TY - JOUR
T1 - Cardioprotective potential of polyphenols rich Thraatchathi Chooranam against isoproterenol induced myocardial necrosis in experimental rats
AU - Ganapathy, Ramakrishnan
AU - Ramachandran, Anita
AU - Shivalingaiah, Sushmitha Basavapattana
AU - Bishir, Muhammed
AU - Bhojaraj, Saravanan
AU - Sridhar, Shivashree
AU - Mohan, Surapaneni Krishna
AU - Veeraraghavan, Vishnu Priya
AU - Chidambaram, Saravana Babu
AU - Essa, Musthafa Mohamed
AU - Qoronfleh, M. Walid
N1 - Funding Information:
The authors want to thank their respective institutions for their continued support. The technical and language editing done by The Editing Refinery, MD, USA is highly appreciated. Animal use complied with institutional, national, and international guidelines including the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Govt. of India guidelines. Guidelines of “Guide for the Care and Use of Laboratory Animals ” (Institute of Laboratory Animal Resources, National Academic Press 1996; NIH publication number #85–23, revised 1996) were strictly followed throughout the study. Institutional Animal Ethical Committee (IAEC) approved this study (IAEC-XXXVI/SRU/336/2013).
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12
Y1 - 2020/12
N2 - Background: The present study establishes the cardioprotective role of Thraatchathi Chooranam (TC), a polyherbal traditional Siddha medicine, in terms of membrane stabilizing and antioxidant properties in isoproterenol (ISO) induced myocardial necrosis model in rats. Methods: Animals were divided into six groups (n = 6), normal (received vehicle 0.5% CMC, p.o.), ISO control (received 0.5% CMC + ISO 120 mg/kg, b.w. s.c. twice at an interval of 48 h), standard control (received Vit-E 100 mg/kg, p.o.) + ISO, TC low and high dose (50 and 100 mg/kg p.o., respectively) + ISO, and drug control (received TC at 100 mg/kg, p.o.). At the end of experimental period, blood samples collected and plasma cardiac troponin-I (CTn-I) was measured by ELISA. Cardiac tissues were isolated, levels of membrane stabilizing enzymes, antioxidants and inflammatory markers were estimated. Gene expression of Bax, Bcl2, Caspase 3, HIF-α, TNF-α, iNOS, TRX1 and TrxR were performed by RT-PCR. Histopathological studies on cardiac tissues were conducted using hematoxylin and eosin (H&E) stain. Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparison as post-hoc test. Results: Administration of ISO resulted in a significant increase in plasma CTn-I, decrease in superoxide dismutase, glutathione and glutathione peroxidase; it also significantly altered membrane stabilizing enzymes like Na+/K+-ATPase, Mg2+-ATPase Ca2+-ATPase and Cathepsin D. Pretreatment with TC (50 mg/kg and 100 mg/kg) decreased CTn-I, and improved membrane stabilizing and endogenous antioxidant enzymes and decreased cathespin D level in a dose dependent manner. Histopathological examination revealed that TC improves cellular membrane integrity and decreases inflammatory cell infiltration and necrotic death. Conclusion: The present study provided a strong evidence on the protective effects of TC against ISO-induced myocardial necrosis in rats.
AB - Background: The present study establishes the cardioprotective role of Thraatchathi Chooranam (TC), a polyherbal traditional Siddha medicine, in terms of membrane stabilizing and antioxidant properties in isoproterenol (ISO) induced myocardial necrosis model in rats. Methods: Animals were divided into six groups (n = 6), normal (received vehicle 0.5% CMC, p.o.), ISO control (received 0.5% CMC + ISO 120 mg/kg, b.w. s.c. twice at an interval of 48 h), standard control (received Vit-E 100 mg/kg, p.o.) + ISO, TC low and high dose (50 and 100 mg/kg p.o., respectively) + ISO, and drug control (received TC at 100 mg/kg, p.o.). At the end of experimental period, blood samples collected and plasma cardiac troponin-I (CTn-I) was measured by ELISA. Cardiac tissues were isolated, levels of membrane stabilizing enzymes, antioxidants and inflammatory markers were estimated. Gene expression of Bax, Bcl2, Caspase 3, HIF-α, TNF-α, iNOS, TRX1 and TrxR were performed by RT-PCR. Histopathological studies on cardiac tissues were conducted using hematoxylin and eosin (H&E) stain. Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparison as post-hoc test. Results: Administration of ISO resulted in a significant increase in plasma CTn-I, decrease in superoxide dismutase, glutathione and glutathione peroxidase; it also significantly altered membrane stabilizing enzymes like Na+/K+-ATPase, Mg2+-ATPase Ca2+-ATPase and Cathepsin D. Pretreatment with TC (50 mg/kg and 100 mg/kg) decreased CTn-I, and improved membrane stabilizing and endogenous antioxidant enzymes and decreased cathespin D level in a dose dependent manner. Histopathological examination revealed that TC improves cellular membrane integrity and decreases inflammatory cell infiltration and necrotic death. Conclusion: The present study provided a strong evidence on the protective effects of TC against ISO-induced myocardial necrosis in rats.
KW - Antioxidants isoproterenol
KW - Apoptosis
KW - Cardioprotective
KW - Inflammation
KW - Membrane enzymes
KW - Oxidative stress
KW - Polyphenols
KW - Thraatchathi Chooranam
UR - http://www.scopus.com/inward/record.url?scp=85096446529&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85096446529&partnerID=8YFLogxK
U2 - 10.1186/s12906-020-03124-x
DO - 10.1186/s12906-020-03124-x
M3 - Article
C2 - 33225920
AN - SCOPUS:85096446529
SN - 1472-6882
VL - 20
JO - BMC Complementary Medicine and Therapies
JF - BMC Complementary Medicine and Therapies
IS - 1
M1 - 356
ER -