Detection of genetically modified maize in Jordan

Abeer Aburumman, Hussein Migdadi, Muhanad Akash, Ayed Al-Abdallat, Yaser Hassan Dewir, Muhammad Farooq*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to detect genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. We used promoter 35 S and T-nos terminator for general screening of transgenic materials. Conventional PCR detected the specific events for the screening of Bt 11, MON810, and Bt176 events. Seeds of eleven maize hybrids samples showed a positive response to the 35 S promoter; nine out of eleven showed a positive response for T-nos terminator. Bt11 event was the most used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed the presence of the Bt176 event; however, MON810 not detected in any of the tested hybrids. We studied the Bt11 event in imported GMM seeds in Jordan for the first time, reinforcing the need for a mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalGM Crops and Food
Volume11
Issue number3
DOIs
Publication statusPublished - Jul 2 2020

Keywords

  • certified reference material
  • Genetically modified maize
  • labeling system
  • polymerase chain reaction

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science

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