Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens

Kenji Tani, William J. Murphy, Oleg Chertov, Rosalba Salcedo, Crystal Y. Koh, Iku Utsunomiya, Satoshi Funakoshi, Osamu Asai, Stephen H. Herrmann, Ji Ming Wang, Larry W. Kwak, Joost J. Oppenheim

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Defensins released by neutrophils are able to kill a broad spectrum of microbes. They also induce leukocyte migration in vitro and elicit inflammatory leukocyte responses at s.c. injection sites in mice. In vitro experiments showed that human defensins enhanced concanavalin A-stimulated murine spleen cell proliferation and IFN-γ production. This led us to examine the effects of human defensins on specific immune responses in vivo. BALB/c mice were immunized with 50 μg of keyhole limpet hemocyanin (KLH) adsorbed to aluminum hydroxide and administered with defensins in aqueous solution. Intraperitoneal administration of defensins significantly increased the production of KLH-specific IgG1, IgG2a and IgG2b antibodies 14 days after immunization. In vitro splenic KLH-specific proliferative responses were higher in mice treated with KLH and defensins than in those treated with KLH alone. Increased IFN-γ and, to a lesser extent, IL-4 production were also detected in the supernatants of ex vivo KLH-activated spleen cells from mice treated with defensins. Finally, defensins significantly enhanced the antibody response to a syngeneic tumor antigen, lymphoma Ig idiotype and also augmented resistance to tumor challenge. These results indicate that defensins act as potent immune adjuvants by inducing the production of lymphokines, which promote T cell-dependent cellular immunity and antigen-specific Ig production. Thus, defensins appear to function as neutrophil-derived signals that promote adaptive immune responses.

Original languageEnglish
Pages (from-to)691-700
Number of pages10
JournalInternational Immunology
Volume12
Issue number5
Publication statusPublished - 2000

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Defensins
Humoral Immunity
Cellular Immunity
Lymphoma
Antigens
Neutrophils
Leukocytes
Spleen
Aluminum Hydroxide
Lymphokines
Adaptive Immunity
Neoplasm Antigens
Concanavalin A
Interleukin-4
Antibody Formation
keyhole-limpet hemocyanin
Immunization
Immunoglobulin G
Cell Proliferation

Keywords

  • Antibody formation
  • Cytokine
  • IFN-γ
  • IL-4
  • Tumor antigen

ASJC Scopus subject areas

  • Immunology

Cite this

Tani, K., Murphy, W. J., Chertov, O., Salcedo, R., Koh, C. Y., Utsunomiya, I., ... Oppenheim, J. J. (2000). Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens. International Immunology, 12(5), 691-700.

Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens. / Tani, Kenji; Murphy, William J.; Chertov, Oleg; Salcedo, Rosalba; Koh, Crystal Y.; Utsunomiya, Iku; Funakoshi, Satoshi; Asai, Osamu; Herrmann, Stephen H.; Wang, Ji Ming; Kwak, Larry W.; Oppenheim, Joost J.

In: International Immunology, Vol. 12, No. 5, 2000, p. 691-700.

Research output: Contribution to journalArticle

Tani, K, Murphy, WJ, Chertov, O, Salcedo, R, Koh, CY, Utsunomiya, I, Funakoshi, S, Asai, O, Herrmann, SH, Wang, JM, Kwak, LW & Oppenheim, JJ 2000, 'Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens', International Immunology, vol. 12, no. 5, pp. 691-700.
Tani, Kenji ; Murphy, William J. ; Chertov, Oleg ; Salcedo, Rosalba ; Koh, Crystal Y. ; Utsunomiya, Iku ; Funakoshi, Satoshi ; Asai, Osamu ; Herrmann, Stephen H. ; Wang, Ji Ming ; Kwak, Larry W. ; Oppenheim, Joost J. / Defensins act as potent adjuvants that promote cellular and humoral immune responses in mice to a lymphoma idiotype and carrier antigens. In: International Immunology. 2000 ; Vol. 12, No. 5. pp. 691-700.
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