Development of formulations of Trichoderma harzianum strain M1 for control of damping-off of tomato caused by Pythium aphanidermatum

J. Jayaraj, N. V. Radhakrishnan, R. Velazhahan

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A carbendazim resistant Trichoderma harzianum strain M1, inhibitory to the growth of the damping-off pathogen Pythium aphanidermatum was used for the development of new carrier formulations. Seven different formulations (talc, lignite, lignite + fly ash-based powder formulation, wettable powder, bentonite paste, polyethylene glycol-paste and gelatin-glycerin-gel) were developed for seed treatment. Shelf life of the formulations was evaluated under storage at 24°C up to nine months. The population of propagules was optimum in all the formulations up to three months of storage. Seed treatment with Trichoderma formulations reduced the incidence of damping-off disease of tomato up to 74% and also enhanced the plant biomass under greenhouse and field conditions. Active colonization of T. harzianum in the rhizosphere of tomato plants was observed following seed treatment with the formulations.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalArchives of Phytopathology and Plant Protection
Issue number1
Publication statusPublished - Feb 1 2006



  • Biological control
  • Damping-off
  • Formulations
  • Lycopersicon esculentum
  • Pythium aphanidermatum
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

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