Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom

A. D. Al-Zadjali, A. M. Al-Sadi, M. L. Deadman, S. Okuda, T. Natsuki, T. S. Al-Zadjali

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Beach naupaka (Scaevola taccada) showing witches' broom symptoms typical of phytoplasma infection were collected from a farm near Muscat (Sultanate of Oman). Polymerase chain reaction (PCR) amplification of the 16S rRNA gene and 16-23S intergenic spacer region with three pairs of phytoplasma universal primers (R16F2n/R2, AdF1/R1 and P1/P7) and transmission electron microscopy of phloem ultrathin sections, confirmed a phytoplasma infection in the symptomatic plants. RFLP analysis of the 16S rDNA sequences of the Scaevola witches' broom (ScaWB) and other phyto-plasmas reported from Oman using six restriction en-donucleases revealed that ScaWB, alfalfa witches' broom (AlfWB) and Jasmine witches' broom (JasWB) phytoplasmas shared similar RFLP patterns but different from those of the Lime witches' broom (LWB) phy-toplasma. Phylogenetic and sequence identity analyses of the 16S rRNA gene indicated that the ScaWB phyto-plasma belongs to the 16SrII group ('Candidatus Phyto-plasma aurantifolia', former Peanut witches' broom). The ScaWB phytoplasma showed 100% sequence identity with that of the Papaya yellow crinkle (PYC) and Tomato big bud (TBB) phytoplasmas. This is the first report of a phytoplasma related to 16SrII group associated with a witches' broom disease in S. taccada.

Original languageEnglish
Pages (from-to)379-385
Number of pages7
JournalJournal of Plant Pathology
Volume94
Issue number2
Publication statusPublished - 2012

Keywords

  • Diagnosis
  • PCR
  • Phytoplasma
  • RFLP
  • Scaevola taccada
  • SrII

ASJC Scopus subject areas

  • Plant Science

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    Al-Zadjali, A. D., Al-Sadi, A. M., Deadman, M. L., Okuda, S., Natsuki, T., & Al-Zadjali, T. S. (2012). Detection, identification and molecular characterization of a phytoplasma associated with beach naupaka witches' broom. Journal of Plant Pathology, 94(2), 379-385.