Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom

A. D. Al-Zadjali, A. M. Al-Sadi, M. L. Deadman, S. Okuda, T. Natsuki, T. S. Al-Zadjali

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Beach naupaka (Scaevola taccada) showing witches' broom symptoms typical of phytoplasma infection were collected from a farm near Muscat (Sultanate of Oman). Polymerase chain reaction (PCR) amplification of the 16S rRNA gene and 16-23S intergenic spacer region with three pairs of phytoplasma universal primers (R16F2n/R2, AdF1/R1 and P1/P7) and transmission electron microscopy of phloem ultrathin sections, confirmed a phytoplasma infection in the symptomatic plants. RFLP analysis of the 16S rDNA sequences of the Scaevola witches' broom (ScaWB) and other phyto-plasmas reported from Oman using six restriction en-donucleases revealed that ScaWB, alfalfa witches' broom (AlfWB) and Jasmine witches' broom (JasWB) phytoplasmas shared similar RFLP patterns but different from those of the Lime witches' broom (LWB) phy-toplasma. Phylogenetic and sequence identity analyses of the 16S rRNA gene indicated that the ScaWB phyto-plasma belongs to the 16SrII group ('Candidatus Phyto-plasma aurantifolia', former Peanut witches' broom). The ScaWB phytoplasma showed 100% sequence identity with that of the Papaya yellow crinkle (PYC) and Tomato big bud (TBB) phytoplasmas. This is the first report of a phytoplasma related to 16SrII group associated with a witches' broom disease in S. taccada.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalJournal of Plant Pathology
Volume94
Issue number2
Publication statusPublished - 2012

Fingerprint

Scaevola taccada
witches' broom
Phytoplasma
Scaevola
16SrII (Peanut WB group)
phytoplasmal diseases
Oman
Tomato big bud phytoplasma
restriction fragment length polymorphism
ribosomal RNA
papayas
intergenic DNA
phloem
signs and symptoms (plants)
peanuts
transmission electron microscopy

Keywords

  • Diagnosis
  • PCR
  • Phytoplasma
  • RFLP
  • Scaevola taccada
  • SrII

ASJC Scopus subject areas

  • Plant Science

Cite this

Al-Zadjali, A. D., Al-Sadi, A. M., Deadman, M. L., Okuda, S., Natsuki, T., & Al-Zadjali, T. S. (2012). Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom. Journal of Plant Pathology, 94(2), 379-385.

Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom. / Al-Zadjali, A. D.; Al-Sadi, A. M.; Deadman, M. L.; Okuda, S.; Natsuki, T.; Al-Zadjali, T. S.

In: Journal of Plant Pathology, Vol. 94, No. 2, 2012, p. 379-385.

Research output: Contribution to journalArticle

Al-Zadjali, AD, Al-Sadi, AM, Deadman, ML, Okuda, S, Natsuki, T & Al-Zadjali, TS 2012, 'Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom', Journal of Plant Pathology, vol. 94, no. 2, pp. 379-385.
Al-Zadjali, A. D. ; Al-Sadi, A. M. ; Deadman, M. L. ; Okuda, S. ; Natsuki, T. ; Al-Zadjali, T. S. / Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom. In: Journal of Plant Pathology. 2012 ; Vol. 94, No. 2. pp. 379-385.
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