Changes in function of HIV-specific T-cell responses with increasing time from infection

Michel L. Ndongala, Philomena Kamya, Salix Boulet, Yoav Peretz, Danielle Rouleau, Cécile Tremblay, Roger Leblanc, Pierre Côté, Jean Guy Baril, Réjean Thomas, Sylvie Vézina, Mohamed R. Boulassel, Jean Pierre Routy, Rafick P. Sékaly, Nicole F. Bernard

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5 Citations (Scopus)

Abstract

Recently HIV-infected individuals have virus-specific responses characterized by IFN-γ/IL-2 secretion and proliferation rarely seen in chronic infection. To investigate the timing of loss of HIV-specific T-cell function, we screened cells from 59 treatment-naïve HIV-infected individuals with known dates of infection for proteome-wide responses secreting IFN-γ/IL-2 and IFN-γ alone by ELISPOT. HIV peptide-specific proliferation was assessed by carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution. The contribution of IFN-γ/IL-2 and IFN-γ-only secretion to the total HIV-specific response was compared in subjects infected <6, 6-12, and 12-36 mo earlier. The frequency of IFN-γ/IL-2-secreting cells fell, while that of IFN-γ-only secretion rose with time from infection. HIV peptide-specific proliferative responses were almost exclusively mediated by CD8+ T cells, and were significantly lower in cells obtained from the 12-36 mo versus < 6 mo post-infection groups. By the second year of infection there was a significant difference in these functions compared to those assessed within 6 mo.

Original languageEnglish
Pages (from-to)159-168
Number of pages10
JournalViral Immunology
Volume23
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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    Ndongala, M. L., Kamya, P., Boulet, S., Peretz, Y., Rouleau, D., Tremblay, C., Leblanc, R., Côté, P., Baril, J. G., Thomas, R., Vézina, S., Boulassel, M. R., Routy, J. P., Sékaly, R. P., & Bernard, N. F. (2010). Changes in function of HIV-specific T-cell responses with increasing time from infection. Viral Immunology, 23(2), 159-168. https://doi.org/10.1089/vim.2009.0084