Cardiovascular effects of nose-only water-pipe smoking exposure in mice

Abderrahim Nemmar, Priya Yuvaraju, Sumaya Beegam, Annie John, Haider Raza, Badreldin H. Ali

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Water-pipe smoking (WPS) is a major type of smoking in Middle Eastern countries and is increasing in popularity in Western countries and is perceived as relatively safe. However, data on the adverse cardiovascular effects of WPS are scarce. Here, we assessed the cardiovascular effects of nose-only exposure to mainstream WPS generated by commercially available honey-flavored "moasel" tobacco in BALB/c mice. The duration of the session was 30 min/day for 1 mo. Control mice were exposed to air. WPS caused a significant increase of systolic blood pressure (SBP) in vivo (+13 mmHg) and plasma concentrations of IL-6 (+30%) but not that of TNF-α. Heart concentrations of IL-6 (+184%) and TNF-α (+54%) were significantly increased by WPS. Concentrations of ROS (+95%) and lipid peroxidation (+27%) were significantly increased, whereas those of GSH were decreased (-21%). WPS significantly shortened the thrombotic occlusion time in pial arterioles (-46%) and venules (40%). Plasma von Willebrand factor concentrations were significantly increased (+14%) by WPS. Erythrocyte numbers (+15%) and hematocrit (+17%) were significantly increased. Blood samples taken from mice exposed to WPS and exposed to ADP showed significant platelet aggregation compared with air-exposed mice. WPS caused a significant shortening of activated partial thromboplastin time (-45%) and prothrombin time (-13%). We conclude that 1-mo nose-only exposure to WPS increased SBP and caused cardiac inflammation, oxidative stress, and prothrombotic events. Our findings provide plausible elucidation that WPS is injurious to the cardiovascular system.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume305
Issue number5
DOIs
Publication statusPublished - Sep 1 2013

Fingerprint

Nose
Smoking
Water
Blood Pressure
Interleukin-6
Air
Erythrocyte Count
Honey
Venules
Partial Thromboplastin Time
Prothrombin Time
von Willebrand Factor
Arterioles
Cardiovascular System
Platelet Aggregation
Hematocrit
Adenosine Diphosphate
Lipid Peroxidation
Tobacco
Oxidative Stress

Keywords

  • Heart
  • Inflammation
  • Nose-only exposure
  • Oxidative stress
  • Thrombosis
  • Water-pipe smoking

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Cardiovascular effects of nose-only water-pipe smoking exposure in mice. / Nemmar, Abderrahim; Yuvaraju, Priya; Beegam, Sumaya; John, Annie; Raza, Haider; Ali, Badreldin H.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 305, No. 5, 01.09.2013.

Research output: Contribution to journalArticle

Nemmar, Abderrahim ; Yuvaraju, Priya ; Beegam, Sumaya ; John, Annie ; Raza, Haider ; Ali, Badreldin H. / Cardiovascular effects of nose-only water-pipe smoking exposure in mice. In: American Journal of Physiology - Heart and Circulatory Physiology. 2013 ; Vol. 305, No. 5.
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