Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001: Comparison with the South African Strain SAAR-1776 and other flaviviruses

Tamás Bakonyi, Ernest A. Gould, Jolanta Kolodziejek, Herbert Weissenböck, Norbert Nowotny

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Here we describe the complete genome sequences of two strains of Usutu virus (USUV), a mosquito-borne member of the genus Flavivirus in the Japanese encephalitis virus (JEV) serogroup. USUV was detected in Austria in 2001 causing a high mortality rate in blackbirds; the reference strain (SAAR-1776) was isolated in 1958 from mosquitoes in South Africa and has never been associated with avian mortality. The Austrian and South African isolates exhibited 97% nucleotide and 99% amino acid identity. Phylogenetic trees were constructed displaying the genetic relationships of USUV with other members of the genus Flavivirus. When comparing USUV with other JEV serogroup viruses, the closest lineage was Murray Valley encephalitis virus (nt: 73%, aa: 82%) followed by JEV (nt: 71%, aa: 81%) and West Nile virus (nt: 68%, aa: 75%). Comparison of the genomes showed that the conserved structural elements and putative enzyme motifs were homologous in the two USUV strains and the JEV serogroup. The factors that determine the severe clinical symptoms caused by the Austrian USUV strain in Eurasian blackbirds are discussed. We also offer a possible explanation for the origins and dispersal of USUV, JEV, and MVEV out of Africa.

Original languageEnglish
Pages (from-to)301-310
Number of pages10
JournalVirology
Volume328
Issue number2
DOIs
Publication statusPublished - Oct 25 2004

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Flavivirus
Austria
Japanese Encephalitis Virus
Genome
Viruses
Culicidae
Murray Valley encephalitis virus
West Nile virus
Mortality
South Africa
Nucleotides
Amino Acids
Enzymes

Keywords

  • Austria
  • Blackbird mortality
  • Complete nucleotide sequences
  • Emerging mosquito-borne flavivirus
  • Flaviviridae
  • Genome analysis and comparison
  • Phylogenetic analysis
  • SAAR-1776
  • Usutu virus
  • USUV

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001 : Comparison with the South African Strain SAAR-1776 and other flaviviruses. / Bakonyi, Tamás; Gould, Ernest A.; Kolodziejek, Jolanta; Weissenböck, Herbert; Nowotny, Norbert.

In: Virology, Vol. 328, No. 2, 25.10.2004, p. 301-310.

Research output: Contribution to journalArticle

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