Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii

J. Michael Conlon, Jolanta Kolodziejek, Norbert Nowotny, Jérôme Leprince, Hubert Vaudry, Laurent Coquet, Thierry Jouenne, Shawichi Iwamuro

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Peptidomic analysis of an extract of the skins of specimens of Dybowski's brown frog Rana dybowskii Gunther, 1876, collected on Tsushima Island, Japan led to the identification of 10 peptides with differential antibacterial and hemolytic activities. The primary structures of these peptides identified them as belonging to the brevinin-1 (5 peptides) and brevinin-2 (5 peptides) families of antimicrobial peptides. A peptide (FIGPIISALASLFG.NH2) with structural similarity to members of the temporin family was also isolated but this component lacked cytolytic activity. Phylogenetic relationships among the Japanese brown frogs (R. dybowskii, R. japonica, R. okinavana, R. ornativentris, R. pirica, R. sakuraii, R. tagoi, and R. tsushimensis) are only incompletely understood. Cladograms based upon maximum parsimony analyses of the brevinin-1 and brevinin-2 amino acid sequences provide strong support for a sister-group relationship between R. dybowskii and R. pirica and somewhat weaker support for a sister-group relationship between R. okinavana and R. tsushimensis. These conclusions are consistent with previous analyses based upon allozyme variations and comparisons of the nucleotide sequences of mitochondrial genes.

Original languageEnglish
Pages (from-to)746-756
Number of pages11
JournalToxicon
Volume50
Issue number6
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Ranidae
Anura
Skin
Peptides
Siblings
Mitochondrial Genes
Islands
Isoenzymes
Amino Acid Sequence
Japan
Nucleotides
Genes
Amino Acids

Keywords

  • Antimicrobial peptide
  • Brevinin-1
  • Brevinin-2
  • Frog phylogeny
  • Temporin

ASJC Scopus subject areas

  • Toxicology

Cite this

Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii. / Conlon, J. Michael; Kolodziejek, Jolanta; Nowotny, Norbert; Leprince, Jérôme; Vaudry, Hubert; Coquet, Laurent; Jouenne, Thierry; Iwamuro, Shawichi.

In: Toxicon, Vol. 50, No. 6, 11.2007, p. 746-756.

Research output: Contribution to journalArticle

Conlon, J. Michael ; Kolodziejek, Jolanta ; Nowotny, Norbert ; Leprince, Jérôme ; Vaudry, Hubert ; Coquet, Laurent ; Jouenne, Thierry ; Iwamuro, Shawichi. / Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii. In: Toxicon. 2007 ; Vol. 50, No. 6. pp. 746-756.
@article{d57d2e1a4b9445ee8fbaa421a4f1ecd6,
title = "Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii",
abstract = "Peptidomic analysis of an extract of the skins of specimens of Dybowski's brown frog Rana dybowskii Gunther, 1876, collected on Tsushima Island, Japan led to the identification of 10 peptides with differential antibacterial and hemolytic activities. The primary structures of these peptides identified them as belonging to the brevinin-1 (5 peptides) and brevinin-2 (5 peptides) families of antimicrobial peptides. A peptide (FIGPIISALASLFG.NH2) with structural similarity to members of the temporin family was also isolated but this component lacked cytolytic activity. Phylogenetic relationships among the Japanese brown frogs (R. dybowskii, R. japonica, R. okinavana, R. ornativentris, R. pirica, R. sakuraii, R. tagoi, and R. tsushimensis) are only incompletely understood. Cladograms based upon maximum parsimony analyses of the brevinin-1 and brevinin-2 amino acid sequences provide strong support for a sister-group relationship between R. dybowskii and R. pirica and somewhat weaker support for a sister-group relationship between R. okinavana and R. tsushimensis. These conclusions are consistent with previous analyses based upon allozyme variations and comparisons of the nucleotide sequences of mitochondrial genes.",
keywords = "Antimicrobial peptide, Brevinin-1, Brevinin-2, Frog phylogeny, Temporin",
author = "Conlon, {J. Michael} and Jolanta Kolodziejek and Norbert Nowotny and J{\'e}r{\^o}me Leprince and Hubert Vaudry and Laurent Coquet and Thierry Jouenne and Shawichi Iwamuro",
year = "2007",
month = "11",
doi = "10.1016/j.toxicon.2007.06.023",
language = "English",
volume = "50",
pages = "746--756",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii

AU - Conlon, J. Michael

AU - Kolodziejek, Jolanta

AU - Nowotny, Norbert

AU - Leprince, Jérôme

AU - Vaudry, Hubert

AU - Coquet, Laurent

AU - Jouenne, Thierry

AU - Iwamuro, Shawichi

PY - 2007/11

Y1 - 2007/11

N2 - Peptidomic analysis of an extract of the skins of specimens of Dybowski's brown frog Rana dybowskii Gunther, 1876, collected on Tsushima Island, Japan led to the identification of 10 peptides with differential antibacterial and hemolytic activities. The primary structures of these peptides identified them as belonging to the brevinin-1 (5 peptides) and brevinin-2 (5 peptides) families of antimicrobial peptides. A peptide (FIGPIISALASLFG.NH2) with structural similarity to members of the temporin family was also isolated but this component lacked cytolytic activity. Phylogenetic relationships among the Japanese brown frogs (R. dybowskii, R. japonica, R. okinavana, R. ornativentris, R. pirica, R. sakuraii, R. tagoi, and R. tsushimensis) are only incompletely understood. Cladograms based upon maximum parsimony analyses of the brevinin-1 and brevinin-2 amino acid sequences provide strong support for a sister-group relationship between R. dybowskii and R. pirica and somewhat weaker support for a sister-group relationship between R. okinavana and R. tsushimensis. These conclusions are consistent with previous analyses based upon allozyme variations and comparisons of the nucleotide sequences of mitochondrial genes.

AB - Peptidomic analysis of an extract of the skins of specimens of Dybowski's brown frog Rana dybowskii Gunther, 1876, collected on Tsushima Island, Japan led to the identification of 10 peptides with differential antibacterial and hemolytic activities. The primary structures of these peptides identified them as belonging to the brevinin-1 (5 peptides) and brevinin-2 (5 peptides) families of antimicrobial peptides. A peptide (FIGPIISALASLFG.NH2) with structural similarity to members of the temporin family was also isolated but this component lacked cytolytic activity. Phylogenetic relationships among the Japanese brown frogs (R. dybowskii, R. japonica, R. okinavana, R. ornativentris, R. pirica, R. sakuraii, R. tagoi, and R. tsushimensis) are only incompletely understood. Cladograms based upon maximum parsimony analyses of the brevinin-1 and brevinin-2 amino acid sequences provide strong support for a sister-group relationship between R. dybowskii and R. pirica and somewhat weaker support for a sister-group relationship between R. okinavana and R. tsushimensis. These conclusions are consistent with previous analyses based upon allozyme variations and comparisons of the nucleotide sequences of mitochondrial genes.

KW - Antimicrobial peptide

KW - Brevinin-1

KW - Brevinin-2

KW - Frog phylogeny

KW - Temporin

UR - http://www.scopus.com/inward/record.url?scp=34548827045&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548827045&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2007.06.023

DO - 10.1016/j.toxicon.2007.06.023

M3 - Article

VL - 50

SP - 746

EP - 756

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 6

ER -