Cold-active winter rye glucanases with ice-binding capacity

M. W F Yaish*, Andrew C. Doxey, Brendan J. McConkey, Barbara A. Moffatt, Marilyn Griffith

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Extracellular pathogenesis-related proteins, including glucanases, are expressed at cold temperatures in winter rye (Secale cereale) and display antifreeze activity. We have characterized recombinant cold-induced glucanases from winter rye to further examine their roles and contributions to cold tolerance. Both basic β-1,3-glucanases and an acidic β-1,3;1,4- glucanase were expressed in Escherichia coli, purified, and assayed for their hydrolytic and antifreeze activities in vitro. All were found to be cold active and to retain partial hydrolytic activity at subzero temperatures (e.g. 14%-35% at -4°C). The two types of glucanases had antifreeze activity as measured by their ability to modify the growth of ice crystals. Structural models for the winter rye β-1,3-glucanases were developed on which putative ice-binding surfaces (IBSs) were identified. Residues on the putative IBSs were charge conserved for each of the expressed glucanases, with the exception of one β-1,3-glucanase recovered from nonacclimated winter rye in which a charged amino acid was present on the putative IBS. This protein also had a reduced antifreeze activity relative to the other expressed glucanases. These results support the hypothesis that winter rye glucanases have evolved to inhibit the formation of large, potentially fatal ice crystals, in addition to having enzymatic activity with a potential role in resisting infection by psychrophilic pathogens. Glucanases of winter rye provide an interesting example of protein evolution and adaptation aimed to combat cold and freezing conditions.

Original languageEnglish
Pages (from-to)1459-1472
Number of pages14
JournalPlant Physiology
Volume141
Issue number4
DOIs
Publication statusPublished - Aug 2006

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of 'Cold-active winter rye glucanases with ice-binding capacity'. Together they form a unique fingerprint.

  • Cite this

    Yaish, M. W. F., Doxey, A. C., McConkey, B. J., Moffatt, B. A., & Griffith, M. (2006). Cold-active winter rye glucanases with ice-binding capacity. Plant Physiology, 141(4), 1459-1472. https://doi.org/10.1104/pp.106.081935