Defense responses in tomato landrace and wild genotypes to early blight pathogen Alternaria solani infection and accumulation of pathogenesisrelated proteins

Abida Puthenpeedikal Salim, Krishnaveni Saminaidu, Murugan Marimuthu, Yasodha Perumal, Velazhahan Rethinasamy, Jansi Rani Palanisami, Krishnasamy Vadivel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Plants dispense localised and systemic defense responses against biotic colonizers and plant resistance to pathogens depends upon timely recognition of pathogen infection and subsequent rapid activation of defense responses through signal transduction pathways. Induction of host defense responses involving compatible and incompatible interactions in tomato landrace, LE996, LE150 and LE1165 (Solanum lycopersicum), and wild relatives, Seijima Jeisei and I979 (S. hirsutum) genotypes, and early blight (EB) pathogen, Alternaria solani were studied. Accumulation patterns of different defense related proteins in resistant genotypes (LE996, Seijima jeisei and I979) and susceptible genotypes (LE150 and LE1165) are reported here. Challenge inoculation led to similar protein profiles in resistant genotypes yielding 11 proteins, unique are 14, 54, and 58 kDproteins that are absent with susceptible genotypes. Resistant genotypes accumulated more proteins well in advance, 6-h after challenge inoculation. Resistant genotype LE996 expressed 54%, 90%, and 52% enhanced enzymatic activity of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase respectively than susceptible LE150 and correlated phenolics accumulation peaked 2-d after challenge inoculation. Activity gel assay indicated the unique expression of PO1 and PO2 in LE996 upon challenge inoculation. Significant increase in expression of chitinases (63%) and β-1,3- glucanase (71%) upon challenge inoculation than susceptible LE150 control was recorded. Western blotting indicated the unique presence of less than 30 Kd chitinase in resistant LE996 and Seijima Jeisei and was absent in susceptible LE150. The results demonstrate the importance of pathogenesis-related (PR) proteins inEB resistance and their use as biochemical markers for genotype selection.

Original languageEnglish
Pages (from-to)1147-1164
Number of pages18
JournalArchives of Phytopathology and Plant Protection
Volume44
Issue number12
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Alternaria solani
blight
landraces
tomatoes
pathogens
genotype
infection
proteins
chitinase
pathogenesis-related proteins
wild relatives
Solanum lycopersicum
phenylalanine ammonia-lyase
catechol oxidase
signal transduction
biomarkers
peroxidase
Western blotting
gels
assays

Keywords

  • Early blight
  • PR proteins
  • Resistance
  • Tomato

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Defense responses in tomato landrace and wild genotypes to early blight pathogen Alternaria solani infection and accumulation of pathogenesisrelated proteins. / Salim, Abida Puthenpeedikal; Saminaidu, Krishnaveni; Marimuthu, Murugan; Perumal, Yasodha; Rethinasamy, Velazhahan; Palanisami, Jansi Rani; Vadivel, Krishnasamy.

In: Archives of Phytopathology and Plant Protection, Vol. 44, No. 12, 07.2011, p. 1147-1164.

Research output: Contribution to journalArticle

Salim, Abida Puthenpeedikal ; Saminaidu, Krishnaveni ; Marimuthu, Murugan ; Perumal, Yasodha ; Rethinasamy, Velazhahan ; Palanisami, Jansi Rani ; Vadivel, Krishnasamy. / Defense responses in tomato landrace and wild genotypes to early blight pathogen Alternaria solani infection and accumulation of pathogenesisrelated proteins. In: Archives of Phytopathology and Plant Protection. 2011 ; Vol. 44, No. 12. pp. 1147-1164.
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AU - Perumal, Yasodha

AU - Rethinasamy, Velazhahan

AU - Palanisami, Jansi Rani

AU - Vadivel, Krishnasamy

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AB - Plants dispense localised and systemic defense responses against biotic colonizers and plant resistance to pathogens depends upon timely recognition of pathogen infection and subsequent rapid activation of defense responses through signal transduction pathways. Induction of host defense responses involving compatible and incompatible interactions in tomato landrace, LE996, LE150 and LE1165 (Solanum lycopersicum), and wild relatives, Seijima Jeisei and I979 (S. hirsutum) genotypes, and early blight (EB) pathogen, Alternaria solani were studied. Accumulation patterns of different defense related proteins in resistant genotypes (LE996, Seijima jeisei and I979) and susceptible genotypes (LE150 and LE1165) are reported here. Challenge inoculation led to similar protein profiles in resistant genotypes yielding 11 proteins, unique are 14, 54, and 58 kDproteins that are absent with susceptible genotypes. Resistant genotypes accumulated more proteins well in advance, 6-h after challenge inoculation. Resistant genotype LE996 expressed 54%, 90%, and 52% enhanced enzymatic activity of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase respectively than susceptible LE150 and correlated phenolics accumulation peaked 2-d after challenge inoculation. Activity gel assay indicated the unique expression of PO1 and PO2 in LE996 upon challenge inoculation. Significant increase in expression of chitinases (63%) and β-1,3- glucanase (71%) upon challenge inoculation than susceptible LE150 control was recorded. Western blotting indicated the unique presence of less than 30 Kd chitinase in resistant LE996 and Seijima Jeisei and was absent in susceptible LE150. The results demonstrate the importance of pathogenesis-related (PR) proteins inEB resistance and their use as biochemical markers for genotype selection.

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