressed sequence tags
Microsatellite Repeats
microsatellite repeats
loci
Genetic Linkage
Linkage Disequilibrium
disequilibrium
heterozygosity
allele
Fishes
Alleles
linkage disequilibrium
gene
fish
Genes
marker
alleles
genes

Keywords

  • Atlantic cod
  • Expressed sequence tag
  • Microsatellite
  • Type I marker

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)
  • Nature and Landscape Conservation

Cite this

Westgaard, J. I., Tafese, T., Wesmajervi, M. S., Nilsen, F., Fjalestad, K. T., Damsgård, B., & Delghandi, M. (2007). Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.). Conservation Genetics, 8(6), 1503-1506. https://doi.org/10.1007/s10592-007-9283-5

Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.). / Westgaard, Jon Ivar; Tafese, Tekle; Wesmajervi, Mette Serine; Nilsen, Frank; Fjalestad, Kjersti Turid; Damsgård, Børge; Delghandi, Madjid.

In: Conservation Genetics, Vol. 8, No. 6, 12.2007, p. 1503-1506.

Research output: Contribution to journalArticle

Westgaard, JI, Tafese, T, Wesmajervi, MS, Nilsen, F, Fjalestad, KT, Damsgård, B & Delghandi, M 2007, 'Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)', Conservation Genetics, vol. 8, no. 6, pp. 1503-1506. https://doi.org/10.1007/s10592-007-9283-5
Westgaard JI, Tafese T, Wesmajervi MS, Nilsen F, Fjalestad KT, Damsgård B et al. Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.). Conservation Genetics. 2007 Dec;8(6):1503-1506. https://doi.org/10.1007/s10592-007-9283-5
Westgaard, Jon Ivar ; Tafese, Tekle ; Wesmajervi, Mette Serine ; Nilsen, Frank ; Fjalestad, Kjersti Turid ; Damsgård, Børge ; Delghandi, Madjid. / Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.). In: Conservation Genetics. 2007 ; Vol. 8, No. 6. pp. 1503-1506.
@article{d5c3ece5c2e649708fab9f6c5dd72eca,
title = "Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)",
abstract = "Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.",
keywords = "Atlantic cod, Expressed sequence tag, Microsatellite, Type I marker",
author = "Westgaard, {Jon Ivar} and Tekle Tafese and Wesmajervi, {Mette Serine} and Frank Nilsen and Fjalestad, {Kjersti Turid} and B{\o}rge Damsg{\aa}rd and Madjid Delghandi",
year = "2007",
month = "12",
doi = "10.1007/s10592-007-9283-5",
language = "English",
volume = "8",
pages = "1503--1506",
journal = "Conservation Genetics",
issn = "1566-0621",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)

AU - Westgaard, Jon Ivar

AU - Tafese, Tekle

AU - Wesmajervi, Mette Serine

AU - Nilsen, Frank

AU - Fjalestad, Kjersti Turid

AU - Damsgård, Børge

AU - Delghandi, Madjid

PY - 2007/12

Y1 - 2007/12

N2 - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

AB - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.

KW - Atlantic cod

KW - Expressed sequence tag

KW - Microsatellite

KW - Type I marker

UR - http://www.scopus.com/inward/record.url?scp=36148938335&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36148938335&partnerID=8YFLogxK

U2 - 10.1007/s10592-007-9283-5

DO - 10.1007/s10592-007-9283-5

M3 - Article

VL - 8

SP - 1503

EP - 1506

JO - Conservation Genetics

JF - Conservation Genetics

SN - 1566-0621

IS - 6

ER -