Chemoprevention of rat mammary carcinogenesis by spirulina

Allal Ouhtit, Mohammed F. Ismail, Amira Othman, Augusta Fernando, Mohamed E. Abdraboh, Attalla F. El-Kott, Yahia A. Azab, Sherif H. Abdeen, Rajiv L. Gaur, Ishita Gupta, Somya Shanmuganathan, Yahya M. Al-Farsi, Hamad Al-Riyami, Madhwa H G Raj

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Spirulina (SP) (Arthrospira platensis; previously Spirulina platensis) is a filamentous blue-green microalga (cyanobacterium) with potent dietary phytoantioxidant and anticancerous properties. We investigated the chemopreventive effect of SP against 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat breast carcinogenesis, and further studied its underlying mechanisms of action in vitro. Remarkably, SP cleared DMBA-induced rat mammary tumors, which was clearly confirmed by morphological and histological methods. SP supplementation reduced the incidence of breast tumors from 87% to 13%. At the molecular level, immunohistochemical analysis revealed that SP supplementation reduced expression of both Ki-67 and estrogen α. More interestingly, molecular analysis in the in vitro experiments indicated that SP treatment inhibited cell proliferation by 24 hours, which was accompanied by increased p53 expression, followed by increased expression of its downstream target gene, Cdkn1a (alias p21 or p21Waf1/Cip1). In addition, SP increased Bax and decreased Bcl-2 expression, indicating induction of apoptosis by 48 hours after SP treatment. To our knowledge, this is the first report of in vivo chemopreventive effect of SP against DMBA-induced breast carcinogenesis in rat, supporting its potential use in chemoprevention of cancer.

Original languageEnglish
Pages (from-to)296-303
Number of pages8
JournalAmerican Journal of Pathology
Volume184
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

Spirulina
Chemoprevention
Carcinogenesis
Breast
9,10-Dimethyl-1,2-benzanthracene
Breast Neoplasms
Cyanobacteria
Estrogens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ouhtit, A., Ismail, M. F., Othman, A., Fernando, A., Abdraboh, M. E., El-Kott, A. F., ... Raj, M. H. G. (2014). Chemoprevention of rat mammary carcinogenesis by spirulina. American Journal of Pathology, 184(1), 296-303. https://doi.org/10.1016/j.ajpath.2013.10.025

Chemoprevention of rat mammary carcinogenesis by spirulina. / Ouhtit, Allal; Ismail, Mohammed F.; Othman, Amira; Fernando, Augusta; Abdraboh, Mohamed E.; El-Kott, Attalla F.; Azab, Yahia A.; Abdeen, Sherif H.; Gaur, Rajiv L.; Gupta, Ishita; Shanmuganathan, Somya; Al-Farsi, Yahya M.; Al-Riyami, Hamad; Raj, Madhwa H G.

In: American Journal of Pathology, Vol. 184, No. 1, 01.2014, p. 296-303.

Research output: Contribution to journalArticle

Ouhtit, A, Ismail, MF, Othman, A, Fernando, A, Abdraboh, ME, El-Kott, AF, Azab, YA, Abdeen, SH, Gaur, RL, Gupta, I, Shanmuganathan, S, Al-Farsi, YM, Al-Riyami, H & Raj, MHG 2014, 'Chemoprevention of rat mammary carcinogenesis by spirulina', American Journal of Pathology, vol. 184, no. 1, pp. 296-303. https://doi.org/10.1016/j.ajpath.2013.10.025
Ouhtit A, Ismail MF, Othman A, Fernando A, Abdraboh ME, El-Kott AF et al. Chemoprevention of rat mammary carcinogenesis by spirulina. American Journal of Pathology. 2014 Jan;184(1):296-303. https://doi.org/10.1016/j.ajpath.2013.10.025
Ouhtit, Allal ; Ismail, Mohammed F. ; Othman, Amira ; Fernando, Augusta ; Abdraboh, Mohamed E. ; El-Kott, Attalla F. ; Azab, Yahia A. ; Abdeen, Sherif H. ; Gaur, Rajiv L. ; Gupta, Ishita ; Shanmuganathan, Somya ; Al-Farsi, Yahya M. ; Al-Riyami, Hamad ; Raj, Madhwa H G. / Chemoprevention of rat mammary carcinogenesis by spirulina. In: American Journal of Pathology. 2014 ; Vol. 184, No. 1. pp. 296-303.
@article{8ca2799564da450ba19959039111cd1b,
title = "Chemoprevention of rat mammary carcinogenesis by spirulina",
abstract = "Spirulina (SP) (Arthrospira platensis; previously Spirulina platensis) is a filamentous blue-green microalga (cyanobacterium) with potent dietary phytoantioxidant and anticancerous properties. We investigated the chemopreventive effect of SP against 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat breast carcinogenesis, and further studied its underlying mechanisms of action in vitro. Remarkably, SP cleared DMBA-induced rat mammary tumors, which was clearly confirmed by morphological and histological methods. SP supplementation reduced the incidence of breast tumors from 87{\%} to 13{\%}. At the molecular level, immunohistochemical analysis revealed that SP supplementation reduced expression of both Ki-67 and estrogen α. More interestingly, molecular analysis in the in vitro experiments indicated that SP treatment inhibited cell proliferation by 24 hours, which was accompanied by increased p53 expression, followed by increased expression of its downstream target gene, Cdkn1a (alias p21 or p21Waf1/Cip1). In addition, SP increased Bax and decreased Bcl-2 expression, indicating induction of apoptosis by 48 hours after SP treatment. To our knowledge, this is the first report of in vivo chemopreventive effect of SP against DMBA-induced breast carcinogenesis in rat, supporting its potential use in chemoprevention of cancer.",
author = "Allal Ouhtit and Ismail, {Mohammed F.} and Amira Othman and Augusta Fernando and Abdraboh, {Mohamed E.} and El-Kott, {Attalla F.} and Azab, {Yahia A.} and Abdeen, {Sherif H.} and Gaur, {Rajiv L.} and Ishita Gupta and Somya Shanmuganathan and Al-Farsi, {Yahya M.} and Hamad Al-Riyami and Raj, {Madhwa H G}",
year = "2014",
month = "1",
doi = "10.1016/j.ajpath.2013.10.025",
language = "English",
volume = "184",
pages = "296--303",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Chemoprevention of rat mammary carcinogenesis by spirulina

