Chemical composition and anti-proliferative effect of Oman's Ganoderma applanatum on breast cancer and cervical cancer cells

Faruck L. Hakkim, Mohammed Al-Buloshi, Jackson Achankunju

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives To evaluate the anti-proliferative potential of Oman's Ganoderma applanatum on triple negative breast cancer cells (MDA-MB-231), cervical cancer cells (HEp-2) and Vero cells (normal) and its chemical profiling of G. applanatum. Methods The cytotoxicity of the methanolic extract of G. applanatum was tested on MDA-MB-231 cells, HEp-2 cells and Vero cells by microculture tetrazolium (MTT) assay. The anti-cancer properties of G. applanatum were characterized using gas chromatography coupled with gas chromatography mass spectrometry (GCMS) analysis. Results The methanolic extract of G. applanatum elicited dose-dependent cell death on both MDA-MB-231 and HEp-2 cells after 24 h of treatment, with an IC50 value of 84.6 μg/ml and 43.2 μg/ml, respectively. Normal vero cells were able to significantly withstand the treatment. Furthermore, seven molecules were identified from G. applanatum by GCMS analysis, and five of them were present in considerable amounts, namely, γ-terpinene (30.3%), d-limonene (23.6%), cis-2-methyl-4-pentylthiane-s,s-dioxide (15.3%), β-cymene (12.7%) and α-terpinolene (8.1%). Conclusion In this study, surprisingly, G. applanatum exhibited toxicity particularly towards tumour cells (MDA-MB-231 and HEp-2) compared to normal vero cells. This tumour-specific chemo-sensitivity of G. applanatum is unclear, and the mechanism of cancer cell death induced by G. applanatum should be studied in detail. In addition, the observed anti-cancer activity of G. applanatum might be due to the synergistic effects of the identified properties. This would pave the way for isolation and characterization of the potential anti-cancer molecule from Oman's G. applanatum.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalJournal of Taibah University Medical Sciences
Volume11
Issue number2
DOIs
Publication statusPublished - Apr 1 2016

Fingerprint

Ganoderma
Oman
Uterine Cervical Neoplasms
Breast Neoplasms
Vero Cells
Neoplasms
Gas Chromatography-Mass Spectrometry
Cell Death
Triple Negative Breast Neoplasms
Gas Chromatography
Inhibitory Concentration 50

Keywords

  • Breast cancer
  • Cervical cancer
  • D-Limonene
  • Ganoderma applanatum
  • Oman
  • γ-Terpinene

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chemical composition and anti-proliferative effect of Oman's Ganoderma applanatum on breast cancer and cervical cancer cells. / Hakkim, Faruck L.; Al-Buloshi, Mohammed; Achankunju, Jackson.

In: Journal of Taibah University Medical Sciences, Vol. 11, No. 2, 01.04.2016, p. 145-151.

Research output: Contribution to journalArticle

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title = "Chemical composition and anti-proliferative effect of Oman's Ganoderma applanatum on breast cancer and cervical cancer cells",
abstract = "Objectives To evaluate the anti-proliferative potential of Oman's Ganoderma applanatum on triple negative breast cancer cells (MDA-MB-231), cervical cancer cells (HEp-2) and Vero cells (normal) and its chemical profiling of G. applanatum. Methods The cytotoxicity of the methanolic extract of G. applanatum was tested on MDA-MB-231 cells, HEp-2 cells and Vero cells by microculture tetrazolium (MTT) assay. The anti-cancer properties of G. applanatum were characterized using gas chromatography coupled with gas chromatography mass spectrometry (GCMS) analysis. Results The methanolic extract of G. applanatum elicited dose-dependent cell death on both MDA-MB-231 and HEp-2 cells after 24 h of treatment, with an IC50 value of 84.6 μg/ml and 43.2 μg/ml, respectively. Normal vero cells were able to significantly withstand the treatment. Furthermore, seven molecules were identified from G. applanatum by GCMS analysis, and five of them were present in considerable amounts, namely, γ-terpinene (30.3{\%}), d-limonene (23.6{\%}), cis-2-methyl-4-pentylthiane-s,s-dioxide (15.3{\%}), β-cymene (12.7{\%}) and α-terpinolene (8.1{\%}). Conclusion In this study, surprisingly, G. applanatum exhibited toxicity particularly towards tumour cells (MDA-MB-231 and HEp-2) compared to normal vero cells. This tumour-specific chemo-sensitivity of G. applanatum is unclear, and the mechanism of cancer cell death induced by G. applanatum should be studied in detail. In addition, the observed anti-cancer activity of G. applanatum might be due to the synergistic effects of the identified properties. This would pave the way for isolation and characterization of the potential anti-cancer molecule from Oman's G. applanatum.",
keywords = "Breast cancer, Cervical cancer, D-Limonene, Ganoderma applanatum, Oman, γ-Terpinene",
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T1 - Chemical composition and anti-proliferative effect of Oman's Ganoderma applanatum on breast cancer and cervical cancer cells

