Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill - Structural analysis and biological toxicity of degradation product of aflatoxin G1

Rethinasamy Velazhahan, Selvaraj Vijayanandraj, Ayyathurai Vijayasamundeeswari, Vaikuntavasen Paranidharan, Ramasamy Samiyappan, Takeo Iwamoto, Bernd Friebe, Subbaratnam Muthukrishnan

Research output: Contribution to journalArticle

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Abstract

Aqueous extracts obtained from leaves/seeds of various medicinal plants were evaluated for their ability to detoxify aflatoxin G1 (AFG1) by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Of the various plant extracts, the seed extract of Ajowan (Trachyspermum ammi (L.) Sprague ex Turrill) showed the maximum degradation of AFG1 up to 65%. The dialyzed T. ammi extract was more effective than the crude extract, capable of degrading >90% of the toxin. The aflatoxin detoxifying activity of the T. ammi extract was drastically reduced upon boiling at 100 °C for 10 min. Significant levels of degradation of other aflatoxins viz., AFB1 (61%), AFB2 (54%) and AFG2 (46%) by the dialyzed T. ammi extract was also observed. Time course study of AFG1 detoxification by dialyzed T. ammi extract showed that more than 78% degradation occurred within 6 h and 91% degradation occurred 24 h after incubation. Mass spectral analysis of the degradation products of AFG1 revealed that the most abundant molecular ion peak in positive-ion mode was at m/z 288.29. MS/MS analysis of precursor ion at m/z 288.29 showed fragment transition to m/z 270.16 corresponding to the loss of 18 D. Mass spectral analysis of the degradation products of AFG1 suggests the modification of lactone ring structure. AFG1 caused more than 2% chromosomal aberration in corn at 40 mg/l concentration. However, AFG1 after treatment with T. ammi extract failed to induce chromosomal aberration demonstrating the degradation of AFG1 by T. ammi extract. The T. ammi extract may provide a biologically safe method to protect poultry or livestock feeds and other agricultural commodities from aflatoxins.

Original languageEnglish
Pages (from-to)719-725
Number of pages7
JournalFood Control
Volume21
Issue number5
DOIs
Publication statusPublished - May 2010

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Ammi
Trachyspermum ammi
aflatoxin G1
Aflatoxins
seed extracts
Medicinal Plants
aflatoxins
medicinal plants
Seeds
toxicity
degradation
extracts
Ions
chromosome aberrations
Chromosome Aberrations
ions
spectral analysis
Carum
Cotyledon
livestock feeds

Keywords

  • Aflatoxin
  • Detoxification
  • Mass spectral analysis
  • Trachyspermum ammi

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill - Structural analysis and biological toxicity of degradation product of aflatoxin G1. / Velazhahan, Rethinasamy; Vijayanandraj, Selvaraj; Vijayasamundeeswari, Ayyathurai; Paranidharan, Vaikuntavasen; Samiyappan, Ramasamy; Iwamoto, Takeo; Friebe, Bernd; Muthukrishnan, Subbaratnam.

In: Food Control, Vol. 21, No. 5, 05.2010, p. 719-725.

Research output: Contribution to journalArticle

Velazhahan, Rethinasamy ; Vijayanandraj, Selvaraj ; Vijayasamundeeswari, Ayyathurai ; Paranidharan, Vaikuntavasen ; Samiyappan, Ramasamy ; Iwamoto, Takeo ; Friebe, Bernd ; Muthukrishnan, Subbaratnam. / Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill - Structural analysis and biological toxicity of degradation product of aflatoxin G1. In: Food Control. 2010 ; Vol. 21, No. 5. pp. 719-725.
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abstract = "Aqueous extracts obtained from leaves/seeds of various medicinal plants were evaluated for their ability to detoxify aflatoxin G1 (AFG1) by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Of the various plant extracts, the seed extract of Ajowan (Trachyspermum ammi (L.) Sprague ex Turrill) showed the maximum degradation of AFG1 up to 65{\%}. The dialyzed T. ammi extract was more effective than the crude extract, capable of degrading >90{\%} of the toxin. The aflatoxin detoxifying activity of the T. ammi extract was drastically reduced upon boiling at 100 °C for 10 min. Significant levels of degradation of other aflatoxins viz., AFB1 (61{\%}), AFB2 (54{\%}) and AFG2 (46{\%}) by the dialyzed T. ammi extract was also observed. Time course study of AFG1 detoxification by dialyzed T. ammi extract showed that more than 78{\%} degradation occurred within 6 h and 91{\%} degradation occurred 24 h after incubation. Mass spectral analysis of the degradation products of AFG1 revealed that the most abundant molecular ion peak in positive-ion mode was at m/z 288.29. MS/MS analysis of precursor ion at m/z 288.29 showed fragment transition to m/z 270.16 corresponding to the loss of 18 D. Mass spectral analysis of the degradation products of AFG1 suggests the modification of lactone ring structure. AFG1 caused more than 2{\%} chromosomal aberration in corn at 40 mg/l concentration. However, AFG1 after treatment with T. ammi extract failed to induce chromosomal aberration demonstrating the degradation of AFG1 by T. ammi extract. The T. ammi extract may provide a biologically safe method to protect poultry or livestock feeds and other agricultural commodities from aflatoxins.",
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