Cardioprotective potential of polyphenols rich Thraatchathi Chooranam against isoproterenol induced myocardial necrosis in experimental rats

Ramakrishnan Ganapathy, Anita Ramachandran, Sushmitha Basavapattana Shivalingaiah, Muhammed Bishir, Saravanan Bhojaraj, Shivashree Sridhar, Surapaneni Krishna Mohan, Vishnu Priya Veeraraghavan, Saravana Babu Chidambaram*, Musthafa Mohamed Essa, M. Walid Qoronfleh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The present study establishes the cardioprotective role of Thraatchathi Chooranam (TC), a polyherbal traditional Siddha medicine, in terms of membrane stabilizing and antioxidant properties in isoproterenol (ISO) induced myocardial necrosis model in rats. Methods: Animals were divided into six groups (n = 6), normal (received vehicle 0.5% CMC, p.o.), ISO control (received 0.5% CMC + ISO 120 mg/kg, b.w. s.c. twice at an interval of 48 h), standard control (received Vit-E 100 mg/kg, p.o.) + ISO, TC low and high dose (50 and 100 mg/kg p.o., respectively) + ISO, and drug control (received TC at 100 mg/kg, p.o.). At the end of experimental period, blood samples collected and plasma cardiac troponin-I (CTn-I) was measured by ELISA. Cardiac tissues were isolated, levels of membrane stabilizing enzymes, antioxidants and inflammatory markers were estimated. Gene expression of Bax, Bcl2, Caspase 3, HIF-α, TNF-α, iNOS, TRX1 and TrxR were performed by RT-PCR. Histopathological studies on cardiac tissues were conducted using hematoxylin and eosin (H&E) stain. Statistical analyses were performed by one-way ANOVA followed by Tukey’s multiple comparison as post-hoc test. Results: Administration of ISO resulted in a significant increase in plasma CTn-I, decrease in superoxide dismutase, glutathione and glutathione peroxidase; it also significantly altered membrane stabilizing enzymes like Na+/K+-ATPase, Mg2+-ATPase Ca2+-ATPase and Cathepsin D. Pretreatment with TC (50 mg/kg and 100 mg/kg) decreased CTn-I, and improved membrane stabilizing and endogenous antioxidant enzymes and decreased cathespin D level in a dose dependent manner. Histopathological examination revealed that TC improves cellular membrane integrity and decreases inflammatory cell infiltration and necrotic death. Conclusion: The present study provided a strong evidence on the protective effects of TC against ISO-induced myocardial necrosis in rats.

Original languageEnglish
Article number356
JournalBMC Complementary Medicine and Therapies
Volume20
Issue number1
DOIs
Publication statusPublished - Dec 1 2020

Keywords

  • Antioxidants isoproterenol
  • Apoptosis
  • Cardioprotective
  • Inflammation
  • Membrane enzymes
  • Oxidative stress
  • Polyphenols
  • Thraatchathi Chooranam

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Medicine(all)

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