AU - Ouhtit, Allal

AU - Ismail, Mohammed F.

AU - Othman, Amira

AU - Fernando, Augusta

AU - Abdraboh, Mohamed E.

AU - El-Kott, Attalla F.

AU - Azab, Yahia A.

AU - Abdeen, Sherif H.

AU - Gaur, Rajiv L.

AU - Gupta, Ishita

AU - Shanmuganathan, Somya

AU - Al-Farsi, Yahya M.

AU - Al-Riyami, Hamad

AU - Raj, Madhwa H G

PY - 2014/1

Y1 - 2014/1

N2 - Spirulina (SP) (Arthrospira platensis; previously Spirulina platensis) is a filamentous blue-green microalga (cyanobacterium) with potent dietary phytoantioxidant and anticancerous properties. We investigated the chemopreventive effect of SP against 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat breast carcinogenesis, and further studied its underlying mechanisms of action in vitro. Remarkably, SP cleared DMBA-induced rat mammary tumors, which was clearly confirmed by morphological and histological methods. SP supplementation reduced the incidence of breast tumors from 87% to 13%. At the molecular level, immunohistochemical analysis revealed that SP supplementation reduced expression of both Ki-67 and estrogen α. More interestingly, molecular analysis in the in vitro experiments indicated that SP treatment inhibited cell proliferation by 24 hours, which was accompanied by increased p53 expression, followed by increased expression of its downstream target gene, Cdkn1a (alias p21 or p21Waf1/Cip1). In addition, SP increased Bax and decreased Bcl-2 expression, indicating induction of apoptosis by 48 hours after SP treatment. To our knowledge, this is the first report of in vivo chemopreventive effect of SP against DMBA-induced breast carcinogenesis in rat, supporting its potential use in chemoprevention of cancer.

AB - Spirulina (SP) (Arthrospira platensis; previously Spirulina platensis) is a filamentous blue-green microalga (cyanobacterium) with potent dietary phytoantioxidant and anticancerous properties. We investigated the chemopreventive effect of SP against 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat breast carcinogenesis, and further studied its underlying mechanisms of action in vitro. Remarkably, SP cleared DMBA-induced rat mammary tumors, which was clearly confirmed by morphological and histological methods. SP supplementation reduced the incidence of breast tumors from 87% to 13%. At the molecular level, immunohistochemical analysis revealed that SP supplementation reduced expression of both Ki-67 and estrogen α. More interestingly, molecular analysis in the in vitro experiments indicated that SP treatment inhibited cell proliferation by 24 hours, which was accompanied by increased p53 expression, followed by increased expression of its downstream target gene, Cdkn1a (alias p21 or p21Waf1/Cip1). In addition, SP increased Bax and decreased Bcl-2 expression, indicating induction of apoptosis by 48 hours after SP treatment. To our knowledge, this is the first report of in vivo chemopreventive effect of SP against DMBA-induced breast carcinogenesis in rat, supporting its potential use in chemoprevention of cancer.

UR - http://www.scopus.com/inward/record.url?scp=84890340678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890340678&partnerID=8YFLogxK

U2 - 10.1016/j.ajpath.2013.10.025

DO - 10.1016/j.ajpath.2013.10.025

M3 - Article

VL - 184

SP - 296

EP - 303

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 1

ER -