Abstract
Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.
| Original language | English |
|---|---|
| Pages (from-to) | 1503-1506 |
| Number of pages | 4 |
| Journal | Conservation Genetics |
| Volume | 8 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2007 |
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Keywords
- Atlantic cod
- Expressed sequence tag
- Microsatellite
- Type I marker
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Genetics
- Genetics(clinical)
- Nature and Landscape Conservation
Cite this
Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.). / Westgaard, Jon Ivar; Tafese, Tekle; Wesmajervi, Mette Serine; Nilsen, Frank; Fjalestad, Kjersti Turid; Damsgård, Børge; Delghandi, Madjid.
In: Conservation Genetics, Vol. 8, No. 6, 12.2007, p. 1503-1506.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of ten new EST-derived microsatellites in Atlantic cod (Gadus morhua L.)
AU - Westgaard, Jon Ivar
AU - Tafese, Tekle
AU - Wesmajervi, Mette Serine
AU - Nilsen, Frank
AU - Fjalestad, Kjersti Turid
AU - Damsgård, Børge
AU - Delghandi, Madjid
PY - 2007/12
Y1 - 2007/12
N2 - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.
AB - Ten polymorphic microsatellite markers were developed from approximately 1,300 expressed sequence tags (ESTs) of Atlantic cod (Gadus morhua L.). Thirty two primer pairs were designed for EST sequences containing perfect di- tri- tetra- and pentanucleotide motifs and characterised in 96 unrelated fish. Ten markers were successfully amplified with number of alleles from 2 to 13 per locus and observed and expected heterozygosity ranging from 0.03 to 0.69 and 0.03 to 0.74, respectively. Loci Gmo-C131, C132 and C136 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C131 and Gmo-C132 and C128 and Gmo-C133. The gene identity was determined at five of the loci, confirming the associated microsatellites as Type I markers. The new microsatellites reported in this work can be used for conservation and enhancement of wild stocks for commercial harvesting.
KW - Atlantic cod
KW - Expressed sequence tag
KW - Microsatellite
KW - Type I marker
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U2 - 10.1007/s10592-007-9283-5
DO - 10.1007/s10592-007-9283-5
M3 - Article
VL - 8
SP - 1503
EP - 1506
JO - Conservation Genetics
JF - Conservation Genetics
SN - 1566-0621
IS - 6
ER -