Bioremediation of copper by active cells of Pseudomonas stutzeri LA3 isolated from an abandoned copper mine soil

Thenmozhi Murugaian Palanivel, Nallusamy Sivakumar, Aliya Al-Ansari, Reginald Victor*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Copper bioremoval efficiency and bioaccumulation capacity of Pseudomonas stutzeri LA3 isolated from copper contaminated soil were investigated. P. stutzeri LA3 removed about 50% of Cu (II) at 50 mg l−1 of concentration and accumulated a maximum of 1.62 mg of Cu g−1 biomass dry weight. Bioremediation by P. stutzeri LA3 partially depended on the production of extracellular polymeric substances, composed of proteins and carbohydrates. Cell surface alterations were observed on the Cu (II) treated biomass through a scanning electron microscope (SEM). Energy dispersive spectrometer (EDX) investigation of Cu (II) treated biomass showed clear signals of Cu, confirming the presence of copper ions on the cell surface. Fourier transform infrared spectroscopy (FTIR) showed the contribution of functional groups such as hydroxyl (–OH), carboxyl (–COOH), amide and amine (–NH2) in the remediation process. Based on the results, the isolated strain P. stutzeri LA3 could serve as a potential candidate for copper due to its significant copper removal effeciency.

Original languageEnglish
Article number109706
JournalJournal of Environmental Management
Volume253
DOIs
Publication statusPublished - Jan 1 2020

Keywords

  • Bioremediation
  • Copper
  • Extracellular polymeric substances
  • FTIR analysis
  • Pseudomonas stutzeri LA3
  • Scanning electron microscope

ASJC Scopus subject areas

  • Environmental Engineering
  • Waste Management and Disposal
  • Management, Monitoring, Policy and Law

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