Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina

Mohamed F. Ismail, Doaa A. Ali, Augusta Fernando, Mohamed E. Abdraboh, Rajiv L. Gaur, Wael M. Ibrahim, Madhwa H G Raj, Allal Ouhtit

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Spirulina platensis (SP) is a filamentous cyanobacterium microalgae with potent dietary phyto-antioxidant, anti-inflammatory and anti-cancerous properties. The present study aimed to investigate the chemopreventive effect of SP against rat liver toxicity and carcinogenesis induced by dibutyl nitrosamine (DBN) precursors, and further characterized its underlying mechanisms of action in HepG2 cell line. Investigation by light and electron microscopy showed that DBN treatment induced severe liver injury and histopathological abnormalities, which were prevented by SP supplementation. The incidence of liver tumors was significantly reduced from 80 to 20% by SP. Immunohistochemical results indicated that both PCNA and p53 were highly expressed in the liver of DBN-treated rats, but were significantly reduced by SP supplementation. Molecular analysis indicated that SP treatment inhibited cell proliferation, which was accompanied by increased p21 and decreased Rb expression levels at 48hrs post-treatment. In addition, SP increased Bax and decreased Bcl-2 expression, indicating induction of apoptosis by 48hrs. This is the first report of the in vivo chemopreventive effect of SP against DBN-induced rat liver cytotoxicity and carcinogenesis, suggesting its potential use in chemoprevention of cancer.

Original languageEnglish
Pages (from-to)377-387
Number of pages11
JournalInternational Journal of Biological Sciences
Volume5
Issue number4
Publication statusPublished - 2009

Fingerprint

Spirulina
Spirulina platensis
chemoprevention
hepatotoxicity
Chemoprevention
carcinogenesis
Carcinogenesis
toxicity
nitrosamines
Nitrosamines
Liver
rats
molecular analysis
apoptosis
abnormality
electron microscopy
tumor
antioxidant
liver
cyanobacterium

Keywords

  • Electron microscopy
  • Hepatocellular carcinoma
  • Liver toxicity
  • P53
  • Phyto-antioxidant
  • Spirulina platensis

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

Ismail, M. F., Ali, D. A., Fernando, A., Abdraboh, M. E., Gaur, R. L., Ibrahim, W. M., ... Ouhtit, A. (2009). Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina. International Journal of Biological Sciences, 5(4), 377-387.

Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina. / Ismail, Mohamed F.; Ali, Doaa A.; Fernando, Augusta; Abdraboh, Mohamed E.; Gaur, Rajiv L.; Ibrahim, Wael M.; Raj, Madhwa H G; Ouhtit, Allal.

In: International Journal of Biological Sciences, Vol. 5, No. 4, 2009, p. 377-387.

Research output: Contribution to journalArticle

Ismail, MF, Ali, DA, Fernando, A, Abdraboh, ME, Gaur, RL, Ibrahim, WM, Raj, MHG & Ouhtit, A 2009, 'Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina', International Journal of Biological Sciences, vol. 5, no. 4, pp. 377-387.
Ismail MF, Ali DA, Fernando A, Abdraboh ME, Gaur RL, Ibrahim WM et al. Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina. International Journal of Biological Sciences. 2009;5(4):377-387.
Ismail, Mohamed F. ; Ali, Doaa A. ; Fernando, Augusta ; Abdraboh, Mohamed E. ; Gaur, Rajiv L. ; Ibrahim, Wael M. ; Raj, Madhwa H G ; Ouhtit, Allal. / Chemoprevention of rat liver toxicity and carcinogenesis by Spirulina. In: International Journal of Biological Sciences. 2009 ; Vol. 5, No. 4. pp. 377-387.
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