AU - Hakkim, Faruck L.

AU - Al-Buloshi, Mohammed

AU - Achankunju, Jackson

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Objectives To evaluate the anti-proliferative potential of Oman's Ganoderma applanatum on triple negative breast cancer cells (MDA-MB-231), cervical cancer cells (HEp-2) and Vero cells (normal) and its chemical profiling of G. applanatum. Methods The cytotoxicity of the methanolic extract of G. applanatum was tested on MDA-MB-231 cells, HEp-2 cells and Vero cells by microculture tetrazolium (MTT) assay. The anti-cancer properties of G. applanatum were characterized using gas chromatography coupled with gas chromatography mass spectrometry (GCMS) analysis. Results The methanolic extract of G. applanatum elicited dose-dependent cell death on both MDA-MB-231 and HEp-2 cells after 24 h of treatment, with an IC50 value of 84.6 μg/ml and 43.2 μg/ml, respectively. Normal vero cells were able to significantly withstand the treatment. Furthermore, seven molecules were identified from G. applanatum by GCMS analysis, and five of them were present in considerable amounts, namely, γ-terpinene (30.3%), d-limonene (23.6%), cis-2-methyl-4-pentylthiane-s,s-dioxide (15.3%), β-cymene (12.7%) and α-terpinolene (8.1%). Conclusion In this study, surprisingly, G. applanatum exhibited toxicity particularly towards tumour cells (MDA-MB-231 and HEp-2) compared to normal vero cells. This tumour-specific chemo-sensitivity of G. applanatum is unclear, and the mechanism of cancer cell death induced by G. applanatum should be studied in detail. In addition, the observed anti-cancer activity of G. applanatum might be due to the synergistic effects of the identified properties. This would pave the way for isolation and characterization of the potential anti-cancer molecule from Oman's G. applanatum.

AB - Objectives To evaluate the anti-proliferative potential of Oman's Ganoderma applanatum on triple negative breast cancer cells (MDA-MB-231), cervical cancer cells (HEp-2) and Vero cells (normal) and its chemical profiling of G. applanatum. Methods The cytotoxicity of the methanolic extract of G. applanatum was tested on MDA-MB-231 cells, HEp-2 cells and Vero cells by microculture tetrazolium (MTT) assay. The anti-cancer properties of G. applanatum were characterized using gas chromatography coupled with gas chromatography mass spectrometry (GCMS) analysis. Results The methanolic extract of G. applanatum elicited dose-dependent cell death on both MDA-MB-231 and HEp-2 cells after 24 h of treatment, with an IC50 value of 84.6 μg/ml and 43.2 μg/ml, respectively. Normal vero cells were able to significantly withstand the treatment. Furthermore, seven molecules were identified from G. applanatum by GCMS analysis, and five of them were present in considerable amounts, namely, γ-terpinene (30.3%), d-limonene (23.6%), cis-2-methyl-4-pentylthiane-s,s-dioxide (15.3%), β-cymene (12.7%) and α-terpinolene (8.1%). Conclusion In this study, surprisingly, G. applanatum exhibited toxicity particularly towards tumour cells (MDA-MB-231 and HEp-2) compared to normal vero cells. This tumour-specific chemo-sensitivity of G. applanatum is unclear, and the mechanism of cancer cell death induced by G. applanatum should be studied in detail. In addition, the observed anti-cancer activity of G. applanatum might be due to the synergistic effects of the identified properties. This would pave the way for isolation and characterization of the potential anti-cancer molecule from Oman's G. applanatum.

KW - Breast cancer

KW - Cervical cancer

KW - D-Limonene

KW - Ganoderma applanatum

KW - Oman

KW - γ-Terpinene

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