De novo phosphoinositide synthesis in zebrafish is required for triad formation but not essential for myogenesis

Lindsay Smith, Lacramioara Fabian, Almundher Al-Maawali, Ramil R. Noche, James J. Dowling*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphoinositides (PIPs) and their regulatory enzymes are key players in many cellular processes and are required for aspects of vertebrate development. Dysregulated PIP metabolism has been implicated in several human diseases, including a subset of skeletal myopathies that feature structural defects in the triad. The role of PIPs in skeletal muscle formation, and particularly triad biogenesis, has yet to be determined. CDP-diacylglycerol-inositol 3-phosphatidyltransferase (CDIPT) catalyzes the formation of phosphatidylinositol, which is the base of all PIP species. Loss of CDIPT should, in theory, result in the failure to produce PIPs, and thus provide a strategy for establishing the requirement for PIPs during embryogenesis. In this study, we generated cdipt mutant zebrafish and determined the impact on skeletal myogenesis. Analysis of cdipt mutant muscle revealed no apparent global effect on early muscle development. However, small but significant defects were observed in triad size, with T-tubule area, inter terminal cisternae distance and gap width being smaller in cdipt mutants. This was associated with a decrease in motor performance. Overall, these data suggest that myogenesis in zebrafish does not require de novo PIP synthesis but does implicate a role for CDIPT in triad formation.

Original languageEnglish
Article numbere0231364
JournalPLoS One
Volume15
Issue number8 August
DOIs
Publication statusPublished - Aug 2020
Externally publishedYes

ASJC Scopus subject areas

  • General

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