Cold-active winter rye glucanases with ice-binding capacity

M. W F Yaish, Andrew C. Doxey, Brendan J. McConkey, Barbara A. Moffatt, Marilyn Griffith

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Extracellular pathogenesis-related proteins, including glucanases, are expressed at cold temperatures in winter rye (Secale cereale) and display antifreeze activity. We have characterized recombinant cold-induced glucanases from winter rye to further examine their roles and contributions to cold tolerance. Both basic β-1,3-glucanases and an acidic β-1,3;1,4- glucanase were expressed in Escherichia coli, purified, and assayed for their hydrolytic and antifreeze activities in vitro. All were found to be cold active and to retain partial hydrolytic activity at subzero temperatures (e.g. 14%-35% at -4°C). The two types of glucanases had antifreeze activity as measured by their ability to modify the growth of ice crystals. Structural models for the winter rye β-1,3-glucanases were developed on which putative ice-binding surfaces (IBSs) were identified. Residues on the putative IBSs were charge conserved for each of the expressed glucanases, with the exception of one β-1,3-glucanase recovered from nonacclimated winter rye in which a charged amino acid was present on the putative IBS. This protein also had a reduced antifreeze activity relative to the other expressed glucanases. These results support the hypothesis that winter rye glucanases have evolved to inhibit the formation of large, potentially fatal ice crystals, in addition to having enzymatic activity with a potential role in resisting infection by psychrophilic pathogens. Glucanases of winter rye provide an interesting example of protein evolution and adaptation aimed to combat cold and freezing conditions.

Original languageEnglish
Pages (from-to)1459-1472
Number of pages14
JournalPlant Physiology
Volume141
Issue number4
DOIs
Publication statusPublished - Aug 2006

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Ice
binding capacity
rye
ice
winter
crystals
pathogenesis-related proteins
Secale cereale
Proteins
cold tolerance
Structural Models
Crystallization
Secale
freezing
temperature
Freezing
proteins
Escherichia coli
amino acids
pathogens

ASJC Scopus subject areas

  • Plant Science

Cite this

Yaish, M. W. F., Doxey, A. C., McConkey, B. J., Moffatt, B. A., & Griffith, M. (2006). Cold-active winter rye glucanases with ice-binding capacity. Plant Physiology, 141(4), 1459-1472. https://doi.org/10.1104/pp.106.081935

Cold-active winter rye glucanases with ice-binding capacity. / Yaish, M. W F; Doxey, Andrew C.; McConkey, Brendan J.; Moffatt, Barbara A.; Griffith, Marilyn.

In: Plant Physiology, Vol. 141, No. 4, 08.2006, p. 1459-1472.

Research output: Contribution to journalArticle

Yaish, MWF, Doxey, AC, McConkey, BJ, Moffatt, BA & Griffith, M 2006, 'Cold-active winter rye glucanases with ice-binding capacity', Plant Physiology, vol. 141, no. 4, pp. 1459-1472. https://doi.org/10.1104/pp.106.081935
Yaish, M. W F ; Doxey, Andrew C. ; McConkey, Brendan J. ; Moffatt, Barbara A. ; Griffith, Marilyn. / Cold-active winter rye glucanases with ice-binding capacity. In: Plant Physiology. 2006 ; Vol. 141, No. 4. pp. 1459-1472.
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